ZFP36 Antibodies

mRNA decay activator protein ZFP36 (ZFP36)

Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by boosting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis (PubMed:9703499, PubMed:10330172, PubMed:10751406, PubMed:11279239, PubMed:12115244, PubMed:12748283, PubMed:15187101, PubMed:15634918, PubMed:17030620, PubMed:16702957, PubMed:20702587, PubMed:20221403, PubMed:21775632, PubMed:27193233, PubMed:23644599, PubMed:25815583). Acts as an 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery (PubMed:15687258, PubMed:23644599).

Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1, and thus boosts ARE-mediated mRNA deadenylation (PubMed:23644599). Functions also by recruiting components of the cytoplasmic RNA decay machinery to the jump ARE-containing mRNAs (PubMed:11719186, PubMed:12748283, PubMed:15687258, PubMed:16364915).

Gene Information

Human
Gene Name:	ZFP36
Uniprot:	P26651
Entrez:	7538

Family

Belongs to:
No superfamily

Alternative Names

EZNFTranscription factor 17; HKL1Zinc finger protein eZNF; KID-1; KID1TCF-17; TCF17; zinc finger protein 354A

Molecular Weight

Mass (kDA):
34.003 kDA

Genomic Context

Human
Location:	19q13.2
Sequence:	19; NC_000019.10 (39406847..39409407)

Tissue Specificity

Expressed in both basal and suprabasal epidermal layers (PubMed:27182009). Expressed in epidermal keratinocytes (PubMed:27182009). Expressed strongly in mature dendritic cells (PubMed:18367721). Expressed in immature dendritic cells (at protein level) (PubMed:18367721).

Subcellular Localizations

Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm, P-body. Shuttles between nucleus and cytoplasm in a CRM1-dependent manner (By similarity). Localized predominantly in the cytoplasm in a p38 MAPK- and YWHAB-dependent manner (By similarity). Colocalizes with SH3KBP1 and MAP3K4 in the cytoplasm (PubMed:20221403). Component of cytoplasmic stress granules (SGs) (By similarity). Localizes to cytoplasmic stress granules upon energy starvation (PubMed:15014438). Localizes in processing bodies (PBs) (PubMed:17369404). Excluded from stress granules in a phosphorylation MAPKAPK2-dependent manner (By s

Diseases

• Inflammation
• Neoplasms
• Malignant Neoplasms
• Arthritis
• Rheumatoid Arthritis
• Leukemia
• Autoimmunity
• Tumor Angiogenesis
• Hyperplasia
• Cachexia
• Pheochromocytoma
• Liver Carcinoma
• Obesity

• Diabetes Mellitus
• Autoimmune Diseases
• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Breast
• Nervousness
• Autoimmune Reaction
• Hypoxia

Interacting Proteins

• DCP1A
• EDC3

Pathways

• Localization
• Translation
• Secretion
• Cell Cycle
• Pathogenesis
• Cell Proliferation
• Mrna Destabilization
• Cytokine Production
• Angiogenesis
• Immune Response
• Mrna Stabilization
• Cell Growth
• Cellular Localization

• Regulation Of Gene Expression
• Nuclear Export
• Macrophage Activation
• S Phase
• Inflammatory Response
• Wound Healing
• Nuclear Import

PTMs

• Phosphorylation
• Dephosphorylation
• Biotinylation
• Cleavage

• Glycosylation
• Deacetylation
• Acetylation
• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ZFP36

Best Uses Of The ZFP36 Marker From Boster Bio

Boster Bio now has a new tool available for gene expression analysis. This tool allows users to analyze the genetic material of any organism, from plants to animals. It can be used for both human and mouse genes. The program's gene informationgraphics cover basic information about each gene, including its function and role in human and animal behavior. You can also use the search bar to locate specific genes.

AU-rich

Boster Bio not only manufactures its own antibodies and ELISA Kits, but also guarantees that their products will react to known targets. AU-rich Boster Bio provides a transparent product selection process through the use of Boster Badges, Bioz Citation Counters, and objective Bioz Stars ratings. All these features allow the company total transparency in their products and increase the reliability and trust of the products that they sell.

The company offers a large selection of picogram sensitive ELISA kits. They offer over 12,000 antibodies that were tested against panels of 250 tissue samples. To ensure high affinity, they also validated the antibodies against known amounts recombinant proteins as well as untransfected cell types. The Boster Bio portfolio includes a diverse range of AU-rich boster bio antibodies. This company has a wealth of experience in the field biotech products and can offer high-quality products that are suitable for many applications.

CU-rich

One of most intriguing questions in gene regulation research is whether the ZFP36 markers functions in context and cell dependent ways. The ZFP36 protein belongs to the CU family. There are many members. This gene is involved in many different processes. Translation and RNA stability are two examples. In addition to ZFP36/TTP, other members of this family include Tis11b, ERF-1, and BRF2. Although ZFP36/TTP has been extensively studied as an anti-inflammatory RNA stabilizing protein (RBP), recent studies have indicated that it may also play a tumor suppressor role.

