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- Table of Contents
Facts about Alpha-tectorin.
Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals. .
Human | |
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Gene Name: | TECTA |
Uniprot: | O75443 |
Entrez: | 7007 |
Belongs to: |
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No superfamily |
alpha-tectorin; DFNA12; DFNA8; DFNB21; tectorin alpha
Mass (kDA):
239.527 kDA
Human | |
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Location: | 11q23.3 |
Sequence: | 11; NC_000011.10 (121101243..121191490) |
Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Secreted, extracellular space, extracellular matrix. Found in the non-collagenous matrix of the tectorial membrane.
Boster Bio optimizes ELISA tests for maximum performance. Continue reading to learn how to make use of this immunoassay kit that is protein-based. You will also learn about High affinity primary antibodies, Tectorial membrane, and TECTA Marker. Common questions will also be answered. Boster Bio optimization guides and tips can be found to help you get more out of your experiments.
There are many options when it comes to ELISA. Which sample preparation process is best? Which blocking buffer works best? There are many options when it comes choosing which type of blocking buffer to use. For more Boster Bio optimization tips, read on. Don't forget the Boster bio optimization guide. It is packed with useful information and tips to optimize the performance of your experiments.
High-affinity primary antibody are those that bind to one epitope on a target protein. They are highly specific and possess high affinity. This makes them useful in purification, detection and further development. Primary antibodies are often created with animals as hosts. They may be of low affinity or high specificity. Both methods have their benefits and drawbacks. Here are some important facts to remember.
TECTA stands out as Tactical Enzyme Conjugated TrapArray. This technique involves printing duplicate spots of peptide on a microarray, and measuring their on/off rates. The on-rate indicates the speed at a target antibody can bind. KD is a measure the antibody's affinity for its target.
TECTA was created in order to identify a protein in the cells' body. The antibody binds to TECTA markers and is highly specific against them. The TECTA marker was used first to identify a protein called p62. This is a marker for autophagy. The primary antibody binds to p62, and the immunohistochemical staining method used for this analysis used normal goat serum with biotin-anti-mouse secondary antibody. The absence of the primary antibody p62 results in less staining or background staining in most cells.
This method is more sensitive and specific than other techniques as it relies upon the recognition of small sequences of peptides within proteins. The antibodies are able recognize the same epitopes of the antigen but at a higher level. This method may yield false-positive results, but it has a better chance of detecting immunomimetic sequencings. To avoid false-positive results, it is important that you first block the staining before you examine immunomimetic sequences.
The authors of antibody studies should note that reference to another work may not be sufficient to show that the antigens were immunogenically active. They should also mention that their antibodies were prepared with high dilutions to eliminate non-specific interactions. Signal attenuation can be caused by short incubation times. It is therefore crucial to provide thorough documentation. You run the risk of having your research rejected if there is not enough information.
The tectorial membrane is made up of two types of filaments: a-tectorin and b–tectorin. Both forms have the exact same structure and function. The a-tectorin forms homomeric fibers via the ZP Domain, while the B-tectorin is embedded within a striated-sheet matrix. Boster Bio uses rabbit and mouse as the source of its antibodies for this membrane.
PMID: 9590290 by Verhoeven K., et al. Mutations in the human alpha-tectorin gene cause autosomal dominant non-syndromic hearing impairment.
PMID: 21368133 by Zheng J., et al. Carcinoembryonic antigen-related cell adhesion molecule 16 interacts with alpha-tectorin and is mutated in autosomal dominant hearing loss (DFNA4).