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- Table of Contents
Facts about TATA-binding protein-associated factor 2N.
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Human | |
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Gene Name: | TAF15 |
Uniprot: | Q92804 |
Entrez: | 8148 |
Belongs to: |
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RRM TET family |
68kDa; hTAFII68; Npl3; RBP56TAFII68; RNA-binding protein 56; TAF(II)68; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor; TAF2NRBP56/CSMF fusion; TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56); TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA-binding protein-associated factor 2N; TBP-associated factor 15,68 kDa TATA-binding protein-associated factor
Mass (kDA):
61.83 kDA
Human | |
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Location: | 17q12 |
Sequence: | 17; NC_000017.11 (35809484..35847242) |
Ubiquitous. Observed in all fetal and adult tissues.
Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
When performing research using antibodies to the TAF15 marker, you need high-affinity, specific antibodies. Boster provides high-affinity, primary antibodies that have been widely used over the past 25 year. These antibodies have been validated on Western Blotting, Immunohistochemistry, and ELISA. This boster antibody can be used for many applications, including immunohistochemistry and Western Blotting.
Boster Bio's antibodies are the best choice if you're in search of the perfect antibody. These highly validated antibodies have been created in-house from clones of repute. These antibodies can be conjugated to a variety conjugates. These products can also used for IHC or immunoprecipitation. You can find the high-affinity prima antibodies that you need to meet your needs, regardless of whether you are looking for an antibody to detect a specific protein or analyze its behavior.
Boster Bio offers ELISA kits and research antibodies for various applications. These kits are highly sensitive and have a high level of specificity and sensitivity. Their products are approved for IHC/ELISA, WB, FC, and FC. They also offer rabbit polyclonal antibodies. Boster Bio will provide a secondary antibody free of charge with any primary antibody. Boster Bio's high affinity primary antibodies have been used for countless scientific studies. They can be used to detect a variety biomarkers in many different areas.
Boster Bio's rabbit MAbs are of the highest affinity. Their PCD platform is ideal for these applications. Boster Bio's proprietary technology screens individual cells using a specialized chemistry that inhibits B cells from secreting antibodies. The cells are then retained on cell membranes by Boster Bio. Boster Bio plasma cells are able to discover specific antibody targets using flurochrome conjugated Antigens.
Flow cytometry is used for many research purposes. Flow cytometry provides powerful tools for studying cell, particle, and protein-based samples. Boster offers high-affinity polyclonal and monoclonal antibodies for this purpose. These antibodies have received many high citations over their 25-year history. Boster is a high quality primary antibody that can be used for flow cytometry.
Although many polyclonal antibody are useful for research, not all of them are suitable for clinical use. They may not possess the high affinity required for in vivo detection and measurement of the target protein. Low protein levels and competing binding receptors can lead to this. Additionally, polyclonal antibodies have a high risk of lot-to-lot variability and are difficult to gain approval for clinical assays.
These high-affinity primary antibody are a great choice when you need precise results. They are prepared using a process called titration ELISA that tests the antibodies for specificity and affinity to a target antibody. The standardized ELISA test is used to screen for positive supernatants. Next, the selected antibody are subjected to limiting dilution subcloning. This ensures that they grow at equal rates in all testwells.
There were many methods to determine the affinity maturation limits for these primary antibodies. One method is to use GraphPad Prism software to perform nonlinear regression analysis. A representative collection of 50 Abs was taken for the two most recent vaccinations. The data were distributed around clearly defined mean values. For example, the temperature rose by 2.2 degrees C but did not change the geometric mean of kon. However, the geometries mean of koff grew 2.4-fold, which corresponds a significant drop at t1/2.
Boster Bio has a wide range of secondary antibodies that have high affinity against the TAM5 marker. These secondary antibodies can be used to speed up the sample preparation process and enhance your research. These antibodies are available in two formats from Boster Bio: monoclonal (or polyclonal). Boster antibodies are highly cited in scientific journals, and have been used for many studies for over 25-years.
The method used to create primary and secondary antibodies is what makes them different. Primary antibodies are made from rabbit polyclonal antibodies. Secondary antibodies can be produced by immunizing the host with an antigen from another species. A rabbit polyclonal antibody can be transformed into an antigen fragment that contains the target antibody's specificity. These antibodies are available for use as detection, purification, and measurement agents.
Dual labeling of specimens is possible with the primary and secondary antibodies. This allows you more information and allows you to ask questions about the specimen. This makes it easier for you to understand the cellular structure in a sample. The secondary antigen system allows you to analyse the cellular makeup in greater detail. Secondary antibodies are available in different conjugates, in addition to primary antibodies.
Boster Bio anti Transcription factor 15 TCF15 antibody has a high specificity and versatility. Developed in 1993, this product has been validated in both WB and ELISA. It reacts with both Humans as well as mice. The antibody is supplied in a 100 ul Liquid form. It can be used for both IP and WB assays.
Monoclonal antibodies show a greater affinity towards native proteins, while antibodies against denatured proteins have lower affinity. The WB results may not be sufficient evidence to prove the monoclonal antibodies' particularity. Additional validation can be obtained by performing IHC and flow cytometry experiments. Negative controls for IHC experiments are essential to determine whether secondary antibodies bind to the target antibody non-specifically.
The key component of immunoassays is secondary antibodies. They are used to detect antigens in a variety of tissues. For example, ELISA uses antibodies to identify the amount of certain cell types in a sample. Flow cytometry is used to determine the number of particular cell types in a patient’s blood. Boster Bio also offers a high-affinity secondary anti-TAF15 antibody.
PMID: 8954779 by Morohoshi F., et al. Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins.
PMID: 8890175 by Bertolotti A., et al. hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II.