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- Table of Contents
Facts about 26S proteasome non-ATPase regulatory subunit 8.
Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. .
Human | |
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Gene Name: | PSMD8 |
Uniprot: | P48556 |
Entrez: | 5714 |
Belongs to: |
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proteasome subunit S14 family |
26S proteasome non-ATPase regulatory subunit 8; 26S proteasome regulatory subunit p31; HIP6,26S proteasome regulatory subunit S14; HYPF; MGC1660; Nin1p; p3126S proteasome regulatory subunit RPN12; proteasome (prosome, macropain) 26S subunit, non-ATPase, 8; Rpn12; S14
Mass (kDA):
39.612 kDA
Human | |
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Location: | 19q13.2 |
Sequence: | 19; NC_000019.10 (38374550..38383824) |
Boster Bio's PSMD8 Marker is a highly specific antibody. It has been validated across multiple platforms using known positive and negative samples. Its high affinity and specificity makes it an excellent choice for many applications in cell biology, tissue culture, and virology. Boster also credits the first reviewers with a free antibody sample. Boster also rewards all scientists around the world for their first review and product credit.
Recent research demonstrates that ICCRNAseq can be used for identification of cells with high expressions of the PSMD8 marker. The researchers used data taken from MCF-7 cells. This cell line expresses high levels, while other cells have lower expression. The findings were cross-validated in MCF-7 cells. These analyses could be extended and extended to other cell groups by investigating gene expression profiles using different biomarkers. As such, ICC-RNAseq has the potential to help investigators understand the molecular mechanisms of cell population specific disease.
The authors used an ICC-based targeted approach to characterize single cells of MCF-7 cancer cells. This combined routine pathology, immunocytochemistry and LCM. They demonstrated that loss or reduction in PR expression leads to a shift on the transcriptional profile, compensatory intracellular signaling and metabolic modulation. As supplementary materials, the ICCRNAseq dataset was provided.
The PSMD8 marker was used to study the role and expression of estrogen and progesterone receptors in breast cancer. They found that MCF-7 cancer cells expressed PR in different ways than normal breast tissue. These findings could have implications for breast cancer patients. A study published online in the journal Molecular Biology of Cancer demonstrated that breast cancer patients with a loss of estrogen receptors or PR in the MCF-7 luminal line of cells was associated with lower survival rates.
PSMD8, a cell surface receptor, is expressed on immune cells. It is found on all types of cells, including monocytes and dendritic cell, as well as hematopoietic and splenocytes. Boster Bio provides antibodies that react to this cell surface receptor. Boster Bio antibodies have been well validated in immunohistochemistry, Western Blotting, and ELISA.
Fluorescent-conjugated antibodies can be used to analyze cells directly by flow cytometry. Direct immunofluorescence stains, on the other side, involves incubating cells directly with antibodies conjugated to fluorophores. This approach eliminates the possibility of non-specific binding, which can occur when secondary antibodies fail to penetrate cells. In contrast, indirect staining uses fluorescent secondary antibodies to identify the target protein.
PMID: 7621825 by Kominami K., et al. Nin1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae.
PMID: 1317798 by Kanayama H.O., et al. Demonstration that a human 26S proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes ATP and ubiquitin-ligated proteins by closely linked mechanisms.