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- Table of Contents
Facts about Perilipin-3.
Human | |
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Gene Name: | PLIN3 |
Uniprot: | O60664 |
Entrez: | 10226 |
Belongs to: |
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perilipin family |
Cargo selection protein TIP47; M6PRBP1; M6PRBP1MGC2012; Mannose-6-phosphate receptor-binding protein 1; perilipin 3,47 kDa MPR-binding protein; Perilipin3; Perilipin-3; Placental protein 17,47 kDa mannose 6-phosphate receptor-binding protein; PLIN3; PP17; PP17MGC11117; tail-interacting protein, 47 kD; TIP47; TIP47mannose-6-phosphate receptor binding protein 1
Mass (kDA):
47.075 kDA
Human | |
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Location: | 19p13.3 |
Sequence: | 19; NC_000019.10 (4838341..4867667, complement) |
Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
In this article, you'll learn about the benefits of using the PLIN3 Marker. To understand the benefits, read about Overview, High affinity primary antibodies and Gene infographics. Find out more about Boster Bio's other offerings such as their DNA-methylation kit. Boster's biomarker is not suited to a specific application or species. It is applicable to researchers from all over the world Continue reading to discover some of its most common uses.
The PLIN3 marker was created in the mid-1990s as an effective tool to evaluate liposarcoma. There are many kinds of sarcomas that the PLIN3 gene is found in, including cutaneous, leiomyosarcomas and rhabdomyosarcom. PLIN3 is found on more than half of LDs in a variety of sarcomas, such as sarcomas.
The PLIN3 marker was identified as a biomarker for the detection of cancer. It is made up of two distinct amino acid residues, one of which is a hydrophobic LDL. In electron micrographs, PLIN5 was associated with mitochondria in cardiac muscle cells. These proteins were recovered from cardiomyocyte mitochondrial fractions. However, it is still unclear what the PLIN3 marker is involved in the human cancer.
T2DM and obesity are both affected by the marker PLIN3. Obese T2DM subjects had the lowest levels of PLIN3. Future research should address the physiology of these correlations. In this regard, authors declare no conflicts of interests. DZA and AA contributed to the study's conception. DZA collected data as well as analysis and wrote the manuscript draft. OA and NMA gave critical feedback on the manuscript. All authors ratified the final version.
The signal sequence of PLIN3 marker is glycosylated, protecting it from degradation by proteinaseK. The lower band could be an incomplete degradation of full-length protein. Erg6 can be detected through western blotting using an antibody against GFP.
The overexpression of PLIN2 can result in LD accumulation in COS-7 and fibroblasts. Mice that express PLIN2 are immune to alcohol-induced steatosis and fatty liver disease. It is believed that PLIN2 controls the accumulation of lipids in the liver. It is believed to promote LD formation and helps protect TG against lipolysis. It is a crucial marker in preventing obesity and fatty liver.
Plin2 was detectable in both LDs Plin2 was detectable in both LDs, however Plin3 was not. Plin3 was nevertheless abundant after lysosomal inhibition. The PLIN3 marker is a preferred target for lysosomal degradation. The status of its phosphorylation determines whether autophagic machinery is able access LDs. More research is required to discover how Plin3 interacts with PLIN2 and PLIN3 in human liver.
As previously mentioned as previously mentioned, the PLIN3 marker can perform multiple functions. It can be used for determining the amount of sebocytes present in the body. PLIN2 is a marker of the sebaceous gland. It is extremely expressed in human mesenchymal embryonic Adipocytes that are derived from fibroblasts. It is thought to participate in the signaling process of insulin and is expressed in stellate cells storing vitamin A in the liver.
One of the difficulties of determining the ideal antibody-antigen interaction is identifying which epitopes are most likely to be bound to the antigen target. Dot immunoblot tests utilize the protein target that is immobilized to membranes of nitrocellulose, and then incubated with the antibodies of interest. For quantitative results, the concentration curve is constructed using the target and the primary antibody. A negative control is added as a standard.
The 11-mer repeats found in the PLIN3 marker resemble those in the apolipoproteins (a protein that is involved in Parkinson's Disease). Additionally, PLIN3 is predicted to have a four-helix bundle structure similar to that of the apoE. PLIN1 and 2 also have similar structures. High-affinity primary antibodies made with PLIN3 are therefore beneficial in a variety of clinical situations.
High-affinity primary antibodies are capable of recognizing two antigens in the same tissue section. The ability to visualize one antigen is not affected by the pigments used. You can make use of optical filter combinations to distinguish one antigen from another. Scientists can identify up to 61 antigens from the same tissue segment using this technique. The PLIN3 marker is not able to identify the specific antigen. This allows scientists to identify antigens using more complicated ways.
A number of studies have revealed that the overexpression of PLIN2 causes LD accumulation in fibroblasts. Mice that have PLIN2 knockout are immune to obesity caused by diet and alcohol-induced steatosis. They also have a reduced risk of liver disease. These results suggest that PLIN2 and PLIN3 are both expressed in the same tissues might play a role in compensatory actions. Further studies are required to confirm that they co-express in humans.
LDs and macrophages are closely connected and play a variety of roles in different microenvironments. Both macrophages and LDs play a part in the body's resistance to infection as well as immunity. Therefore, it is essential to understand the mechanisms behind LDs their anti-infection properties. This could be due to a high-affinity primary antibody that binds with the PLIN3 marker.
The PLIN3 gene is believed to be the source of a variety of illnesses. The five-hybrid features of the PLIN3 gene indicate that it can be associated with more than five thousand biological entities. Gene infographics help you find out more about PLIN3 genes and their function in human diseases. PLIN3 is one of the members of the PLIN gene family. It is found in tissues of all mammals including humans. Its polypeptide length is three times greater than other PLIN proteins and it is expressed in the adipose tissue. The PLIN4 gene is localized in LDs, probably because of its 11-mer repeat.
PMID: 9590177 by Diaz E., et al. TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking.
PMID: 9874244 by Than N.G., et al. Cloning and sequence analysis of cDNAs encoding human placental tissue protein 17 (PP17) variants.