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- Table of Contents
1 Citations
Facts about Programmed cell death protein 1.
Potential cell death inducer, in association with other elements. .
Human | |
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Gene Name: | PDCD1 |
Uniprot: | Q15116 |
Entrez: | 5133 |
Belongs to: |
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No superfamily |
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2
Mass (kDA):
31.647 kDA
Human | |
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Location: | 2q37.3 |
Sequence: | 2; NC_000002.12 (241849881..241858908, complement) |
Cell membrane; Single-pass type I membrane protein.
You're in the right spot If you're looking to purchase an Anti-PD1/PDCD1 marker. This article will give you information about Steven Boster and Boster Bio as well as the numerous products that Boster Bio offers. We'll also demonstrate how to verify Boster antibodies by using WB and IHC. Then, we'll go over how Boster's antibodies are validated.
You've come to the right spot if you're searching for a high-affinity, specificity antibody for Flow Cytometry and other applications. Steven Xia, a renowned histologist established Boster Bio in 1993. They have been validated for a variety of applications with their high-affinity and specificity antigen. Boster Bio guarantees their products with a 100% guarantee because of the superior quality of their products. Additionally the Boster Quality Guarantee covers all their products. Additionally, they constantly expand their product line every month.
If you've been searching for the background of Steve Boster, you've come to the right location. There are numerous public records that pertain to Steve Boster. These include his address, current and previous as well as phone numbers, email addresses, and phone numbers. You can also search for his known relatives and other people who are connected to Steve Boster. To learn more about him, filter the results by age and state. When you look up Steve Boster, the details will be displayed.
Boster Biologicals' products for the detection of the PDCD1 marker can be used in research in immunology and are confirmed using microbial Toll like receptor ligands and inflammation-related Cytokines. Boster also provides product credits for the first reviewers. Scientists from all over the world make use of the company's products. Boster offers a Boster Quality Warranty to ensure the quality of.
Anti-PD1 antibodies are among the most powerful tools for measuring the expression level of this marker in cells. BosterBio's Anti-PD1/PDCD1 antibody (RP1039) reacts with human and mouse PDCD1 protein. The antibody is stable at temperatures of 4 degrees Celsius and -20degC for a year. The product contains 5mg BSA Recombinant human PDCD1 protein and blocking peptide.
There are a variety of ways to establish the authenticity of an IHC or WB antibody. One of the most important is to conduct antibody validation for WB. This ensures the accuracy of WB or IHC analyses and proves that the antibody recognizes its target. The online database Antibody-pedia offers guidelines for testing antibodies. To obtain approval of data, you need to submit original experimental data, in addition to commercial antibodies.
Two to five companies demonstrate moderate validation of their antibodies but do not provide detailed descriptions of their procedures. Their datasheets provide background information about the immunogen and target. Three of these companies gave the entire sequence of the sequence, and a fourth one only identified the region that surrounds the phosphorylation location. Three companies provided suggested applications and starting dilutions. Each of them provided at least one example of an antibody that could successfully identify its target in one of these applications.
The Met antibody that targets growth factors for hepatocytes is another example of an unvalidated anti-body. Five different antibodies were validated. One of the commercial antibodies was reproducible while the other four weren't. The staining patterns of two different batches of Met antibody were not identical, and two others were non-specific and nuclear. Similar situations occurred with the IHC analysis of VEGF. These are examples of low-level validation.
Next, validate the antibody by using the appropriate test or context. Validation of WB is especially important for antibodies used in Western blotting. Validation of antibodies can be accomplished by using immunohistochemistry or Western Blot. While Western Blotting is often the first step in evaluating new antibodies, it is not a standard method for determining whether antibodies recognize their epitope in the original conformation. Tissue fixation can create additional complications. Tissue fixation could cause additional complications.
When validating antibodies using WB, IHC, it is important to select the right negative and positive controls to ensure the correct results. Positive controls should express the target protein, while negative controls shouldn't. This way, researchers can detect possible sources of error before they affect their results. Positive controls also provide information about the immunoblotting procedure. Positive results from positive controls indicate that the protocol used to detect antibodies is effective and gives credence for other tests. Negative results from positive controls are a sign that the Ab has a weak binding to the protein of interest.
PMID: 7851902 by Shinohara T., et al. Structure and chromosomal localization of the human PD-1 gene (PDCD1).
PMID: 9332365 by Finger L.R., et al. The human PD-1 gene: complete cDNA, genomic organization, and developmentally regulated expression in B cell progenitors.
*More publications can be found for each product on its corresponding product page