Otoferlin Antibodies

AND OTOF ELISA kits, Otoferlin Proteins

Many biological assays use antibodies to detect Otoferlin, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Otoferlin in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Otoferlin antibodies...

We validate the specificity of these antibodies to Otoferlin by testing them on tissues known to express OTOF positively and negatively. Browse below to find the OTOF antibody that suites your experiment. We have 1 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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OTOF Infographic by Boster Bio

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Anti-Otoferlin Antibody Picoband®

1 Q&As

Human, Mouse, Rat

IHC, WB

$370
Cat # PB9616
100 μg/vial

OTOF Overview

Facts about Otoferlin.

OTOF 3D structure. Source: alphafold

Otoferlin (OTOF)

Key calcium ion detector involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent way into the presynaptic SNARE proteins in ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter.

Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes (By similarity).

Gene Information

Human
Gene Name:	OTOF
Uniprot:	Q9HC10
Entrez:	9381

Family

Belongs to:

ferlin family

Alternative Names

AUNB1; DFNB6; DFNB9; EC 2.7.10.1; EC 4.2.1.11; FER1L2NSRD9; Fer-1-like protein 2; otoferlin

Molecular Weight

Mass (kDA):
226.753 kDA

Genomic Context


Human

Location:	2p23.3
Sequence:	2; NC_000002.12 (26457203..26558756, complement)

Tissue Specificity

Isoform 1 and isoform 3 are found in adult brain. Isoform 2 is expressed in the fetus and in adult brain, heart, placenta, skeletal muscle and kidney.

Subcellular Localizations

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Cell junction, synapse, presynaptic cell membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Detected at basolateral cell membrane with synaptic vesicles surrounding the ribbon and at the presynaptic plasma membrane in the inner hair cells (IHCs)

Diseases

Complete Hearing Loss
Auditory Neuropathy
Sensorineural Hearing Loss (disorder)
Deaf Mutism
Dystrophy
Deafness Congenital
Hearing Loss, Central
Muscular Dystrophy
Hearing Problem
Vestibulocochlear Nerve Diseases
Distal Muscular Dystrophies
Retinal Degeneration

Malignant Neoplasm Of Breast
Posterior Reversible Encephalopathy Syndrome
Auditory Diseases, Central
Myopathy
Miyoshi Myopathy
Goiter

Pathways

Exocytosis
Membrane Fusion
Localization
Vesicle Fusion
Spermatogenesis
Pathogenesis
Transport
Signal Transmission
Fertilization
Echolocation

Protein Transport
Flight
Cellular Localization
Synaptic Vesicle Exocytosis
Oogenesis

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/OTOF

References

PMID: 10192385 by Yasunaga S., et al. A mutation in OTOF, encoding otoferlin, a FER-1-like protein, causes DFNB9, a nonsyndromic form of deafness.

PMID: 10903124 by Yasunaga S., et al. OTOF encodes multiple long and short isoforms: genetic evidence that the long ones underlie recessive deafness DFNB9.