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SARS-COV-2 (COVID-19) PCR KITS
COVID-19 Detection Kits, Primers/probes, RNA Extraction Kits
Popular Products
qPCR instruments
QPCR Thermo Cycler Real Time PCR System, Azure Cielo™ 3 And 6
Currently with only 6 weeks of lead time (may vary at the point of quote)
Compatible With The SarsCov2/COVID-19 Assays, Viral RNA DetectionThe Azure Biosystems™ Cielo™ 3 and 6 real-time PCR Systems are designed to offer smart protocols, high quality data, robust durability and an intuitive user experience.
Our novel fiber optic detection system allows for enhanced sensitivity and speed. 3 or 6 standard channels let you analyze a variety of commercially available dyes, already in your workflow. Onboard software operated by a 10.2″ touchscreen minimizes footprint and saves you bench space. Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Get notified after each completed run and even remotely access protocols and files.
- • Flexibility—Engineered for a wide variety of qPCR applications, with high sensitivity and wide dynamic range
- • Intelligent workflow—Our user interface allows simple touch screen assay setup. Saved protocols make assay set up easy and reproducible
- • Reliability—Cielo’s optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1000 qPCR experiments, giving you confidence in the reliability of the instrument
- • Connectivity—No external PC is required to run the instrument
- • Data can be transferred via Ethernet, Wi-Fi or USB
TC 9639 Gradient Thermal Cycler
- Intuitive touch screen control
- Program Wizard – quick, easy & foolproof
- Unique multi-format block: 0.2ml tubes or strips, 0.5ml tubes, 96 well plate
TC-32 Mini Thermal Cycler
- “Mini” sized, 9 x 7 inch footprint
- Capacity for 32 x 0.2ml or 4 PCR Strips
- Intuitive touch screen control
- Program Wizard with familiar icon driven programming
Detection reagents
Coronavirus Detection Kits And Primers
To help speed up the generation of a vaccine against the virus, Bosterbio is now providing ready-to-use primers and probes for COVID-19 (SARS-CoV-2, Coronavirus). These ready-to-use primers and probes will be made available to ship asap* with quick re-supply whenever necessary. We aim to prioritize all Coronavirus (COVID-19) related projects for as long as it is needed.
In addition, COVID-19 qPCR Detection Kits and Viral RNA Extraction Kits are now available.
SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex Real Time RT-PCR)
This real-time RT-PCR kit is intended for detection of ORF1ab and N genes from SARS-CoV-2 and for in vitro auxiliary diagnosis or emergency use of diagnosis of SARS-CoV-2 virus infection. The kit has a set of negative control, positive control and internal control.
- • High sensitivity: Limit of detection (LoD) of 0.5 copies/μl.
- • High specificity: Proprietary Triple Antibody Blocking-based Hot Start Technology. No cross reactivity with HCoV-HKU1, HCoV-229E, HCoV-OC43 and HCoV-NL63.
- • Fast workflow: Less than 60 min.
- • Triplex RT-qPCR: Dual target assay for ORF1ab and N genes and an internal control gene.
- • Minimized contamination : dUTP/ UNG used.
FocusGen Influenza Virus And SARS-CoV-2 Multiplex OneStep RT PCR Test Kit, No Need For Viral RNA Extraction
Influenza virus (IFV) A (a) and B (b) are the most common respiratory viruses in winter and spring. Detection of influenza virus and SAS-CoV-2 nucleic acid in cases of viral pneumonia is conducive to the etiological diagnosis of viral pneumonia. This kit is used for the on-site rapid diagnosis and screen of the Severe Acute Respiratory Syndrome Coronavirus 2 ( SAS-CoV-2) pneumonia and influenza virus (IFV) A/B by multiplex direct RT-PCR method without RNA extraction.
- • Detects influenza and COVID-19 in one test
- • No need for Viral RNA extraction
- • Fast, get results in under 60 minutes
- • Safe, avoid aerosol-based cross-contamination and prevents technician from infection.
COVID-19 Primers & Probes
Real time RT-PCR Primer list obtained from the United States Centers for Disease Control and Prevention (CDC) - last updated March 6, 2020.
