Sox17 Antibodies

Transcription factor SOX-17 (Sox17)

Binds to the sequences 5'- AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation through WNT3A.

Inhibits Wnt signaling. Promotes degradation of activated CTNNB1.

Gene Information

Mouse
Gene Name:	Sox17
Uniprot:	Q61473
Entrez:	20671

Family

Belongs to:
No superfamily

Alternative Names

FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3

Molecular Weight

Mass (kDA):
44.646 kDA

Genomic Context

Mouse
Location:	1 A1|1 1.65 cM
Sequence:	1;

Tissue Specificity

Testis. Detected in lung.

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Diabetes Mellitus
• Embryonal Carcinoma
• Testicular Neoplasms
• Seminoma
• Neoplasm Metastasis
• Malignant Neoplasm Of Breast
• Tumor Angiogenesis
• Germ Cell Tumor
• Malignant Neoplasm Of Stomach
• Cell Transformation, Neoplastic

• Diabetes Mellitus, Experimental
• Pathologic Neovascularization
• Idiopathic Hypereosinophilic Syndrome
• Carcinogenesis
• Neoplasms, Germ Cell And Embryonal
• Bisphosphonate-associated Osteonecrosis
• Hyperplasia

Pathways

• Gastrulation
• Endoderm Formation
• Cell Differentiation
• Methylation
• Endoderm Development
• Angiogenesis
• Cell Proliferation
• Pathogenesis
• Pancreas Development
• Stem Cell Differentiation
• Cell Cycle
• Localization
• Secretion

• Sex Determination
• Dna Methylation
• Liver Development
• Vasculogenesis
• Spermatogenesis
• Regeneration
• Nodal Signaling Pathway

PTMs

• Methylation
• Phosphorylation

• Demethylation
• Cleavage

• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Sox17

Best Uses Of The SOX17 Marker

The SOX17 subunit of adenosine diphosphate (Adenosine triphosphate) plays an important part in vascular disease. As such, a ELISA kit targeting SOX17 can be an effective tool for disease diagnosis. The Picokine(tm) platform allows for enhanced sensitivity down to the picogram level. The ELISA kits can be used for large samples. You can request the validation process and images to support your application. Picoband is powered in part by Sanbio's insight into the design of immunogens.

IHC-optimized Polyclonal Antibodies

Boster Bio IHC-optimized-polyclonal antibodies recognizing the SOX17 marker are a new generation of monoclonal antibodies with improved specificity and sensitivity. This IHC-optimized, polyclonal antibody can recognize a variety hematopoietic antigens thanks to this unique marker. This allows researchers to target an antigen more precisely and improve patient outcomes.

ES cell lines expressing SOX17

This study used a modified protocol to generate ES cell lines expressing Sox17. The cDNA for Sox17 came from the Harvard Institute of Proteomics. It was then ligated to the 3XFlag sequence by Sigma. Then, the cDNA was subcloned into a Sox17-flip-in vector, which contained a frt-flanked neomycin selectable marker and a promoterless hygromycin resistance gene. Using a Gene PulserII, 50 mg of Sox17-flip-in vector was electroporated into 1 x 107 KH2 ES cells.

Functional studies were done in the laboratory using Sox17/ES cells. However, the Sox17/XEN cell line was not possible to generate. This approach is particularly useful since Sox17-/ES cell lines are able to be used as an in vitro model for cavitation in the embryo early. EBs contain cells that express Nanog and also primitive endoderm gene expression.

LIF-supplemented ESCs were grown for 10 d in medium without Tet. Control EBs were prepared with the same procedure but in the presence of Tet. Arrows represent vacuoles and asterisks indicate basement membrane-like structures. These ESCs were then analyzed and compared to Gapdh for Sox17 expression. ExEn gene expression also requires Sox17.

Sox17 is required for differentiation of ES cells. We used two Sox17+/ES-cell lines to generate embryonic body embryos. We found that Sox17Tomato protein expression was always located at the perimeter of control EBs, and adjacent to the epithelialized bottom membrane. The mutant EBs displayed highly disorganized Sox17 expression which indicated that their cell sorting abilities were severely impaired.

In vitro studies have demonstrated that Sox17 plays a functional role in the differentiation of ES cell into the ExE lineage. While Sox factors are crucial for the early stages of ESC differentiation into ExE lineage, the late stage of this differentiation does not depend on Gata factor. Gata factor and Sox17 are also necessary for the differentiation ES cells to PE or VE.

ELISA kits that target SOX17

ELISA kits targeting SOX17 are designed to detect this protein by its tyrosine phosphorylation at residue 259. This biomarker is critical for the diagnosis and treatment of CRPC. SOX17 is up-regulated by many cancers, which may lead to tumorigenesis. SOX17 overexpression leads to increased tumor cell proliferation and angiogenesis. This makes SOX17 a potential target of CRPC therapy.

The MBS9318151 SOX17 ELISA kit is a ready-to-use microwell strip plate ELISA designed for the detection of SOX17 in biological samples. The ELISA kit uses the SOX17 antibody/SOX17 antigen interaction in order to detect SOX17 within biological samples. This kit can be used with both undiluted and diluted samples. The detection range is between 10 and 50 ng/mL.

One study showed that patients with CRPC were categorized into those with or without SOX17. Sox17 knockdown decreased Notch2 and Notch2 receptor expression. Conversely, SOX17 knockdown resulted in increased expression of Notch receptors Notch3 & Notch4, indicating that SOX17 negatively regulates Notch-signaling. These findings are further supported with a number ELISA kits against SOX17.

Additionally, SOX17 mutations were reported in sporadic VUR. Two families with 158 members were studied and found that they shared the same SOX17 p.Y259N mutation. The pedigrees of these families are shown in Figure 1A. FIGURE 1A illustrates the pedigrees of these families.

ELISA kits that target SOX17 can also detect protein expression in the mouse urogenital system. SOX17, one of 38 genes on chromosome 8, is expressed in mouse urogenital track development. SOX17 is important for endoderm formation and has been shown to play a role on signaling, regulatory pathways. One study found that a SOX17-mutant proband had an abnormal corpus callosum, which was associated with a 13-Mb duplication in over 30 genes.

Furthermore, SOX17 expression was significantly greater in CRPC tissue than in prostate tumors. However, tumor metastasis was associated with SOX17 expression in CRPC. Moreover, SOX17 expression was associated with increased expression of Notch2 and Notch4, but Notch3 and Notch4 were not significantly different. This suggests that the SOX17 gene could play a role during the development and progression CRPC.