PCBP1 Antibodies

Poly(rC)-binding protein 1 (PCBP1)

Single-stranded nucleic acid binding protein which binds preferentially to oligo dC. In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943).

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Gene Information

Human
Gene Name:	PCBP1
Uniprot:	Q15365
Entrez:	5093

Family

Belongs to:
No superfamily

Alternative Names

alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein X; hnRNP E1; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX; nucleic acid binding protein sub 2.3; Nucleic acid-binding protein SUB2.3; poly(rC) binding protein 1; poly(rC)-binding protein 1

Molecular Weight

Mass (kDA):
37.498 kDA

Genomic Context

Human
Location:	2p13.3
Sequence:	2; NC_000002.12 (70087477..70089203)

Tissue Specificity

Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.

Subcellular Localizations

Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Neuroblastoma
• Folic Acid Deficiency
• Liver Neoplasms
• Hypoxia
• Liver Carcinoma
• Malignant Paraganglionic Neoplasm
• Tissue Adhesions
• Leukemia
• Immunologic Deficiency Syndromes
• Adenocarcinoma

• Tumor Virus Infections
• Acute Erythroblastic Leukemia
• Cell Invasion
• Infective Disorder
• Malignant Neoplasm Of Prostate
• Nasopharyngeal Neoplasms
• Papillomavirus Infections

Interacting Proteins

• PAK1
• PCBP2
• PPIG
• PRPF8
• PTBP1

• PUF60
• QKI
• RBM39
• SNRPA
• THAP11

• TSC22D4
• WBP11
• ZNF830

Pathways

• Translation
• Mrna Stabilization
• Localization
• Cell Adhesion
• Cell Migration
• Cell Cycle
• Cell Proliferation
• Cell Growth
• Rna Interference
• Transport
• Virion Assembly
• Gene Silencing
• Aging

• Immune Response
• Regulation Of Gene Expression
• Tropism
• Nuclear Export
• Spermatogenesis
• Cell Cycle Arrest
• Cell Division

PTMs

• Phosphorylation
• Cleavage
• Reduction

• Hydroxylation
• Methylation
• Ribosylation

• Cysteinylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PCBP1

Boster Bio: Best Uses Of The PCBP1 Marker

Boster scientists can submit their results for applications, special samples, and species, and can receive product credits for their work. This program is open to all scientists worldwide. However, the best use of the PCBP1 marker is in research. Here are some uses of the PCBP1 marker. The most important of all is its widespread application in scientific research. Boster Bio: Best Uses Of The PCBP1 Marker

Mouse anti-HPV16 E6 (Abcam, UK. Ab70, 1:1000), mouse anti-b-actin (Boster Inc, China, 1:300)

The PCBP1 marker is expressed in nearly all human cancers. In addition to its antiviral activity, it has other roles in the immune system. It can regulate RNA-binding proteins and inhibit the expression of HPV16 E6 oncogene. In addition, it can help determine the apoptotic status of cells. The following are some of the best uses of the PCBP1 marker.

Using Western blotting, the PCBP1 marker was detected in cervical cancer tissue, SCC, NC, HPV+, and HPV-infected samples. The expression level of hnRNP E1 correlated with HPV16 E2 and E6 expression. It also correlated with the expression level of HPV16 oncogene E1.

Mouse anti-b-actin (Boster Inc, China, 1:300)

Boster Bio Anti-B-Actin (MA1115), a monoclonal antibody to the mouse beta-actin protein, detects the actin-related proteins in mouse, avian, and canine tissues. It is purified by protein G columns and has been tested for specificity. It reacts with actin-derived proteins from human, mouse, and rat.

Boster validates their antibodies on multiple platforms with known positive and negative samples. They also ensure high affinity and specificity. Boster rewards first reviewers with product credits. This reward program is open to scientists worldwide. The company is an excellent resource for antibodies and is committed to helping researchers discover their applications. For more information on Boster, visit their website. These antibodies are available online for purchase.

Mouse anti-actin (Boster Inc, China, 1:300)

The monoclonal mouse anti-actin antibody, clone C4, recognizes human, mouse, rat, and avian actin. It is suitable for detection of all types of actin, including cardiac, smooth muscle, and yeast. It is also available as a blocking peptide, which can be purchased separately. The mouse anti-actin antibody is available in multiple conjugations.

The primary antibody for this antibody was purchased from Thermo Fisher Scientific. Other antibodies used were rabbit anti-c-actin, mouse anti-bIII-tubulin, and mouse anti-actin. Primary rat islets were used as negative controls. These were transduced with pBABE-puro or phTERT-I-GTKlox.