Primers, probes, and protocol recommended by the World Health Organization (WHO) also available.
| Name | Sequence | Length | Final Yield | Purification | Modification |
|---|---|---|---|---|---|
| 2019-nCoV_N1-F | 5'-GAC CCC AAA ATC AGC GAA AT-3' | 20 | 2 OD | HPLC | |
| 2019-nCoV_N1-R | 5'-TCT GGT TAC TGC CAG TTG AAT CTG-3' | 24 | 2 OD | HPLC | |
| 2019-nCoV_N1-P | 5'-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3 | 24 | 2 OD | HPLC | 5'6-FAM, 3'BHQ1 |
| 2019-nCoV_N2-F | 5'-TTA CAA ACA TTG GCC GCA AA-3' | 20 | 2 OD | HPLC | |
| 2019-nCoV_N2-R | 5'-GCG CGA CAT TCC GAA GAA-3' | 18 | 2 OD | HPLC | |
| 2019-nCoV_N2-P | 5'-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3' | 23 | 2 OD | HPLC | 5'6-FAM, 3'BHQ1 |
| 2019-nCoV_N3-F | 5'-GGG AGC CTT GAA TAC ACC AAA A-3' | 22 | 2 OD | HPLC | |
| 2019-nCoV_N3-R | 5'-TGT AGC ACG ATT GCA GCA TTG-3' | 21 | 2 OD | HPLC | |
| 2019-nCoV_N3-P | 5'-FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1-3' | 24 | 2 OD | HPLC | 5'6-FAM, 3'BHQ1 |
| RNAse P (RP-F) | 5'-AGA TTT GGA CCT GCG AGC G-3' | 19 | 2 OD | HPLC | |
| RNAse P (RP-R) | 5'-GAG CGG CTG TCT CCA CAA GT-3' | 20 | 2 OD | HPLC | |
| RNAse P (RP-P) | 5'-FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ-1-3' | 23 | 2 OD | HPLC | 5'6-FAM, 3'BHQ1 |
| RdRP_SARSr-F2 | 5'-GTGARATGGTCATGTGTGGCGG-3' | 22 | 2 OD | HPLC | |
| RdRP_SARSr-R1 | 5'-CARATGTTAAASACACTATTAGCATA-3' | 26 | 2 OD | HPLC | |
| RdRP_SARSr-P2 | 5'-FAM-CAGGTGGAACCTCATCAGGAGATGC-DQ*-3' | 25 | 2 OD | HPLC | 5' FAM, 3' DQ* |
| RdRP_SARSr-P1 | 5'-CCAGGTGGWACRTCATCMGGTGATGC-DQ*-3' | 26 | 2 OD | HPLC | 5' FAM, 3' DQ* |
| E_Sarbeco_F1 | 5'-ACAGGTACGTTAATAGTTAATAGCGT-3' | 26 | 2 OD | HPLC | |
| E_Sarbeco_R2 | 5'-ATATTGCAGCAGTACGCACACA-3' | 22 | 2 OD | HPLC | |
| E_Sarbeco_P1 | 5'-ACACTAGCCATCCTTACTGCGCTTCG-DQ*-3' | 26 | 2 OD | HPLC | 5' FAM, 3' DQ* |
*: Probes may have an additional turnaround of 3-5 days.
More primers available than listed. Please inquire with our support team at [email protected].
Request More InformationCOVID-19 Viral RNA Extraction Kits
These Viral RNA Extraction Kits are especially designed for COVID-19 (SARS-CoV-2, Coronavirus) RNA Extraction. The kit simplifies the isolation of viral RNA from cell-free body fluids using a fast spin‐column format. No phenol/chloroform extraction is required. Viral RNA binds specifically while contaminants are removed in the flow-through. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in RNase‐free water. Purified RNA is ready to use in RT‐PCR, Northern blotting, or other downstream applications.
Kits are available in spin-column or magnetic bead formats.
| Description | Size |
|---|---|
| Spin Column Viral DNA/RNA Extraction Kit | 200 rxns |
| Magnetic Beads Viral DNA/RNA Extraction Kit | 50 rxns |
| FocusGen Influenza Virus And SASCoV2 Multiplex OneStep RT PCR Test Kit, No Need For Viral RNA Extraction | 25 Tests/50 Tests |
| RNA Extraction Kit | 50 rxns |
We offer these reagents in bulk order only, please contact us for pricing.
Get a QuoteGuanidine Thiocyanate
Guanidine Thiocyanate (aka guanidinium thiocyanate or guanidinium isothiocyanate), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria. When used with acid-equilibrated phenol or phenol:chloroform, a solution of guanidine thiocyanate and beta-mercaptoethanol is very effective in disrupting both cytoplasmic and nuclear membranes while maintaining the integrity of the RNA. In addition, guanidine thiocyanate can be used as a storage buffer for blood samples.
| Description | CAS | Size |
|---|---|---|
| Guanidine thiocyanate (GITC), DNase, RNase, Protease Free | 593-84-0 | 1 kg |
| Guanidine thiocyanate (GITC) | 593-84-0 | Custom |