Transcription factor Sp3 Antibodies

Transcription factor Sp3 (SP3)

Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements.

Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping.

Gene Information

Human
Gene Name:	SP3
Uniprot:	Q02447
Entrez:	6670

Family

Belongs to:
Sp1 C2H2-type zinc-finger protein family

Alternative Names

DKFZp686O1631; GC-binding transcription factor Sp3; Sp3 transcription factor; Sp3; specificity protein 3; SPR2; SPR-2; transcription factor Sp3

Molecular Weight

Mass (kDA):
81.925 kDA

Genomic Context

Human
Location:	2q31.1
Sequence:	2; NC_000002.12 (173900775..173965702, complement)

Tissue Specificity

Ubiquitously expressed.

Subcellular Localizations

Nucleus. Nucleus, PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Traditional Serrated Adenoma
• Liver Carcinoma
• Tumor Angiogenesis
• Leukemia
• Neoplasm Metastasis
• Cell Transformation, Neoplastic
• Adenocarcinoma
• Neuroblastoma

• Inflammation
• Malignant Tumor Of Colon
• Malignant Paraganglionic Neoplasm
• Endometrial Polyp
• Fibrosis
• Retinoblastoma
• Nervousness

Interacting Proteins

• ESR1

Pathways

• Methylation
• Translation
• Cell Cycle
• Pathogenesis
• Angiogenesis
• Cell Proliferation
• Cell Growth
• Secretion
• Rna Interference
• Cell Differentiation
• Localization
• Dna Methylation
• Transport

• Histone Acetylation
• Regulation Of Gene Expression
• Cell Death
• Demethylation
• Gene Silencing
• Cell Cycle Arrest
• Dna Replication

PTMs

• Phosphorylation
• Methylation
• Acetylation
• Cleavage
• Oxidation
• Dephosphorylation
• Demethylation
• Deacetylation
• Sumoylation
• Amination

• Reduction
• Amidation
• Glycosylation
• Carboxylation
• Hydroxylation
• Deamination
• Decarboxylation
• Ubiquitination
• Formylation
• Glutathionylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SP3

Best Uses Of The SP3 Marker

The Boster Bio is a powerful tool for researchers who are seeking to optimize their experiments. In addition to optimizing experiments, this guide also provides useful tips and techniques for identifying and overcoming common problems that plague experiments with Boster Bio. Troubleshooting guides and optimization tips can help you identify and eliminate the most common causes of trouble and achieve the highest-quality results possible. They can also help you avoid common mistakes, such as those that affect the accuracy of results.

SDS-SP3 protocol used for label-free quantification

Label-free quantitative proteomics requires effective sample preparation and removal of contaminants. Several sample preparation methods have been evaluated for their efficacy in label-free quantification. A modified carboxylated SP3 workflow was evaluated for its effectiveness in label-free quantification using a wide range of protein inputs. It also possesses a simple, yet robust, 2-hr peptide digestion protocol that requires no further adjustment.

Several benchmark studies have compared the methods for label-free quantitative ELISA on human and animal tissues. However, few studies have addressed protein samples derived from plants. A study conducted by Song et al. (2018) evaluated three sample preparation protocols for A. thaliana green leaves, including in-solution and non-aqueous methods. Similarly, Wang et al. (2018) compared two FASP protocols using SDS.

For the SP3 Carboxy method, the mean number of recovered peptides and protein groups was determined for three replicates. Error bars for three replicates of the same sample showed technical variability of the protein groups. The S-Trap method exhibited the lowest CV, while SP3 HILIC and FASP showed the highest. Hence, it is recommended that one choose the most accurate and reliable label-free protocol for histology.

This label-free proteomics protocol was optimized to meet the requirements of the lung cancer research community. It offers the greatest overall coverage of lung cancer-relevant proteomes and demonstrates the highest reproducibility over four replicates. In addition, this method also allows for rapid comparison of different protein quantification methods, including TMT and MS2 based labeling. Once a sample is analyzed, it must be diluted and resuspended in a buffer for the best results.

The protein yields in different types of NSCLC FFPE tissues were examined. A 10 um slice of resected tissue produced 150 ug of protein. In contrast, a needle biopsy produced only 70 ug of protein. In single-shot analyses of all three protein extraction protocols, over 3000 proteins were identified. In addition, the SDS-SP3 protocol was more efficient than DTR. Its mean CV was lowest in the SDS-SP3 protocol.

Applications of the SDS-SP3 Marker

SP3 is a commercially available protein and peptide immobilization agent. It immobilizes proteins in a non-oxidizing environment. The solvation layer promotes SP3 immobilization by trapping proteins in an aqueous solvation layer and increasing the concentration of organic additives in the sample preparation solution. After immobilization, proteins and peptides bound to the beads can be processed or rinsed. They can be used for a variety of applications.

The SP3 method detects the most protein, with an average of 3152 proteins per sample. The iST method detected 2343 proteins, and the FASP method detected 109 proteins. The SP3 method is suitable for the analysis of low amounts of starting materials. The iST and FASP methods do not yield high-quality results. The SP3 method is more reliable and accurate than the other two methods, especially when the starting material is ultra-low.

The SP3 method has been used successfully in the diagnosis of ovarian cancer and colorectal cancer. However, it is important to remember that this method is not foolproof, and the SP3 method is not intended for use on animals. It is highly recommended to consult with a physician before using the method. The SP3 method is highly accurate, and has been widely adopted in several clinical settings. It is therefore an invaluable tool for determining the extent of cancer in human samples.

Optimization tips

For most researchers, there are numerous questions about SP3 immunohistochemistry (IHC) experiments. The help of a Boster Bio optimization guide can help answer these questions and more, as well as improve the results of your experiments. No experiment is perfect, and researchers often run into errors along the way. But, by identifying the possible sources of error, you can minimize the impact of these missteps and improve your results.