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Product Info Summary
| SKU: | EK7119 |
|---|---|
| Size: | 96 wells/kit |
| Reactive Species: | All species |
| Application: | ELISA |
| Sample Types: | Dried Fecal Samples, Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media |
Product info
Product Name
Cortisol EIA Kit
SKU/Catalog Number
EK7119
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Quantitative colorimetric detection of cortisol. 96wells/kit.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Cortisol EIA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7119)
Clonality of Antibodies
See Datasheet for details
Sensitivity
17.3 pg/ml
Assay Range
100 - 3200 pg/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7119 is reactive to Cortisol in All species samples
Validated Sample Types
Dried Fecal Samples, Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media
Application Guarantee
EK7119 is guaranteed for ELISA in All species by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| Clear Microtitre 96 well Plate | 1 Plate |
| Cortisol Standard | 125 µl |
| Cortisol Antibody | 3 ml |
| Cortisol Conjugate | 3 ml |
| Assay Buffer | 50 ml |
| Dissociation Reagent | 1 ml |
| Wash Buffer Concentrate | 50 ml |
| TMB Substrate | 11 ml |
| Stop Solution | 5 ml |
| Plate Cover | 1 Each |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Typical Standard Curve for the Cortisol EIA Kit (Enzyme Immunoassay)K7119
Assay Type: Competitive EIA (Enzyme Immune Assay)
Detection Method: Colorimetric Assay
Assay Range: 100 3200 pg/ml
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Linearity was determined by taking two human urine samples diluted 1:140, one with a low diluted cortisol level of 163.9 pg/mL and one with a higher diluted level of 2,974.9 pg/mL and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.
Specific Publications For Cortisol EIA Kit (EK7119)
Hello CJ!
EK7119 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zheng PP, Zhang LN,Zhang J,Chang XM,Ding S,Xiao F,Guo LX.Evaluating the Effects of Different Sleep Supplement Modes in Attenuating Metabolic Consequences of Night Shift Work Using Rat Model. Nat Sci Sleep. 2020 Nov 20;12:1053-1065.doi:10.2147/NSS.S271318.
Species: Rat
EK7119 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA
Effect of one time coffee fragrance inhalation on working memory, mood, and salivary cortisol level in healthy young volunteers: a randomized placebo controlled trial.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Cortisol EIA Kit
Question
How many fecal samples can be processed by one EK7119 kit?
Verified customer
Asked: 2019-09-17
Answer
For the Cortisol EIA Kit EK7119, 40 samples in duplicate can be processed in one kit.
Boster Scientific Support
Answered: 2019-09-17
Question
Does EK7119 measure tetrahydrocortisol or free cortisol only?
Verified customer
Asked: 2019-04-05
Answer
At this time, we do not have a kit that can measure tetrahydrocortisols.
Boster Scientific Support
Answered: 2019-04-10
Question
What is the source of standard in the EK7119 kit? Does it contain any recombinant proteins and what is the expression system of each (e.g. E.coli, Baculovirus, CHO cells, etc. )?
Verified customer
Asked: 2018-09-06
Answer
The standard in this kit is a cortisol solution and Cortisol EIA Kit (EK7119) does not contain any recombinant proteins.
Boster Scientific Support
Answered: 2018-09-09
