RGS14 Antibodies

Regulator of G-protein signaling 14 (RGS14)

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form.

Besides, modulates signal transduction via G protein alpha subunits by functioning as a GDP-dissociation inhibitor (GDI). Has GDI activity on G(I) alpha subunits GNAI1 and GNAI3, but not on GNAI2 and G(o) alpha subunit GNAO1.

Gene Information

Human
Gene Name:	RGS14
Uniprot:	O43566
Entrez:	10636

Family

Belongs to:
No superfamily

Alternative Names

regulator of G-protein signaling 14; regulator of G-protein signalling 14

Molecular Weight

Mass (kDA):
61.447 kDA

Genomic Context

Human
Location:	5q35.3
Sequence:	5; NC_000005.10 (177357672..177372601)

Subcellular Localizations

Nucleus. Nucleus, PML body. Cytoplasm. Membrane. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cell projection, dendrite. Cell projection, dendritic spine. Cell junction, synapse, postsynaptic density. Associates with the perinuclear sheaths of microtubules (MTs) surrounding the pronuclei, prior to segregating to the anastral mitotic apparatus and subsequently the barrel-shaped cytoplasmic bridge between the nascent nuclei of the emerging 2-cell embryo. Localizes to a perinuclear compa

Diseases

• Nervousness
• Malignant Neoplasms
• Cholangiocarcinoma
• Leukoencephalopathy, Progressive Multifocal
• Lymphomatoid Granulomatosis
• Neurodegenerative Disorders
• Colonic Neoplasms
• Malignant Tumor Of Colon
• Carcinogenesis
• Parkinson Disease
• Cardiovascular Diseases
• Acute Promyelocytic Leukemia
• Pain

• Post-traumatic Stress Disorder
• Pertussis
• Gliosis
• Lung Neoplasms
• Nerve Degeneration
• Cognition Disorders
• Atrophy

Interacting Proteins

• GNAI3
• TINF2

Pathways

• Localization
• Cell Division
• Mitosis
• Cell Growth
• Asymmetric Cell Division
• Cell Activation
• Cell Migration
• Interphase
• Lymphocyte Migration
• Spindle Organization
• Transport
• Protein Phosphorylation
• B Cell Activation

• Nuclear Export
• Cytokinesis
• Response To Morphine
• Negative Regulation Of Cell Activation
• Chemotaxis
• Long-term Memory
• Microtubule Polymerization

PTMs

• Phosphorylation
• Oxidation

• Prenylation
• Myristoylation

Research Areas

• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RGS14

Boster Bio - Best Uses Of The RGS14 Marker

BosterBio's Anti-RGS14 Antibody Antibody requires that you have read the Validation and Applications thoroughly before using it. We will also discuss their Cross-Reactivity against human RGS14. Boster's incentive scheme to reward early reviewsers with product credits will also be discussed. This program is open to all scientists. Read on to learn more about the RGS14 marker.

Validation

Validation of RGS14 was done in rodents as well as humans. The antibody was generated from a human peptide sequence, and recognized both rodent and monkey RGS14. Thus, it was found to be a suitable marker for LTP induction and expression in human GABAergic axons. The results demonstrated that LTP induction can also be prevented by targeting RGS14.

The RGS14 gene, which is highly expressed in the hippocampal area's postsynaptic regions, is enriched. It is found in the spines as well as dendrites of the CA2 pyramidal cells. In mouse models, knockout mice showed improved spatial learning and robust localization of the LTP in the region of CA2. The RGS14 gene is crucial for the limitation synaptic plasticity in hippocampal based learning.

The full-length RGS14 protein has been detected in both mouse striatal lysate and fixed brain tissue. Further studies are needed to understand the chemical phenotypes and immunoreactive cells of RGS14. The findings also indicate that the RGS14 marker can be used to identify synaptic networks in both mouse and human brain. Its detection is highly sensitive, and it allows researchers to perform quantitative MRI and fMRI studies.

Applications

New techniques have made it possible to study cellular function. This has brought new interest in RGS14. RGS14, which is synthesized and targeted to membranes by scientists, can be transported throughout the cell with many potential applications. Although its role within the nucleus is still unclear, it may play a role as a regulator of gene expression and subsequent synapse formation.

RGS14 has been shown to play an important role in recruiting genes to chromocenters. Gene silencing occurs when genes are recruited to compact chromocenters. There, loose chromatin moves into the interchromatin compartment where transcriptional activity takes effect. RGS14 may play an endogenous role in gene regulation, silencing, and gene regulation.

Researchers from the Max Planck Florida Institute for Neuroscience and Emory University discovered a new function for RGS14. According to Paul Evans, a Post-doctoral Researcher at the Ryohei Yasuda Lab at the Florida Institute for Neuroscience, RGS14 is a specialized scaffolding protein with unique domains.

RGS14 is located in the cell's subnuclear area, but it is transported to nucleus by the exact same route. RGS14 is enriched in NPC-rich intranuclear channels, which are considered highly specialized membrane subdomains of the ER. These channels are believed to improve macromolecular transportation. RGS14-mutant mice can be used to study the behavior of these cells in vivo.

RGS14 was detected by cells that had GAPDH as cytoplasmic markers. It colocalized also with intranuclear lamine A/C puncta as well as chromatin rich regions. Its presence on chromosomes may indicate transcriptional regulation or RNAsplicing. These results indicate that RGS14 plays an important role in the regulation cellular processes, such as DNA replication or transcription.

The subcellular localization of RGS14 has been studied in HeLa cells. RGS14 is most commonly found in the neuron cytoplasm. RGS14 can build up in the nucleus if it is prevented from nuclear export. GFP-RGS14 also accumulates in the nucleus after heat shock or treatment with nuclear export inhibitors. It is possible, therefore, that native RGS14 might be found in a nucleus during synaptic or neuronal stimulation.

Cross-reactivity to human RGS14

Cross-reactivity in mice with human RGS14 is possible for many reasons. These include its ability recognize a wide range proteins, the possibility for cross-reactivity, as well as its potential therapeutic value. RGS14's ability to detect cardiac hypertrophy in cultures and in vitro is another advantage. This clinical benefit could be due to RGS14's ability to regulate the signalling pathway for MEK1/2 and ERK1/2, important cardioprotective proteins.

The protein consists a RGSdomain that promotes GAP activation and a GoLocodomain that is involved GDI activity. Both domains target the G ia subclass, and RGS14 is also known to bind to Rap interacting protein. It is associated w/ centrosomes and MTs. It also colocalizes with G ia and pericentrin. Mice without RGS14 can not progress beyond the two cells stage.

Human RGS14 exists in all tissues but hematology. Snow et. Snow and colleagues identified cDNAs derived from rat brain glioma cells, which encoded a predicted 544 amino acid protein. RGS14 has high levels of expression in the brain, spleen, and other tissues. ESTs from human beings that map to RGS14 gene, chromosome 5, were also identified.

The RGS14 genome contains fifteen exons. It spans more that 16 kb. Sierra et al. Sierra et.al. This is a far more efficient way to screen mice cells for RGS14. An RGS14 antigen can be purchased to cross-react in a rat model.

RGS14 has a role in synaptic plasticity. RGS14 seems to be especially important in the CA3-CA1–DG circuit. RGS14 was also associated with learning and memory in mice. It is still not fully understood but it does play an important role in hippocampal-based Learning.