SP100 Antibodies

Nuclear autoantigen Sp-100 (SP100)

Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a high number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 based on PubMed:11909962.

Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA based on PubMed:15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion.

Gene Information

Human
Gene Name:	SP100
Uniprot:	P23497
Entrez:	6672

Family

Belongs to:
No superfamily

Alternative Names

DKFZp686E07254; FLJ00340; FLJ34579; lysp100b; nuclear antigen Sp100; nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; SP100 nuclear antigen; SP100-HMG nuclear autoantigen; Speckled 100 kDa

Molecular Weight

Mass (kDA):
100.417 kDA

Genomic Context

Human
Location:	2q37.1
Sequence:	2; NC_000002.12 (230416156..230545606)

Tissue Specificity

Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines.

Subcellular Localizations

Nucleus. Nucleus, PML body. Cytoplasm. Differences in the subnuclear localization of the different isoforms seem to exist and may also be cell cycle- and interferon-dependent. Accumulates in the cytoplasm upon FAS activation.; [Isoform Sp100-C]: Nucleus. Forms a reticulate or track-like nuclear pattern with denser concentrations at the nuclear lamina and surrounding the nucleoli, a pattern reminiscent of heterochromatin-rich regions according to PubMed:11313457.

Diseases

• Leukemia
• Acute Promyelocytic Leukemia
• Biliary Cirrhosis
• Primary Biliary Cirrhosis
• Liver Cirrhosis
• Autoimmune Reaction
• Virus Diseases
• Cytomegalovirus Infections
• Infective Disorder
• Liver Diseases
• Neoplasms
• Autoimmune Diseases

• Hepatitis
• Malignant Neoplasms
• Leukoencephalopathy, Progressive Multifocal
• Hepatitis, Autoimmune
• Cholangitis
• Dental Plaque
• Autoimmunity

Interacting Proteins

• ACTN2
• CASP8AP2
• CBX5
• DNMT3A
• DYRK2

• ETS1
• GIPC2
• L3MBTL3
• RTP5
• SUMO1

• SUMO3
• TRAF3IP3
• ZC2HC1A

Pathways

• Localization
• Pathogenesis
• Viral Replication
• Dna Replication
• Cell Cycle
• Immune Response
• Conjugation
• Interphase
• Dna Repair
• Mitosis
• Rna Interference
• Cell Proliferation
• Senescence

• Oncogenesis
• Transport
• Cell Growth
• Telomere Maintenance
• Nuclear Import
• Methylation
• Virus-host Interaction

PTMs

• Sumoylation
• Phosphorylation
• Methylation
• Ubiquitination

• Acetylation
• Reduction
• Deamination
• Cleavage

• Citrullination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SP100

Boster Bio - Best Uses Of The SP100 Marker

This article will discuss the advantages of the SP100 Marker and the anti-SP100 Campaign. These methods offer many benefits, including a simplified Control System design and easier testing procedures. Ultimately, you'll get the most out of the SP100 Marker and its many benefits. Continue reading for more information. Find out why Boster Bio's SP100 markings are the best option for your laboratory.

Benefits of using the SP100 Marker

If you're looking for a better way of assessing your students' performance in SP100, the SP100 marker may be the best option. The SP100 course will help students communicate effectively and increase their confidence speaking to large audiences. Students will learn how they can organize and deliver speeches, and how to evaluate information. They will also learn about citations and how to use them in their speeches.

Another benefit of the SP100 marker's ability to identify the type and strength of the immune system of pigs is its ability. This marker is easy to use and does NOT depend on the environment. It can also be used to identify early-seed selection. The SP100 marker can be used to identify specific traits in pigs. This will help improve their productivity and immune system. It is also easy-to-interpret.

Control system design simplification

The present concept uses a series of gas-insulated solid-wall alcoaxial tubes. This proved the basic feasibility for an SP-100-powered Space Station. This concept also shows how reel-deployed dielectrically insulated coaxial cable can be used in launch environments with radiation and electrical stresses exceeding 50 kV/cm. Although the design of this new concept remains in its early stages, it shows that a SP-100-powered Space Station is possible.

Recent research in lower power, generic early applications has led to simplification of the control systems. The paper discusses non-mission-dependent system features, such as digital computer-based controllers, dual-purpose control rods and drives, and neutron flux monitors. Thawing systems are mission dependent features. They can either be electrical, or based in NaK tracelines.

Anti-SP100 Marker

The anti-SP100 marker boster bio is an antibody that reacts with Human. The antibody can be stored at -20C for up to a year, and at 4C for one month. It can be validated on WB, IHC, ICC, Immunofluorescence, and ELISA. Antibodies for Sp100 are typically purchased from Boster Bio.

This antibody binds to sp100 nuclear antigen molecularly and is a monoclonal antigen. The antigen recognizes the human Sp100 amino acid 209-227. It also recognizes cDNAs and peptides. This antibody cannot detect human biliary dysfunction. It can however recognize other targets and is therefore a great choice for diagnosing liver disease.

SP100, also known LYSP100B in the nucleus, is a subnuclear, or organelle. This protein has been covalently modified using SUMO-1. This modification is critical for nuclear bodies interaction. SP100 binds heterochromatin proteins, which may contribute to tumorigenesis. Interferon stimulates the synthesis of SP100, and it has been shown to act as a transcriptional coactivator and corepressor of ETS1.

Anti-SP100 antibody reacts to full-length Sp100 protein. It is useful in diagnosing non-hepatic Anti-Sp100 patients. Anti-Sp100 antibodies are often associated with AMA negative patients and PBC. Anti-SP100 antibodies are often found in combination with anti-PML antibodies. The anti-SP100 antibodies and anti-PML antibody are usually found in the same serum.

This test is very specific for PBC and sensitive. It is also specific for liver disease. It is also less specific for PBC and more sensitive than anti MND. This is why anti SP100 is an excellent marker for both types. As with other tests, anti–SP100 antibody positivity can be associated with higher gammaglobulin levels as well as older age. However, anti-MND is not linked to clinical profile.

The Sp110 amino acids sequence has been identified. It includes a Sp100-like domain and a SAND domain. There is also a bromodomain. Studies in the past have shown that Sp110 plays a role at homodimerization. It is possible for the SP110 protein to interact with the SP100- and Sp140-proteins. What is the interaction between them? These interactions can be recognized by the Sp110 antibody.