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- Table of Contents
Facts about Transcriptional enhancer factor TEF-4.
Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'- GTGGAATGT-3').
Human | |
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Gene Name: | TEAD2 |
Uniprot: | Q15562 |
Entrez: | 8463 |
Belongs to: |
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No superfamily |
TEA domain family member 2TEF4ETF; TEAD-2; TEF-4; transcriptional enhancer factor TEF-4
Mass (kDA):
49.243 kDA
Human | |
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Location: | 19q13.33 |
Sequence: | 19; NC_000019.10 (49340595..49362452, complement) |
Nucleus.
This article will focus on the Boster bio Anti-TEAD2 Antibody Picoband as well as the Boster Bio Anti-Versican/VCAN antibody. We will also review the best uses of primary antibodies that have high affinity. This article can aid you in choosing the appropriate antibody for your project. If we were unable to convince you yet this article can aid you in making your decision.
Boster Bio Anti-Versican Antibody Picoband (VCAN) is a rabbit polyclonal anti-virus that recognizes antigens from human as well as mice and rats. It is approved for use in Immunohistochemistry, Western Blot and other immunohistochemistry applications. This antibody is a great option for researchers who want to identify proteins that are responsible for various types of cancer.
To use the Versican/VCAN Antibody PicoBand solution, titrate it by adding 22% BSA or TBS. In addition, biotinylated Hyaluronan can be added to the solution for biophysical and chemical analysis. The resultant solution can be used as a surface coating for cells.
Picokine (tm), an ELISA platform, increases sensitivity to the picogram level. These kits can be validated in a variety of samples and are available for purchase. Picoband is backed by an additional polymer-based secondary antibody, Supervision which can save approximately 30 minutes of IHC. It is powered by insights into the design of immunogens and technical assistance from BeNeLux distributor Sanbio.
Purified versican has been utilized in biological studies to determine the functions and interactions of the protein. Immunohistochemical methods can help to contextualize studies by locating versican proteins. In addition to immunohistochemistry, immunocytochemistry of Versican protein is routinely conducted on formalin-fixed paraffin-embedded tissue to examine its distribution.
The Versican protein cores are identified by western blot or ligand the blot. Chondroitinase ABC digestion of the protein creates V0 and V1, which are approximately 500 kDa. These two proteins are then transferred to nitrocellulose. The nitrocellulose is then transferred to an apparatus for transblotting that is semi-dry. The membrane is then blocked using the 2% BSA in TBS.
There are several suppliers that offer Boster Bio Anti-TEAD2 markers. These antibodies target TEAdomain transcription factor 2, also referred to by ETF or TEF-4. These antibodies can also react with orthologs in mice, canines and monkeys. The antibody is able to detect TEA domains both in mouse and rat. Boster Bio Anti TEAD2 Marker is an excellent choice for research.
These primary antibodies with high affinity recognize the same protein in different targeted tissues and cells like their names suggest. This marker is useful in detecting proteins with similar molecular "shape" or sequence as immunogens. This permits researchers to find specific proteins that have high sensitivity and specific detection methods while keeping the background low. Other markers, such as the TEAD2 marker, were created with organic polymers and polysaccharides, such as Boster Bio's Super Vision Detection Kits.
Monoclonal antibodies , on the other side recognize multiple epitopes within the same antigen. Monoclonal antibody are less expensive than polyclonal and are more resistant to variation in antigen and rigorous washes. Monoclonal antibodies are also more stable in various salt concentrations and pH levels. However, they are more susceptible to variation from batch-to-bath. Because they are made by a DNA sequence they are adapted to specific epitopes of peptides.
High-affinity TEAD2 markers are also useful in immunocytochemistry. This kind of antibody binds to an antigen with high affinity and is able to be used at extremely high dilutions, which eliminates the possibility of nonspecific interactions. The antibody is also highly purified, which permits researchers to measure and analyze antigens without requiring another or a third antibody.
TEAD2 has an advantage in that it is not drifting. This is the primary cause of antibody variations from lot one to lot two. This means that the antibody will possess peak specificity. This antibody will be able to recognize and bind any antigen of any species, without causing any negative effects. Additionally it is used for high-affinity primary antibodies which makes them perfect for researchers who have strict quality control requirements.
Utilizing TEAD2 as a biomarker for TEAD2 antigen detection is crucial for researchers. This marker is an excellent tool for high-affinity detectionthat can identify a broad range of targets. Boster Bio's high-affinity primary antibodies recognize antigens with the TEAD2 mark. The high-affinity antibodies are highly sensitive, which means they're ideal for use in immunohistochemistry.
In addition to using the TEAD2 marker to identify antibodies, Boster Bio has also utilized this marker to increase the quality of their products. TEAD2 antibodies are highly specific and can thus detect a wide array of targets. The TEAD2 marker is a well-established method to determine the best antibodies for any given application. Researchers can easily assess the quality and purity of their antibodies across various research applications using the TEAD2 marker.
PMID: 8702974 by Jacquemin P., et al. A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain.
PMID: 18579750 by Zhao B., et al. TEAD mediates YAP-dependent gene induction and growth control.