P2X purinoceptor 4 Antibodies

P2X purinoceptor 4 (P2RX4)

Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.

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Gene Information

Human
Gene Name:	P2RX4
Uniprot:	Q99571
Entrez:	5025

Family

Belongs to:
P2X receptor family

Alternative Names

ATP receptor; ATP-gated cation channel protein; P2RX4; P2X purinoceptor 4; P2X4; P2X4P2X receptor, subunit 4; P2X4R; purinergic receptor P2X, ligand-gated ion channel, 4; Purinergic receptor; purinoceptor P2X4

Molecular Weight

Mass (kDA):
43.369 kDA

Genomic Context

Human
Location:	12q24.31
Sequence:	12; NC_000012.12 (121210096..121234106)

Subcellular Localizations

Membrane; Multi-pass membrane protein.

Diseases

• Pain
• Neuralgia
• Nerve Damage
• Nervousness
• Inflammation
• Peripheral Nerve Injuries
• Hyperalgesia, Tactile
• Peripheral Neuropathy
• Heart Failure
• Chronic Pain
• Hyperalgesia
• Hypertensive Disease

• Malignant Neoplasms
• Hypertrophy
• Hyperalgesias, Mechanical
• Brain Injuries
• Diabetes Mellitus
• Infarction
• Myocardial Infarction

Interacting Proteins

• KRTAP5-9
• NOTCH2NL
• PIH1D3

Pathways

• Localization
• Response To Atp
• Secretion
• Hypersensitivity
• Synaptic Transmission
• Endocytosis
• Transport
• Vasodilation
• Pathogenesis
• Clathrin-mediated Endocytosis
• Cell Activation
• Inflammatory Response
• Membrane Depolarization

• Reverse Transcription
• Translation
• Phagocytosis
• Bioluminescence
• Cell Proliferation
• Innervation
• Bone Resorption

PTMs

• Phosphorylation
• Biotinylation

• Cleavage
• Glycosylation

• Deglycosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/P2RX4

Boster Bio - Best Uses Of The P2RX4 Marker

If you're a novice to antibody validation or a seasoned professional, Boster Bio has all the instruments and information you require to be successful with this marker. Boster Bio offers everything you require including WB, IHC, ICC and Immunofluorescence. Here are some of the most effective uses for P2RX4 markers.

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and Immunofluorescence

Boster Bio stocks a variety of primary antibodies from rabbit, mouse, and humans for your next project. Their antibodies are extremely specific and backed by confirmed ELISA, WB, and ICC results. In addition, they offer free secondary antibodies when you buy any Boster antibody. This is a great deal given Boster's repute for quality and research.

The RICTOR gene encodes a protein that is known as Rac1. This protein belongs to the RAS family of small GTP binding proteins that integrate signals to control cells growth. Boster Bio validates all of its antibodies on WB, IHC, and ICC. Boster Bio also offers product credits that allow scientists to share positive and negative samples.

If you need to test multiple samples using one antibody The secondary antibody is an excellent option. The secondary antibody can be conjugated to a variety of secondary antibodies that allow you to double label your specimens and provide more relevant data. Secondary antibodies can also be used to determine the number of certain cell types. While they're great for research, they're not ideal for every use.

The specificity of an antibody is a crucial aspect in the quality of an IHC test. An antibody must be able of detecting enough targets to provide a positive signal. Furthermore, the secondary antibody must be able to bind to the primary antibody in a proper manner. Repeated experiments or multiple sources might be needed to detect weak signals. It is important to identify the cause of weak signals. Sometimes, an antibody has been incorrectly validated, or there were mistakes made during the slide preparation.

Primary antibodies are produced by immunizing the host animal with a specific antibody. The quality of primary antibodies is determined by their affinity and specificity. An antibody with a strong affinity will be able to recognize the antigen with ease. However, an antibody with poor specificity might not be able to select enough. It is able to precisely detect the antigen, purify it, measure and quantify the antigen target with high specificity.

The best uses for the P2RX4 marker

The P2RX4 marker is a cell surface protein expressed in macrophages. It blocks the spread of bacteria and in the vivo inflammation. Additionally, it plays a role in the activation of immune cells. The P2RX4 marker can be found in many uses. This article will outline the most effective applications for this marker in human research. This marker is a potent tool that can be used in a range of research.

The P2RX4 protein is expressed in normal kidney cells and has not been linked with kidney diseases until now. It could play a role in the regulation of blood pressure by endothelial cells. It could be a powerful tool for detecting kidney disease in the early stages. However, it's unclear if P2RX4 has any specific clinical applications. We will look at some of the most effective applications of P2RX4's protein.

Methods

In this study, we observed that P2X4 is activated during the recognition of antigen by the antigen-presenting cells , but not after stimulation by TCR/CD3. These differences could impact the downstream mechanism within the cells. Therefore, we have analyzed their colocalization in cells by using MATLAB scripts. In the majority of samples, P2RX4 was found to be colocalized with P2X7.

The antibody used to detect P2X4 HA receptor was biotin-labeled using mouse biotin-conjugated monoclonal antibody. Then, the antibody was conjugated with streptavidin-conjugated QD655. After incubating the primary antibody for 20 minutes at the room temperature then followed by 5 minutes of QD incubation. The P2X4-HA receptor was then imaged.