Protein-glutamine gamma-glutamyltransferase E (TGM3)

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in a variety of proteins, in addition to the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes.

Catalyzes little proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are additional cross-linked by TGM1 on the growing CE scaffold (By similarity). In hair follicles, involved in cross- linking structural proteins to hardening the inner root sheath.