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Product Info Summary
| SKU: | FEK1357 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human Biglycan PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1357
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Biglycan PicoKine® Quick ELISA Kit (90 minues, 96 Tests). Quantitate Human BGN in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human Biglycan PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1357)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D38-K368
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1357 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1357 is reactive to BGN in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
FEK1357 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Biglycan
Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1357-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1357-ST | Human Biglycan Standard | 2 | 10 ng/tube |
| FEK1357-A | Human BGN Antibody A | 1 | 3ml |
| FEK1357-B | Human BGN Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.007 | 0.070 | 0.128 | 0.240 | 0.471 | 0.828 | 1.465 | 2.066 |
Data Example Images
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Human CCL16/HCC-4 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Biglycan PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 190 | 939 | 4176 | 179 | 981 | 4439 |
| Standard deviation | 9.12 | 54.46 | 167.04 | 10.2 | 56.89 | 217.51 |
| CV (%) | 4.8% | 5.8% | 4% | 5.7% | 5.8% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Biglycan in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 190 | 188 | 215 | 183 | 194 | 12.38 | 6.3% |
| Sample 2 | 939 | 960 | 955 | 1125 | 994 | 75.59 | 7.6% |
| Sample 3 | 4176 | 4429 | 4400 | 3916 | 4230 | 206.16 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For BGN (Source: Uniprot.Org, NCBI)
Gene Name
BGN
Full Name
Biglycan
Weight
41.654kDa
Superfamily
small leucine-rich proteoglycan (SLRP) family
Alternative Names
BGN; Biglycan; Bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; DSPG1; PGI; PG-S1; SLRR1A; SLRR1Abiglycan proteoglycan; small leucine-rich protein 1A BGN DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A biglycan biglycan|bone/cartilage proteoglycan-I|dermatan sulphate proteoglycan I|small leucine-rich protein 1A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BGN, check out the BGN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BGN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Biglycan PicoKine® Quick ELISA Kit (FEK1357)
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