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Product Info Summary
| SKU: | FEK1304 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human SIGLEC-1/CD169 PicoKine® Quick ELISA Kit
See all Siglec-1/CD169 products
SKU/Catalog Number
FEK1304
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD169/SIGLEC-1 PicoKine® Quick ELISA Kit (90 minues, 96 Tests). Quantitate Human SIGLEC1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human SIGLEC-1/CD169 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1304)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S20-Q1641
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1304 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1304 is reactive to SIGLEC1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK1304 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Siglec-1/CD169
SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1304-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1304-ST | Human Siglec-1/CD169 Standard | 2 | 10 ng/tube |
| FEK1304-A | Human SIGLEC1 Antibody A | 1 | 3ml |
| FEK1304-B | Human SIGLEC1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.085 | 0.159 | 0.191 | 0.268 | 0.494 | 0.754 | 1.313 | 2.051 |
Data Example Images
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Human Cathepsin V PicoKine ELISA Kitstandard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human SIGLEC-1/CD169 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 278 | 1821 | 4073 | 286 | 1650 | 3922 |
| Standard deviation | 20.01 | 138.39 | 240.3 | 22.59 | 148.5 | 262.77 |
| CV (%) | 7.2% | 7.6% | 5.9% | 7.9% | 9% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Siglec-1/CD169 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 278 | 276 | 287 | 263 | 276 | 8.57 | 3.1% |
| Sample 2 | 1821 | 2107 | 2081 | 1805 | 1953 | 140.91 | 7.2% |
| Sample 3 | 4073 | 4478 | 4741 | 4142 | 4358 | 268.77 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For SIGLEC1 (Source: Uniprot.Org, NCBI)
Gene Name
SIGLEC1
Full Name
Sialoadhesin
Weight
182.624kDa
Superfamily
immunoglobulin superfamily
Alternative Names
CD169; FLJ00051; sialic acid binding Ig-like lectin 1, sialoadhesin; sialoadhesin; Siglec1; Siglec-1 SIGLEC1 CD169, SIGLEC-1, SN sialic acid binding Ig like lectin 1 sialoadhesin|sialic acid binding Ig-like lectin 1, sialoadhesin|sialic acid-binding immunoglobulin-like lectin 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SIGLEC1, check out the SIGLEC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SIGLEC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human SIGLEC-1/CD169 PicoKine® Quick ELISA Kit (FEK1304)
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