Product Info Summary
SKU: | EK7107 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human, Avian |
Application: | ELISA |
Sample Types: | Cell Lysates, Tissue, Serum, Whole Blood |
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Product info
Product Name
HSC70 ELISA Kit
See all HSPA8/HSC71/Hsc70 products
SKU/Catalog Number
EK7107
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Colorimetric detection of HSC70. 96 wells/kit.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
HSC70 ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7107)
Clonality of Antibodies
See Datasheet for details
Sensitivity
1.54 ng/ml
Assay Range
2.34 - 150 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7107 is reactive to HSPA8 in Human, Avian samples
Validated Sample Types
Cell Lysates, Tissue, Serum, Whole Blood
Application Guarantee
EK7107 is guaranteed for ELISA in Human, Avian by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity |
Anti-Hsc70 Immunoassay Plate | 1 Plate |
5X Hsc70 Extraction Reagent | 1 vial/10 ml |
Recombinant Hsc70 Standard | 2 vials |
Standard and Sample Diluent | 1 vial/ 50 ml |
10X Wash Buffer Concentrate | 1 vial/100 ml |
Anti-Hsc70 Biotinylated Antibody Concentrate | 1 vial/150 µl |
Anti-Hsc70 Biotinylated Antibody Diluent | 1 vial/ 13 ml |
Streptavidin: HRP Concentrate | 1 vial/150 µl |
Streptavidin: HRP Diluent | 1 vial/ 13 ml |
TMB Substrate | 1 vial/ 13 ml |
Stop Solution | 1 vial/ 13 ml |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Typical Standard Curve for the HSC70 ELISA kit (Enzyme-Linked Immunosorbent Assay) -EK7107
Assay Type: Sandwich ELISA.
Detection Method: Colorimetric Assay.
Assay Range: 2.34 150 ng/mL.
Protein Target Info & Infographic
Gene/Protein Information For HSPA8 (Source: Uniprot.org, NCBI)
Gene Name
HSPA8
Full Name
Heat shock cognate 71 kDa protein
Weight
Superfamily
heat shock protein 70 family
Alternative Names
Heat shock 70 kDa protein 8; heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate 71 kDa protein; heat shock cognate protein 54; heat shock cognate protein, 71-kDa; HSC54; HSC70; HSC70heat shock 70kd protein 10; HSC71; HSP71; HSP73; HSP73constitutive heat shock protein 70; HSPA10; HSPA8; LAP1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; NIP71; N-myristoyltransferase inhibitor protein 71 HSPA8 HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71 heat shock protein family A (Hsp70) member 8 heat shock cognate 71 kDa protein|LPS-associated protein 1|N-myristoyltransferase inhibitor protein 71|constitutive heat shock protein 70|epididymis luminal protein 33|epididymis secretory sperm binding protein Li 72p|heat shock 70kDa protein 8|heat shock 70kd protein 10|heat shock cognate protein 54|lipopolysaccharide-associated protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPA8, check out the HSPA8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For HSC70 ELISA Kit (EK7107)
Hello CJ!
EK7107 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori
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