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- Table of Contents
Facts about Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial.
Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate. .
Human | |
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Gene Name: | OXCT1 |
Uniprot: | P55809 |
Entrez: | 5019 |
Belongs to: |
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3-oxoacid CoA-transferase family |
3-oxoacid CoA transferase 1,3-oxoacid CoA transferase; EC 2.8.3; EC 2.8.3.5; SCOTmitochondrial
Mass (kDA):
56.158 kDA
Human | |
---|---|
Location: | 5p13.1 |
Sequence: | 5; NC_000005.10 (41730065..41870535, complement) |
Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
Mitochondrion matrix.
Flow cytometry is used to analyze cells and particles. There are many applications. BosterBio uses both monoclonal, and high-affinity multiclonal antibodies as primary antibodies. They have been cited more than 25 years ago and are widely used. Here are some examples. Continue reading for more information. Boster Bio: The Best Uses of the OXCT1 Marker
Boster also makes a variety of kits, reagents and primary antibodies. The company offers a broad range of products that include buffers, samples, lysates and controls. Boster Bio is committed maintaining high standards and efficiency during the production process. Boster Bio products are used in many laboratories around the world, and its expertise helps customers reach their research goals.
Boster Bio's antibody are the best choice, whether you need IHC-optimized monoclonal antibodies or WB assays. Boster Bio antibodies have received more 29,000 citations. No matter the application, antibodies from Boster Bio have been validated against a panel that includes more than 250 tissue types. Additionally, they've been tested against untransfected cell lines and recombinant proteins to ensure their high affinity.
These antibodies are popularly used in immunoassays because of their high reactivity as well as specificity. Researchers use primary antibodies in several studies, including Western Blotting, immunohistochemistry, and histopathology. Additionally, scientists use antibodies for measuring specific cell types like CD4+T cells and monocytes. In addition, primary antibodies are also used to detect and measure specific proteins.
Boster's primary antibody products are distinguished by their high specificity and high titers. Their use has been a key factor in many researches over the years. These antibodies can be used in a variety of applications including immunoblotting and ELISA as well as flow cytometry. They are highly suitable for immunocytochemistry and immunofluorescence microscopy, and are highly useful in other applications that require high titers.
An immunoblot is used for determining the specificity or primary antibodies. An immunoblot labels the protein at the correct molecularweight. This procedure can be performed quickly and is very inexpensive. But it reduces the secondary and tertiary structure of the protein, so some antibodies may bind to multiple antigens. This is why Boster’s primary antigens are widely used in ELISA, as well as RIA.
Secondary antibodies can be created by immunizing an animal with primary antibodies from another species. For instance, a goat immunized with purified mouse IgG will produce anti-mouse antibodies that bind to all of the species' IgG fragments. If the goat is immunized only with mouse IgG1 antibodies it will only produce anti-mouse antibody that recognizes the mouse IgG1 proteins.
Boster created the P53 Antibody Assessment (tm) and Picokine (tm). Both products combine innovative technologies to increase the background and enhance sensitivities. Both have been validated on various samples. The validation process and images of the validation are available upon request. The Supervision (tm) ELISA kit is a time-saving tool that saves researchers up to 30 minutes and improves background. Picoband (tm) technology is driven through insights into the best immunogens. It is supported well by technical support.
Primary antibodies of the Boster are basically isolated DNA sequences that have been subject to a variety artificial modifications. While primary antibodies are largely immune-system-specific, secondary antibodies are not. Artificial modifications of antibodies are patent-eligible. Recent U.S. Supreme Court decisions, Mayo v. Myriad, have had a significant effect on patentability of antibody patents. These two decisions have interpreted the scope of an antibody patent.
The written description requirement is a constant source of confusion in antibody patents. This requirement was created by a conflict between USPTO and Federal Circuit. In Noelle v. Lederman, the Federal Circuit ruled that the 'Well-characterized Antigen Test' violated this requirement. This applies to antibodies that bind CD40CR broadly. In light this case, a patent holder cannot broaden his claim to the 'Antibody That Binds CD40CR'.
PMID: 8751852 by Kassovska-Bratinova S., et al. Succinyl CoA:3-oxoacid CoA transferase (SCOT): human cDNA cloning, human chromosomal mapping to 5p13, and mutation detection in a SCOT- deficient patient.
PMID: 10964512 by Fukao T., et al. Succinyl-CoA:3-ketoacid CoA transferase (SCOT): cloning of the human SCOT gene, tertiary structural modeling of the human SCOT monomer, and characterization of three pathogenic mutations.