In AD and psoriatic hair, the expression level of ZFP36 is decreased. The GSE121212 data set was used to normalize transcript level. qPCR was used to quantify ZFP36 expression within dermal fibroblasts. To determine the significance ZFP36 expression levels, qPCR analysis of the samples was done. ZFP36 marker is used for CU-rich purposes

U-rich

The anti-Psrc1 antibody from Boster Bio reacts with Aedes aegypti venom. This antibody is manufactured in-house. It will react with targets as predicted by its epitope-matching technology. The company offers a complete spike and recuperation protocol for its antibodies. They are formulated as 20mMPBS, 50% Glycerol, and 0.022% NaN3.

UUUUU pentamer sequences

Ten candidate amino acids were identified as a starting point in the construction of UUUUUUUUUU pentamers. Pent 2, the first of these, was named after a peptide number found in the sequence p210. This peptide corresponds at the epitope’s starting position. We then optimized staining, gating strategies using the apoE/ mice.

UTRs for 3'

Steven Boster designed the Boster Bio ZFP36 best uses guide. He is the inventor of hundreds of primary antibodies and developed various products for immunohistochemistry. By the late 90's, Boster had become the largest antibody catalog company in China. PicoKine(tm), his company's patented technology allows researchers to use his proprietary ELISA platform for high-sensitivity ELISA kit creations.

AREs

AU-rich elements (AUs), first discovered in the 3' UTR for labile mRNAs. AUs are composed primarily of pentamers and nonamers, with uridylates as a border. In general, AUs are unstable elements. AREs usually interact with RBPs. These include TIA1 (TIAR) and TIA1. Although there is no consensus on the function of these proteins, they all act as binding partners to AREs.

The amplitude of the enrichment was determined by measuring the signal-to-noise ratio of each sequence. The frequency of RREs was 40 to 250x higher than random chance at ZFP36 sites. These values were also double that of polyU-stretches similar in length. These findings, while there are still questions regarding the mechanism of ARERNA function, point to the existence complex HAEC metabolic pathways and gene regulatory pathways.

miRNAs

Recent years have seen researchers discover new uses for ZFP36. This gene is important for controlling mRNA abundance. It is capable of many biological functions and has been proven to be useful in many areas of biotechnology. Scientists are exploring new uses for this gene, such as the development of diagnostic tools and drug discovery. This article highlights a few potential applications.

The ZFP36 protein is a protein that is expressed primarily on macrophages. It is also involved in the immune response. In mice, ZFP36 KO cells respond to antigens with altered kinetics. Using HITS-CLIP and RNAseq to identify ZFP36 binding sites, researchers determined that ZFP36 was expressed at similar levels during the first four hours after stimulation. However, transcriptome profiling of ZFP36 KO-T cells revealed a highly divergent expression.

Target genes

The Boster Bio ZFP36 marker targets genes in a variety of tissue types, including prostate, breast, pancreatic, and lymphoma. Its highly targeted binding pattern targets all ELAVL1 ELAVL1 areas and can target gene in human or mouse cell. The gene infographics at bosterbio.com can help you pinpoint which genes this marker should target. The gene informationgraphics provide basic information on each of the genes. The formula of power-curve fit is used for determining the marker’s specificity.

Boster Bio ZFP36 was not biased by library generation biases. Although both markers bind a set of RNA sequences that overlap, they have different relative preferences. This could influence the combinatorial regulatory ability. This finding lays out a strategy to unravel in vivo combinatorial regulation of RNA-binding proteins. This approach may not be suitable for all samples.

Biological functions

Cell biology and molecular biology studies show that ZFP36 markers have diverse biological functions. One of these functions includes the regulation and activation of T-cells. It is also well-known to regulate target proteins levels. However, its functions in the immune system are still unclear. These roles will be discussed in greater detail in the following article. To better understand these roles, we will discuss the biochemical processes that control ZFP36 expression.

ZFP36's role is governed by several mechanisms. It has been shown, that the protein inhibits expression of certain mRNAs. This can lead to inflammation. In one study ZFP36 expression was found to be higher in vascular epithelium cells that are exposed to atherosclerotic signs. ZFP36 expression was not significantly different in mice from the outer and inner arches. Furthermore, the gene is not regulated by hemodynamics and does not accumulate in atherosclerosis before it develops.

Specificity

Numerous RNA sequences, including CDS-specific, have been shown contain a region that is rich in the ZFP36 marker. This region, called CDS-specific, is thought to regulate mRNA abundance. In fact, a subset of CDS binding sites was detected by the ZFP36 marker, indicating that this protein is localized to a coding exon. This study also showed that ZFP36 was highly specific for the CDS region, which indicates that it is capable of detecting its target mRNA.

Furthermore, the findings suggest that the binding site of ZFP36 is critical to the function of T cells in the immune system. Studies of mice lacking ZFP36 have shown that they develop autoimmune diseases, which are caused by the immune system attacking healthy cells. Understanding the exact functions of ZFP36 could also lead to new treatments for autoimmune disorders. The RNA-binding Protein is an important regulator of this delicate balance. It could also be used to treat diseases.