Human C-Peptide OneStep ELISA Kit

Product Name

Human C-Peptide OneStep ELISA Kit

See all C-P products

SKU/Catalog Number

EK7039

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human C-peptide (C-P). 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human C-Peptide OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7039)

Clonality of Antibodies

See Datasheet for details

Sensitivity

0.25 ng/ml

Assay Range

0.5-8 ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7039 is reactive to C-P in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7039 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity	Volume	Buffers
Anti-Human C-peptide (C-P) Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Anti- C-peptide (C-P) monoclonal antibody, Polystyrene micro-well plate
Human C-peptide (C-P) Standards (S0~S5)	6	0.5ml	C-peptide (C-P) (0, 0.5, 1, 2, 4, 8) ng/ml, 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300
HRP Conjugated anti-Human C-peptide (C-P) antibody	1	6ml	HRP Conjugated anti-Human C-peptide (C-P) antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1%Proclin-300
Controls	2	0.5ml	C-peptide (C-P), 100% natural protein, 0.1% Proclin-300
20X Wash Buffer Concentrate	1	15ml	0.2M PBS, contains 0.5% tween 20
Color Developing Reagent A	1	7ml	11m mol/L Urea hydrogen peroxide
Color Developing Reagent B	1	7ml	2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution	1	7ml	2mol/L Sulphuric acid
Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

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