SAFB Antibodies

Scaffold attachment factor B1 (SAFB)

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly stage to enable the formation of a'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription.

When associated with RBMX, binds to and stimulates transcription in the SREBF1 promoter (By similarity). Can inhibit cell proliferation.

Gene Information

Human
Gene Name:	SAFB
Uniprot:	Q15424
Entrez:	6294

Family

Belongs to:
No superfamily

Alternative Names

glutathione S-transferase fusion protein; HAP; heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein; HETDKFZp779C1727; Hsp27 ERE-TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAF-B; SAF-B1; SAFB1SAB-B1; scaffold attachment factor B; scaffold attachment factor B1

Molecular Weight

Mass (kDA):
102.642 kDA

Genomic Context

Human
Location:	19p13.3
Sequence:	19; NC_000019.10 (5623081..5668478)

Tissue Specificity

Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.

Subcellular Localizations

Nucleus.

Diseases

• Malignant Neoplasms
• Neoplasms
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Cell Transformation, Neoplastic
• Mutation, Out-of-frame
• Neoplasms, Experimental
• Werner Syndrome
• Bone Neoplasms
• Aneuploidy
• Immediate Hypersensitivity

• Mast-cell Sarcoma
• Osteosarcoma
• Congenital Heart Disease
• Neoplasms, Hormone-dependent
• Posttransfusion Purpura
• Lymphoma

Interacting Proteins

• FUS
• MAP1LC3B
• SAFB2
• Srek1
• TP53

Pathways

• Localization
• Cell Proliferation
• Rna Splicing
• Cell Growth
• Rna Processing
• Dna Repair
• Complement-dependent Cytotoxicity
• Cell Cycle
• Chromatin Organization
• Histone Deacetylation
• Induction Of Apoptosis
• Aging
• Anaphylaxis

• Hypersensitivity
• Nuclear Export
• Outflow Tract Morphogenesis
• Drug Resistance
• Sensitization
• Mast Cell Proliferation
• Interphase

PTMs

• Phosphorylation
• Sumoylation

• Deacetylation
• Cleavage

• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SAFB

Best Uses of the SAFB Marker by Steven Boster

Steven Boster is the person to look for high-affinity prima antibodies. This article will tell you about his career and current products. Steven Boster is a name you may not be familiar with. Here are some facts about him and the reasons he created the SAFB Marker. What is SAFB exactly? And how does it work? Let's examine these questions, and more.

Steven Boster

Steven Boster's Best Uses of the SAFB Marker reveals the diverse backgrounds of the authors in agricultural science. The contributors include Dean R. Bader, a junior at the University of Kansas in veterinary medicine, and Charles G. Bailey of Topeka. Robert A. Baird, Ohio, and Carol A. Bowman, Salina. Several chapters also address topics such as economics, history, and the importance of science in the community.

Boster was a farmer as well as a farmer. Boster was a farmer who raised chickens, corn and even a garden. Later, his interest in horticulture developed. He was able, as a result of his research, to help develop the science behind raising poultry. He cultivated more than 40 varieties of wheat during his time at the University of Illinois.

The book's author biography provides details about the lives of the contributors. Grubb, for example, is a Ellsworth resident. Haden, on the other hand, is a member Sigma Tau Cor Sec as well as Steel Ring Treas. Steven Boster Best uses of the SAFB Marker by Steven Boster are also members Phi Eta Sigma. It is the national honor society of agricultural engineers.

"In this volume, we have shared the stories and experiences of five students: Bradley D. James R. Salina, Gary L., and Gary L. all juniors at Hugoton. Marlene K. Morgan, a junior at Manhattan High School, is the author's mother. Sheldon L. Murphy has just finished his sophomore year at the University of Kansas. He lives in Wichita (Kan).

