Prion-like protein doppel Antibodies

Prion-like protein doppel (PRND)

Required for normal acrosome reaction and for normal male fertility (By similarity). Can bind Cu(2+) (PubMed:15218028, PubMed:20411530).

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Gene Information

Human
Gene Name:	PRND
Uniprot:	Q9UKY0
Entrez:	23627

Family

Belongs to:
prion family

Alternative Names

Prion-like protein doppel

Molecular Weight

Mass (kDA):
20.293 kDA

Genomic Context

Human
Location:	20p13
Sequence:	20; NC_000020.11 (4721909..4728460)

Tissue Specificity

Expressed in testis, in Sertoli cells, ejaculated spermatozoa and in seminal fluid (at protein level).

Subcellular Localizations

Cell membrane; Lipid-anchor, GPI-anchor.

PTMs

• Disulfide bond
• Glycoprotein

• GPI-anchor
• Lipoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PRND

Best Uses Of The PRND Marker

If you're looking for a primary antibody to the PRND Marker, Boster has the primary antibodies that you need. His antibodies have been used for decades, and are backed by research community trust. Each one of Boster's antibodies has been validated in Western Blotting, Immunohistochemistry, and ELISA. Read on to learn more about the PRND Marker and Boster Bio's products.

His research

His work on the PRND Marker has opened up new possibilities for understanding the disease. Although this gene seems not to play a major role in the pathogenesis of prion diseases, his report of polymorphisms in the PRND gene has paved the way to further investigation of the protein's role in other human diseases. The next step is to discover which polymorphisms are linked to the disease.

To find the PRND gene, researchers used a ME method. This method identifies mutations that are rare, but still important to the production of high-quality livestock. Using this approach, the researchers were able to detect a 20-bp indel within the sheep PRND gene. These indels affected a number of traits, including growth rate. Thus, this protein may prove to be a useful DNA marker for identifying high-quality individuals.

His primary antibodies

The PRND gene is a member of the prion protein family that is expressed in the gonads. In goats and mice, PRNP and SPRN are expressed constantly, although the latter has less frequent expression. PRND is a key marker for the foetal Leydig cells and may play a role in spermatogenesis. The encoded protein Doppel plays an unknown role during gonadal differentiation.

His PFGE work

The use of PFGE has increased in recent decades. This technique has become popular for the identification and molecular typing of bacteria and other pathogens. These methods can detect about ninety percent of a bacterial genome. Researchers have also been using PFGE to monitor global genetic changes in bacteria, such as the increase of antibiotic-resistant genes. This study could help doctors determine how to better protect patients and staff from the spread of infectious diseases.

PFGE is used for genetic analysis of bacterial genomes when classical genetic techniques are ineffective. It produces large parts of the genome through the action of restriction enzymes. These large parts can then be plotted to create genome structural maps. These maps can be used to perform genetic mapping by analyzing cloned genes. This technology is expensive and time-consuming, but it is an effective way to identify bacteria and pathogens that cause foodborne outbreaks.

DNA fragments of various sizes can be separated using pulsed-field gel electrophoresis (PFGE). Currently, PFGE can separate DNA fragments up to 20 kb. It has also expanded the size range of electrophoretic DNA separations. Hence, it has become a common method for molecular analysis. It is useful for gene mapping, and was one of the main catalysts for the development of large-insert cloning systems.

The technique is highly sensitive in determining the species and the virulence factors of a disease. It can also be used for the evaluation of existing microorganisms in soil, or to prepare vaccines. PFGE is widely regarded as a highly effective method for controlling diseases and other infections in recent decades. So how does it work? Find out how Boster's PFGE can help you!