Human CD42d ELISA Kit PicoKine®

Glycoprotein V/CD42d ELISA kit for Human

Human CD42d Kit PicoKine® (96 Tests). Quantitate Human GP5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK2211
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human CD42d ELISA Kit PicoKine®

View all Glycoprotein V/CD42d ELISA kits

SKU/Catalog Number

EK2211

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD42d Kit PicoKine® (96 Tests). Quantitate Human GP5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CD42d ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2211)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q17-G560

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK2211 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2211 is reactive to GP5 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2211 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Glycoprotein V/CD42d

Glycoprotein V (platelet) (GP5) also known as CD42d (Cluster of Differentiation 42d), is a human gene. Human platelet glycoprotein V (GP5) is a part of the Ib-V-IX system of surface glycoproteins that constitute the receptor for von Willebrand factor (VWF; MIM 613160) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX (GP9; MIM 173515) and GP5. Mutations in GP1BA, GP1BB, and GP9 have been shown to cause Bernard-Soulier syndrome, a bleeding disorde.

Kit Components

Catalog Number Description Quantity
EK2211-CAP Anti-Human GP5 Pre-coated 96-well strip microplate 1
EK2211-ST Human GP5 Standard 2 vials, 10 ng/tube
EK2211-DA Human GP5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)01563126251,2502,5005,00010,000
O.D.0.0530.1220.1960.4120.8721.2501.5632.055

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD42d ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)10846616351184741588
Standard deviation5.5120.9778.484.619.4376.22
CV(%)5.1%4.5%4.8%3.9%4.1%4.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Glycoprotein V/CD42d in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11081221171251187.26.1%
Sample 246646250148147826.265.5%
Sample 316351752169317271702100.45.9%
*number of samples for each test n=16.

Gene/Protein Information For GP5 (Source: Uniprot.Org, NCBI)

Gene Name

GP5

Full Name

Platelet glycoprotein V

Weight

60.959kDa

Alternative Names

platelet glycoprotein V; CD42d; glycoprotein V (platelet); Glycoprotein V; GP5; GPV GP5 CD42d, GPV glycoprotein V platelet platelet glycoprotein V|glycoprotein 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GP5, check out the GP5 Infographic

GP5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EK2211

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human CD42d ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Human CD42d ELISA Kit PicoKine®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Human CD42d ELISA Kit PicoKine®

$499
Backordered.

Lead time for this item is typically 2-3 weeks

Get A Quote
In stock
Order Product
EK2211
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.