Primary antibodies of high-affinity

Boster Bio's rabbit antibodies are more specific than commercially available ones. They are made with Boster Bio's proprietary plasma cellular discovery platform. This platform generates the highest affinity clones that can be used to treat Diagnostics & Therapeutics goals. This technology screens individual cells after they have been treated with proprietary chemical to prevent B cells secreting antibodies. However, it retains them on the cell surface. Flurochrome-conjugated antigens are then incubated with splenocytes and plasma cells.

Steven Boster created his first product in 1993. Boster Bio expanded into several areas, including immunohistochemistry. They produced hundreds of primary antibody products. In the late nineties, Boster Bio had grown to be the largest Chinese antibody catalog. This led to the creation of PicoKine (tm), Boster Bio’s proprietary ELISA platform. It also uses proprietary trade secrets to deliver high-affinity ELISA kits.

Boster Bio begins by isolating animal spleens cells for hybridoma fusion. Titration ELISA is then performed. The ELISA screen then screens for positive supernatants. Subcloning is then done until all wells are growing at the same rate. Subcloned antibodies are further tested for bioassay application. The final step to develop monoclonal antibody is affinity chromatography. This involves the isolation of antibodies from a host serum, egg, or other source.

Enzyme-linked immunosorbent tests use both primary and secondary antibodies. These antibodies are used to detect antigens in samples and perform other tests. Western Blotting, immunohistochemistry, immunofluorescence, and proteomics are among the immunoassays that use antibodies to detect specific proteins. Other techniques include flow cytometry, which employs a specific cell line for a particular cell type.

Current products

The SAFB protein family contains nuclear proteins with diverse functions. They bind with DNA and RNA. They were originally known as scaffold association factors. These proteins are believed to prefer AT-rich regions of DNA, insoluble arrays of filaments, and the nuclear matrix. Recent studies have shown that SAFB is able to bind to many proteins and other transcription factors. One of the most striking features of SAFB is its potential to modify KRAS4B localization.

The SAFB family contains several RNA-binding proteins, including two that are highly conserved in human breast cancer. These proteins also have the ability to package into extracellular vesicles. Recently, SAFB was reported to be both a tumor suppressor as well as an estrogen receptor corepressor. Although they have many functions, these proteins share some common features. They are almost identical in their amino acid sequences to other proteins, which means that SAFB is an integral part many biological processes.

SAFB1 and SAFB2 have been shown to co-localize with nuclear speckles, in addition to binding XIST. These proteins could have an indirect or direct role to maintain chromatin structure. The SAP domain could also play a role with SAFB function. Some of these studies suggest that SAFB may bind to noncoding RNA, such SatIII repeat RNA.

Reduced levels GTP-loaded Ras can result from the loss of SAFB. This is why the SAFB/RAP1B-RAP1B combination is necessary for the binding of these proteins and the membrane. FNTA and SAFB establish an epistatic relationship in small GTPase membrane association. FNTA expression can also influence the effects of SAFB deficiencies. This could explain how SAFB deficit can interfere with alternative prenylation.

Future plans

The SAFB Marker was found to down-regulate L1 RNA and long genes. Fig. 7 shows the top 20 genes affected by SAFB. 7i. Genes that are not affected by MILs are generally unaffected. Future plans for SAFB Marker are to develop a diagnostic test targeting this gene and a complementary anti-viral drug. In addition to advancing drug development and disease prevention, the SAFB Marker is useful for clinical trials.

The SAFB Marker binds Super-MIL in the PSMA1 gene, and L1HS and m6A-labeled L1 RNA. It does not bind non-L1 RNA. The protein recognizes these RNA sequences and inhibits their expression. SAFB does however not act as a receptor or bind to otherRNAs. The next step will be to validate the marker for clinical use in humans.

Researchers can investigate the global regulatory role MILs in a cell by over-activating MILs. TT-Seq was performed in cells that were depleted of either SAFB or SAFB + B2. TBIs of intronic L1s with host genes were calculated. Knockdown of SAFB reduced TBIs of Super-MILs and increased their transcriptional blocking of host genes.