Protein Wnt-10a Antibodies

Protein Wnt-10a (Wnt10a)

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions at the canonical Wnt/beta-catenin signaling pathway.

Plays a role in ordinary ectoderm development. Required for normal tooth development.

Gene Information

Mouse
Gene Name:	Wnt10a
Uniprot:	P70701
Entrez:	22409

Family

Belongs to:
Wnt family

Alternative Names

FLJ14301; protein Wnt-10a; SSPS; wingless-type MMTV integration site family, member 10A; Wnt10a; Wnt-10a

Molecular Weight

Mass (kDA):
46.454 kDA

Genomic Context

Mouse
Location:	1|1 C4
Sequence:	1;

Tissue Specificity

Detected in foot plant epidermis, footpad epidermis, haired skin epidermis. Detected in adult epithelia, including filiform and fungiform papillae and sweat ducts. Detected in sweat gland myoepithelial cells, but not in sweat gland mesenchyme.

Diseases

• Malignant Neoplasms
• Neoplasms
• Ectodermal Dysplasia
• Carcinogenesis
• Malignant Neoplasm Of Stomach
• Dysplasia
• Hypodontia
• Malignant Neoplasm Of Breast
• Cell Transformation, Neoplastic
• Stomach Neoplasms
• Carcinoma
• Malignant Paraganglionic Neoplasm

• Malignant Neoplasm Of Pancreas
• Colorectal Cancer
• Palmoplantar Keratosis
• Embryonal Carcinoma
• Lymphoma
• Neoplasm Metastasis

Pathways

• Cell Proliferation
• Pathogenesis
• Localization
• Cell Migration
• Cell Differentiation
• Glycosylation
• Regeneration
• Dentinogenesis
• Cell Growth
• Osteoblast Differentiation
• Dentin Mineralization
• Limb Development
• Cell Cycle

• Dna Methylation
• Methylation
• Cell Adhesion
• Hair Follicle Morphogenesis
• Hair Follicle Development
• Dna Hypermethylation
• Gene Silencing

PTMs

• Glycosylation

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Wnt10a

Best Uses of the WNT10A Marker From Boster Bio

Boster Bio Anti WNT10A Picoband(tm), Antibody is available in multiple platforms. Validated with known positive or negative samples, the Anti-WNT10A Picoband Antibody has multiple validations. Boster Bio is committed towards ensuring high affinity and specificity. Boster also rewards scientists who review their products early with product credits. These benefits are available worldwide to all scientists. Here are some examples of the most popular uses for the WNT10A marker.

Boster Bio Anti-Wnt10a Picoband(tm) Antibody

Boster Bio Anti WNT10A Antibody PICOBAND(tm), which is mouse-specific and high-affinity, recognizes WNT10A. This antibody is available in Picoband(tm). It reacts with Human, Mouse and Rat cells. This antibody was raised against a synthetic protein of 14 amino acids and has been validated against WNT10A. The antibody recognizes WNT10A amino acids 300-355. This antibody is compatible in many other species, including Zebrafish and Tadpoles.

Immunofluorescence

Boster Bio has developed the WNT10A immunefluorescence marker. The antibody reacts with Human, Mouse, and Rat samples and is supplied in PBS with 0.02% sodium azide. This antibody reacts to a synthetic peptide that contains 14 amino acids. It can recognize a region between amino acid 300 and 350. Different immunogen lengths have different blocking peptides.

After intra-articular ligand transplantation of WNT ligands, WNT10A was tested in human synovium. In vitro analysis showed WNT5B (rat model) suppressed osteogenesis, increased inflammatory cytokines expression, and aggravated joints degeneration. However, this marker does have a mild protective impact on cartilage integrity. It is thought that the stem cells found in OA synovium are involved in the development of OA. This suggests that dysregulation in the WNT signaling pathway can affect the functioning of SMSCs, and ultimately cartilage destruction.

WNT10A is a member of a large family called WNTs. They play important roles in human development before birth. They provide instructions for building proteins and participate in chemical signaling pathways. They regulate the activity of genes and the interactions of cells during embryonic growth. WNT10A gene proteins are found in many tissues including skin, hair, nails and sweat glands.

WNT10A was expressed by human lymphocytes in vitro and macrophages in vivo. It is less prevalent in monocytes and more specific than MIF2 or CSF2. It is not known if WNT10A is expressed by human lymphocytes or mouse BMDMs. The WNT10A indicator for immunofluorescence provides powerful tools for analyzing immune system.

WNT10A, a key ligand, is involved in the regulation adult epithelial cell differentiation and proliferation. Activating the downstream b-catenin pathway may help repair the regenerative defects of WNT10a patients. This marker also supports a range of studies, including molecular imaging and gene therapy. It is available online at Boster Bio.

After blocking cells at RT for 2 h, the secondary antibody could be added. After 1 h, cells were observed under a fluorescence microscope. To determine cell viability, the results were compared to the original results. The WNT10A gene was found to be well-correlated with WNT10A expression in cancer cells. If the results are positive, it means that cancer cells have been identified in the corresponding tumors.

The WNT10A protein was identified in various tissue samples. The protein is found in the footpad epidermis (the skin), and the tongue filiform.papillae (the tongue). Human WNT10A patients show symptoms of scaling and cracking of the skin. Their mutations are due to mutations in KRT9 which is a gene expressed within the footpad epidermis.

Boster Bio's WNT10A antibody for immunofluorescence has been tested against many human antigens. This antibody will allow for immunofluorescence staining of human cells. Multiple platforms have also demonstrated the antibody’s specificity and affinity. Boster also rewards scientists who review the product by giving them product credits. WNT10A antibody immunofluorescence antibodies can be used by scientists to easily identify their research results.

Boster Bio has a WNT10A antibody that can be used to detect the presence of p–JNK. WNT proteins regulate many aspects in the body, including cell differentiation and cell growth. In addition, WNT proteins control the production of a variety of other proteins, including b-catenin and c-Jun N-terminal kinase.