DNA endonuclease RBBP8 Antibodies

DNA endonuclease RBBP8 (RBBP8)

Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double- strand break (DSB) repair through the homologous recombination (HR) pathway. HR is limited to S and G2 phases of the cell cycle and preferentially repairs DSBs caused by replication fork collapse.

Key determinant of DSB repair pathway option, as it commits cells to HR by preventing classical non-homologous end- joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the creation of ssDNA.

Gene Information

Human
Gene Name:	RBBP8
Uniprot:	Q99708
Entrez:	5932

Family

Belongs to:
COM1/SAE2/CtIP family

Alternative Names

CtBP-interacting protein; CtIP; DNA endonuclease RBBP8; EC 3.1; RBBP-8; retinoblastoma binding protein 8; Retinoblastoma-binding protein 8; Retinoblastoma-interacting protein and myosin-like; RIMSAE2; Sporulation in the absence of SPO11 protein 2 homolog

Molecular Weight

Mass (kDA):
101.942 kDA

Genomic Context

Human
Location:	18q11.2
Sequence:	18; NC_000018.10 (22914121..23026486)

Tissue Specificity

Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level).

Subcellular Localizations

Nucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).

Diseases

• Malignant Neoplasms
• Neoplasms
• Mammary Neoplasms
• Malignant Neoplasm Of Breast
• Genomic Instability
• Retinoblastoma
• Cell Transformation, Neoplastic
• Malignant Neoplasm Of Ovary
• Ataxia
• Ovarian Neoplasm
• Ataxia Telangiectasia
• Telangiectasis

• Carcinoma
• Genotoxic Stress
• Leukemia
• Myeloid Leukemia
• Prostatic Neoplasms
• Aneurysm
• Neoplasm Metastasis

Interacting Proteins

• BRCA1
• EXD2
• EXO1
• LMO1
• LMO4

• MRE11
• NBN
• PIN1
• PSIP1
• RB1

Pathways

• Cell Cycle
• Dna Repair
• Cell Cycle Checkpoint
• Localization
• Double-strand Break Repair
• S Phase
• Hypersensitivity
• Cell Cycle Arrest
• Dna Damage Checkpoint
• Cell Proliferation
• Dna Replication
• Rna Interference
• Sensitization

• Meiosis
• Oncogenesis
• G1 Phase
• Aging
• Regulation Of Gene Expression
• Chromosome Segregation
• Reverse Transcription

PTMs

• Phosphorylation
• Cleavage
• Ubiquitination
• Sumoylation

• Reduction
• Deacetylation
• Acetylation
• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RBBP8

Best Uses Of The RBBP8 Marker

If you're interested in learning more about RBBP8 and the RBBP8 marker, read on. You will learn how to use a Boster Bio Anti–PROM1 Antibody Pinoband, as well as how to get started using the antibody. We'll talk about the best uses of this antibody. We'll also be discussing how it works together with Boster’s genome endonuclease as well as its best uses.

RBBP8

Boster Bio has created a polyclonal anti-RBBP8 DNA endonuclease antibody. This antibody reacts to both human and mouse DNA samples. It comes in PBS with 0.02% of sodium azide. It has been raised against a synthetic peptide containing 14 amino acid that binds with RBBP8. If you are interested in using this antibody to determine whether a certain gene is present in your sample, you can purchase a blocking peptide.

DNA endonuclease: RBBP8

Boster Bio DNA endonucleases RBBP8 (and RNAse B) recognize DNA sequence polymorphisms. Polymorphisms of DNA sequences are known to alter the patterns of restriction endonuclease digests. Southern blot analysis is a method used to determine polymorphisms in a DNA sequence. The Boster Bio markers are derived from yeast and contain the MRE11 and Sae2 homologs.

Anti-PROM1Antibody Picoband

The RBBP8 marker is an epitope of a DNA endonuclease. The antibody reacts with the protein in human, mouse, or rat cells. It is supplied in PBS containing 0.02% sodium azide. The antibody is raised against a synthetic propeptide of 14 amino acid. You can buy the peptide separately to block this protein.

Boster Bio Anti-PROM1 Antibody Picoband

This product is validated for use in Flow Cytometry, Immunohistochemistry, and ELISA applications. It reacts well with mouse and rodent cells and is stored at 20°C. It comes in a plate of 96 wells with 4 mg Trehalose, and 0.2m Na2HPO4. The product can be used for a variety of applications, including the detection of inflammatory mediators in cell culture.

IHC

The RBBP8 gene is a protein found in the cell's nucleus. It plays a role in DNA replication. The protein interacts also with BRCA1 & the MRE11/RAD50 compound to assist in DNA damage detection and signaling. It is useful in IHC, colorimetric staining, and other tumors.

CtIP/RBBP8 levels are associated with the response of tumors to chemotherapy. Those with normal levels of the protein respond better to combined radiotherapy, chemotherapy, or hormone therapy. Low levels of CtIP/RBBP8 are more aggressive and more likely proliferate. RBBP8 expression is associated with a higher rate of survival.

These results are encouraging but more research is needed to confirm them. Tamoxifen might not be as effective if tumors express low levels of CtIP/RBBP8. These patients may have low levels due to a mutation of RB1.

ICC

The RBBP8 is a methylated variant of a specific sequence of DNA. When combined with a high-sensitivity urine pirosequencing test, methylation can increase the detection limit by three to fivefold. This pyrosequencing tool has great diagnostic potential. The cutoff value of the test is determined using receiver operating characteristics (ROC) curve analysis. The cutoff level for RBBP8 methylation (in urine) was 0.9. There were no significant differences between benign and non-malignant controls. However, RBBP8 methylation was higher in samples taken from BLCA patients.

A large panel of cancer- and normal-cell lines was used to examine the RBBP8 methylation status. Low levels of RBBP8 methylation promoter were found in cervical squamous-cell carcinoma, head and throat, and lung. The methylation frequency of RBBP8 in lung and cervical cancer was 1.3% and 1.1%, respectively. This methylation level was also found in cervical squamous cells carcinoma, prostate cancer, and esophageal cancer.

The qPCR analysis of the RBBP8 promoter indicated a clear reexpression of the RBBP8 gene. The RT4 cells methylate and the J82 cell unmethylate the promoter. The PCR products could be visualized on 2% agarose with ethidiumbromide, UV light and the EpiTect (r) PCR Control DNA set.