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Product Info Summary
| SKU: | LS001629 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein |
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Product info
Product Name
DBT (NM_001918) Human Over-expression Lysate
SKU/Catalog Number
LS001629
Size
100 µg
Description
Transient overexpression lysate of dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
DBT (NM_001918) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001629)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DBT Human Over-expression Lysates NM_001918.
Western blot validation of overexpression lysate (LS001629) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DBT (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for DBT (Source: Uniprot.org, NCBI)
Gene Name
DBT
Full Name
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Weight
53.487kDa
Superfamily
2-oxoacid dehydrogenase family
Alternative Names
BCATE2BCKAD E2 subunit; BCKADE2; BCKAD-E2; branched chain acyltransferase, E2 component; Branched-chain alpha-keto acid dehydrogenase complex component E2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto aciddehydrogenase complex; dihydrolipoamide branched chain transacylase (E2 component of branched chainketo acid dehydrogenase complex; maple syrup urine disease); dihydrolipoamide branched chain transacylase E2; Dihydrolipoamide branched chain transacylase; dihydrolipoyl transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase; E2 component of branche DBT BCATE2, BCKAD-E2, BCKADE2, BCKDH-E2, BCOADC-E2, E2, E2B dihydrolipoamide branched chain transacylase E2 lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial|52 kDa mitochondrial auto of primary biliary cirrhosis|BCKAD E2 subunit|E2 component of branched chain alpha-keto acid dehydrogenase complex|branched chain 2-oxo-acid dehydrogenase complex component E2|branched chain acyltransferase, E2 component|branched-chain alpha-keto acid dehydrogenase complex component E2|dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex|dihydrolipoyl transacylase|dihydrolipoyllysine-residue (2-methylpropanoyl)transferase|lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex|mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b)
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DBT, check out the DBT Infographic
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In this infographic, you will see the following information for DBT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DBT (NM_001918) Human Over-expression Lysate (LS001629)
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