Protein phosphatase 1E Antibodies

Protein phosphatase 1E (PPM1E)

Protein phosphatase that inactivates multifunctional CaM kinases like CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK.

May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2 (By similarity).

Gene Information

Human
Gene Name:	PPM1E
Uniprot:	Q8WY54
Entrez:	22843

Family

Belongs to:
PP2C family

Alternative Names

ca(2+)/calmodulin-dependent protein kinase phosphatase N; CAMKN; CaMKP-N; caMKP-nucleus; DKFZp781F1422; KIAA1072; nuclear calmodulin-dependent protein kinase phosphatase; partner of PIX 1; partner of PIXA; partner of PIX-alpha; POPX1; PP2CH; protein phosphatase 1E (PP2C domain containing); protein phosphatase 1E; protein phosphatase, Mg2+/Mn2+ dependent, 1E

Molecular Weight

Mass (kDA):
83.952 kDA

Genomic Context

Human
Location:	17q22
Sequence:	17; NC_000017.11 (58755854..58985617)

Subcellular Localizations

Nucleus. Cytoplasm. A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full-length, minor form, is found in the nucleus.

Diseases

• Malignant Neoplasm Of Breast
• Malignant Neoplasms
• Neoplasms
• Mammary Neoplasms
• Reperfusion Injury
• Schizophrenia
• Myeloproliferative Disease
• Myeloid Leukemia, Chronic
• Diabetes Mellitus
• Hepatomegaly
• Mental Disorders

• Mulibrey Nanism
• Diabetes Mellitus, Non-insulin-dependent
• Myeloid Leukemia
• Cerebrovascular Accident
• Leukemia
• Dwarfism

Pathways

• Translation
• Gene Silencing
• Aging
• Histone Acetylation

PTMs

• Phosphorylation

• Dephosphorylation

• Acetylation

Research Areas

• Protein Phosphatase

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PPM1E

Best Uses Of The PPM1E Marker

The PPM1E protein marker is an extremely useful tool for protein research. It allows researchers to measure the protein transfer efficiency by membrane staining. This marker can also be used in immunohistochemistry, Western blotting, and ELISA. The antibody has been extensively tested for its use in biological research. You can order antibodies by choosing the number that you require from the following list.

Protein transfer efficiency by membrane staining

Boster Bio's new marker, PPM1E, increases protein transfer efficiency via membrane stain. The PPM1E label is compatible with most membrane-based protein transfer systems, making it an ideal choice for use in a wide range of applications. Boster Bio developed the marker, which recognizes a wide variety proteins, even those from eukaryotes. Scientists can identify any protein by using the marker's PPM1E labeling ability.

Boster Bio's PPM1E membrane staining kit uses a reversible membrane proteinstain that can be used to measure transfer efficiency and image total protein patterns. MemCode staining is an efficient, quick and highly sensitive option to amido staining. The PPM1E marker allows the detection of transferred protein using just a few drops MemCode on membranes. Its rapid detection means it can be used for many downstream purposes.

Electroblotting is where the target protein of interest is electroblotted on an electroblot transfer membrane. The transferred protein will be visible as black bands on a background of gray. To increase the membrane's sensitivity, wash it three more times with 1% KOH or phosphate buffered saline. Place the blot-transfer membrane in a plastic container. For five minutes, repeat these steps three more times.

The gel is sandwiched to a sponge between a membrane and a material. The sandwich is then clamped in order to remove air bubbles. Gels that are too thin may not transfer well. The gel and the membrane must be the exact same size for maximum transfer efficiency. The membrane and the gel should not be overlapping. The gel could clog your membrane and stop current flow through the gel.

Film about autoradiography

Boster offers a wide range of primary antibodies that have been validated on immunohistochemistry, Western blotting, and ELISA. These antibodies are highly specific against this marker and are often cited in the scientific community. Boster has antibodies that can be used to identify tumor-specific mutations. Here are some examples of some of the most sought-after tumor-specific antibody:

ECL chemiluminescent detection system

The Clarity Max Chemiluminescent Detection Systems are compatible all horseradish peroxidase conjugates and are suitable to be used in western blot applications. The Clarity Max detection systems are ideal for sensitive detections of small amounts. The Clarity Max has excellent sensitivity, reliability, and can be used to replace more expensive products. They also offer comparable performance to other ECL sub-substrates. They can detect HRP using photographic methods with high sensitivity.

Clarity Max ECL substrate has a key characteristic: it has a higher sensitivity. This substrate allows detection of low levels of target protein, even in femtogram amounts. The Clarity Max ECL substrate has an approximate 24-hour long-term life span. Bio-Rad Clarity max ECL substrates extend the detection time and allow for re-imaging with no loss of signal intensity.

The ECL Plex Western Blotting System can detect single and multiple proteins. The membranes use low-fluorescent PVDF/nitrocellulose. The ECL membranes allow for many different types of stripping or reprobing. ECL Plex Western Blotting System can also be used for proteins of different sizes. This system is suitable for immunoassay as well as western blot applications.

For the detection and analysis of antibodies in membranes, a high-salt buffer with 10 mMTris-Tris pH 7.4 0.5 M NaCl and 0.1%Tween-20 was used. After the membrane was washed three times with TBS-0.1% Tween, the signals were visualized using the Tanon 5200 system. The specific band was detected as 62KD. This is slightly smaller than expected.

DAB chromogenic detection system

Boster Bio's DAB color detection system is designed for quick and easy detection of apoptotic tissues in a variety bio samples. It can be used on a variety of samples including blood and tissues. The system includes a 10-X DAB solution and a DAB reagent kit. DAB can also be purchased in a crystalline form.

Uses of the PPM1E marker

The PPM1E genome encodes a phosphoprotein, AMP activated protein kinase phosphatase. The PPM1E gene plays an important role for p53-dependent tumor suppression. It also inhibits FZD7 inhibition and doxorubicin resistant. PPM1E siRNA are available in AAV, lentivirus, and oligo formats. They can be used to knockdown PPM1E gene activity in a wide range of host cells. PPM1E siRNA can be used for research purposes only and is not intended for diagnostic or therapeutic purposes.

Recent research has shown that the PPM1E marker is a viable tumor suppressor. Studies on glioma cells have shown that overexpression of PPM1E inhibits the proliferation of GC cells and slows their growth. It is also a useful target for therapeutics to inhibit apoptosis. Further, it has also been reported that PPM1E can be used to target cancer cells in glioma and metastatic tumors.

Many studies have focused on thyroid cancer cells. However, the PPM1E protein marker has been implicated in other types of thyroid tumors. Recent studies revealed that a single sh LINC01087-treated group had a decreased level of the PPM1E protein. Another study found that sh-LINC01087 inhibits thyroid cancer by reducing the levels of the miR-135a-5p gene. The study also showed that PPM1E was related to cancer by miR136a-5p, and miR-135a-5p. It was also negatively associated with miR-135a-5p (which are important cancer-related molecules)