PCSK1 Antibodies

Neuroendocrine convertase 1 (PCSK1)

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.

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Gene Information

Human
Gene Name:	PCSK1
Uniprot:	P29120
Entrez:	5122

Family

Belongs to:
peptidase S8 family

Alternative Names

NEC1; PCSK1; Proprotein Convertase 1

Molecular Weight

Mass (kDA):
84.152 kDA

Genomic Context

Human
Location:	5q15
Sequence:	5; NC_000005.10 (96390333..96433248, complement)

Subcellular Localizations

Cytoplasmic vesicle, secretory vesicle. Localized in the secretion granules.

Diseases

• Obesity
• Pituitary Diseases
• Neoplasms
• Diabetes Mellitus
• Vaccinia
• Diabetes Mellitus, Non-insulin-dependent
• Insulin Resistance
• Malignant Neoplasms
• Pituitary Neoplasms
• Pheochromocytoma
• Insulinoma
• Sarcoma
• Fibrosarcoma

• Pediatric Obesity
• Insulin Sensitivity
• Neoplasm Metastasis
• Malnutrition
• Malignant Neoplasm Of Breast
• Tumor Angiogenesis
• Mammary Neoplasms

Pathways

• Secretion
• Secretory Pathway
• Localization
• Transport
• Regulated Secretory Pathway
• Insulin Secretion
• Glucose Homeostasis
• Constitutive Secretory Pathway
• Cell Proliferation
• Pathogenesis
• Zymogen Activation
• Flight
• Intracellular Transport

• Lactation
• Insulin Processing
• Angiogenesis
• Proteolysis
• Eating Behavior
• Translation
• Cell Differentiation

PTMs

• Cleavage
• Amidation
• Glycosylation
• Phosphorylation

• Sulphation
• Biotinylation
• Reduction
• Acetylation

• Oxidation

Research Areas

• Golgi Apparatus Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PCSK1

Boster Bio - Best Uses Of The PCSK1 Marker

There are a lot of questions that arise when using the Boster Bio. You might be wondering how to utilize this reagent for the best results. Fortunately, Boster Bio optimization guides will provide the answers to many of these questions. By following these guides, you can increase the effectiveness of your experiments and optimize your experiments. There is always a chance that something could go wrong in any experiment. Troubleshooting guides will help you find and avoid common sources of error in your experiments.

Techniques in molecular biology

Two distinct species of PCSK1 DNA were created using a gwt based construct for the expression of the PCSK1 gene. The hybrid resulted in two distinct, but similar, fragments of 2.4 kb each. These fragments were used for analyzing the splicing PCSK1 mRNA. They did not have any effect on the maturation, secretion of mRNA or splicing.

Researchers have also discovered PCSK1 mutations and have mapped the sequence of its 5' UTR and coding region. Novel heterozygous variants have been highlighted in bold. These positions are based on the GRCh37 reference chromosome. Carriers are individuals who carry the mutation within a sequencing cohort. The ExAC consortium offers allele frequencies. While the results look promising, more work needs to be done to validate their interpretation.

To test the validity of DART-Seq using human islets, researchers carried out an experiment with islets taken from a healthy donor. The results revealed that the latter technique produced a higher median signal value for PCSK1 in single cells, while the first had a better signal in the b cells. Unsupervised clustering also confirmed that PCSK1 expression is present in both a cells and b cells.

Furthermore, they discovered that the PCSK1 gene polymorphism was strongly connected to abdominal fat content. These results support the idea that PCSK1 can be used for choosing lean broiler lineages. This method could be a valuable tool for breeding lean broiler line. The research suggests that using PCSK1 as a marker for improving pig health is an attractive option to identify lean lines.

Sample preparation

The PCSK1 marker can be identified through PCR. The method involves coating the plate with antigen, followed by adding HRP-conjugated or biotin-conjugated rea to the wells. At each step, unbound conjugates are washed off. The HRP enzyme reaction is quantified by using a TMB substrate. PCSK1 wells that contain enough TMB substrate will give off the blue color. To turn the color yellow the acidic stop solution can be added to the plate. The amount of PCSK1 present on the plate determines the intensity of the yellow colour.

A PCSK1 variant has a nucleotide swap in the intron 1. This variant is thought to alter mRNA splicing. To test the effects of this mutation, mini-genes were created from PCSK1's complete cDNA as well as the intron. The mini-genes contained the first 218bp and the last 502bp intron. Both mini-genes were the same size as the model predicted. PCSK1 variants did no harm to the maturation or release of PCSK1 mRNA.

Mice with the PCSK1N222D mutation had significantly lower levels of HDL-C and triglycerides compared to those carrying a wild-type PCSK1N222D gene. The mutation did not affect the concentration of LDL-C in humans, which is quite impressive. This result may be due to the location of the SNVs that may alter gene expression or the ultimate role of the PCSK1 marker.

Polygenic obesity is triggered when there are several PCSK1 variants. Children's studies are better at identifying the primary and secondary relationships between PCSK1 and obesity than adults. Recent studies have revealed that children are more likely to be obese than adults due the prevalent PCSK1 variants. This suggests that the PCSK1 marker could play a key role in the pathogenesis of obesity in children.

Optimization

One of the most important things to take into account when choosing a PCSK1 antibody is the method of detection. Immunohistochemistry relies on the principle that antibodies bind to antigens to detect proteins in cells. To get the most effective results, preparation of the sample is essential. The tips and tricks listed below will assist you in optimizing your experiments and get the most precise results you can get. Troubleshooting guides are also an invaluable resource when it comes to identifying and addressing potential sources of error.

Troubleshooting

There are a variety of factors that affect the PCSK1 marker's stability of mRNA, resulting in the marker having a low OD measurement. However, the most fundamental issue is the lack of any evidence about the protein's function. The PCSK1 gene can therefore be overlooked and is not of any medical significance. Find out more about PCSK1 and learn how to troubleshoot it. We also discuss the genetic background for PCSK1 deficiency.