This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about DNA polymerase alpha subunit B.
.
Mouse | |
---|---|
Gene Name: | Pola2 |
Uniprot: | P33611 |
Entrez: | 18969 |
Belongs to: |
---|
DNA polymerase alpha subunit B family |
DNA polymerase alpha 70 kDa subunit; DNA polymerase alpha subunit B; FLJ21662; FLJ37250; polymerase (DNA directed), alpha 2 (70kD subunit); polymerase (DNA-directed), alpha (70kD)
Mass (kDA):
66.214 kDA
Mouse | |
---|---|
Location: | 19|19 A |
Sequence: | 19; |
We will be discussing the main antibodies to the POLA2 marker, as well as the history and life of Steven Boster. We will also discuss its uses in research and biotechnology. The best application of the POLA2 markers is in the development anti-senseRNA products. This marker is available worldwide. It can also be used to screen primary antibodies for bacteria and viruses. It is a simple and effective tool in molecular biology.
Recent research has shown that POLA2 encodes the DNA polymerase beta subunit 2. Despite the lack evidence for its exact role, POLA2 appears to be linked with the cellular DNA replication machinery and play an essential part during the early stages in chromosomal genetic DNA replication. To confirm this hypothesis, Boster's primary antibodies and the POLA2 marker have been validated by Western Blotting, Immunohistochemistry, and ELISA.
The primary antibody is an immuneglobulin that recognizes specific biomolecules. It is made using an animal as a source of host. It can be produced in a crude antiserum or an antigen-purified formulation, or as fluorescent dyes. Some primary antibodies may also be labeled using biotin. In many instances, the primary antibody can also come from an alternate source.
Absorption control can cause a number of problems. Absorption controls prevent antibodies binding to all proteins. They do not prohibit primary antibodies from binding other proteins. Therefore, absorption controls can give false negative results, since an antibody is specific to only one antigen. Another difficulty is that some epitopes on the antigen may bind to all antigens.
It is also possible to do fluorescent mIHC. It combines primary antibodies from different species and fluorescent dyes. This method is much more common than chromogenic microIHC. It uses fluorescent secondary antibody to increase amplification. However, the method is limited to three markers because of the filter sets available. The use of fluorescent mIHC requires the use of primary antibodies raised in different species and isotypes.
You are here if you are looking for Steve Boster's past. You can now search his public records and discover his past addresses, mobile phone numbers, email addresses, and even known relatives. To see which records are related to Steve Boster, you can also find out his age and where he lives. Steven Boster's name is associated with many historical landmarks. The list below is not complete, but it should give you an idea of how much you can find out about this man.
Steve Boster died June 26, 2022. He was the youngest son of Evelyn Meier Boster and James Meier Boster. He had worked as a sales manager for many years, and he was also a member of Concordia Hall in Staunton, VA. His family includes 2 Daughters - Natosha Peck and Crystal Boster - as well as 6 Grandchildren. His siblings include his brothers Jack Boster, and sisters Kimberly and Tammy. Steve's nieces-and-nieces are also surviving.
The POLA2 Marker is a great way to explore DNA damage and repair. Researchers have shown that POLA2 expression reduces cellular survival after DSBs, a type of DNA damage, and it also has the ability to enhance sensitivity to PARP1 inhibitors, a common cause of defective HR repair pathways. In addition, POLA2 loss has been shown to increase sensitivity to IR, a hallmark of DSB repair failure, which can involve either the HR or NHEJ pathways.
PCR was used to amplify genomic DNA for the POLA2 gene. After amplification of the DNA, it was blunt-ended with a DNA Blunting Kit. The results of the PCR confirmed that POLA2 was associated with embryo lethality. The PolA2 marker was found to co-segregate with the phenotype in an F2 population. The POLA2 genes are located on chromosome 11,q13.1.
POLA2 gene analysis is a tool that can help monitor drug responses. POLA2 levels being directly correlated to Erlotinib resistant could be used as a biomarker for Erlotinib-resistant POLA2. POLA2 could be used in proteomics applications, as NGS is a widely used tool in molecular oncology. POLA2 could also be used as a biomarker to target drugs that are resistant to TKIs.
PMID: 8463324 by Miyazawa H., et al. Molecular cloning of the cDNAs for the four subunits of mouse DNA polymerase alpha-primase complex and their gene expression during cell proliferation and the cell cycle.