SPOUT1 Antibodies

AND SPOUT1 ELISA kits, SPOUT1 Proteins

Many biological assays use antibodies to detect SPOUT1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with SPOUT1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop SPOUT1 antibodies...

We validate the specificity of these antibodies to SPOUT1 by testing them on tissues known to express SPOUT1 positively and negatively. Browse below to find the SPOUT1 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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SPOUT1 Infographic by Boster Bio

All SPOUT1 Products

Product

Figures

Specificity

Applications

Price

C9orf114 (SPOUT1) (NM_016390) Human Recombinant Protein

$1249
Cat # PROTQ5T280
20 µg

C9orf114 (SPOUT1) (NM_016390) Human Over-expression Lysate

Human

$628
Cat # LS051490
100 µg

SPOUT1 Overview

Facts about Putative methyltransferase C9orf114.

SPOUT1 3D structure. Source: alphafold

Putative methyltransferase C9orf114 (SPOUT1)

Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed:20813266, PubMed:25657325). Also involved in chromosome alignment (PubMed:20813266).

May promote centrosome maturation likely by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis (PubMed:25657325). Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional degree (PubMed:28431233).

Gene Information

Human
Gene Name:	SPOUT1
Uniprot:	Q5T280
Entrez:	51490

Family

Belongs to:

class IV-like SAM-binding methyltransferase superfamily

Alternative Names

Putative methyltransferase C9orf114

Molecular Weight

Mass (kDA):
42.009 kDA

Genomic Context


Human

Location:	9q34.11
Sequence:	9; NC_000009.12 (128819651..128833309, complement)

Subcellular Localizations

Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Associated with the outer kinetochore.

Interacting Proteins

INCA1

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SPOUT1

References

PMID: 20813266 by Ohta S., et al. The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.

PMID: 25657325 by Ohta S., et al. CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.