NME2 Antibodies

Nucleoside diphosphate kinase B (NME2)

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity).

Negatively regulates Rho activity by interacting with AKAP13/LBC (PubMed:15249197). Acts as a transcriptional activator of the MYC gene; binds DNA non- specifically (PubMed:8392752, PubMed:19435876).

Gene Information

Human
Gene Name:	NME2
Uniprot:	P22392
Entrez:	4831

Family

Belongs to:
NDK family

Alternative Names

EC 2.7.13.3; Histidine protein kinase NDKB; MGC2212; NDK B; NDKB; NDP Kinase B2; NDPKB; NDPK-B; NM23B; NM23-H2; NME2; non-metastatic cells 2, protein (NM23B) expressed in; nucleoside diphosphate kinase B; protein (NM23) expressed in

Molecular Weight

Mass (kDA):
17.298 kDA

Genomic Context

Human
Location:	17q21.33
Sequence:	17; NC_000017.11 (51165536..51171744)

Tissue Specificity

Isoform 1 and isoform 3 are ubiquitously expressed.

Subcellular Localizations

Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, ruffle. Isoform 3 is mainly cytoplasmic and isoform 1 and isoform 3 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.

Diseases

• Neoplasms
• Neoplasm Metastasis
• Malignant Neoplasms
• Carcinoma
• Malignant Paraganglionic Neoplasm
• Leukemia
• Myeloid Leukemia
• Melanoma
• Malignant Neoplasm Of Breast
• Myeloid Leukemia, Chronic
• Mammary Neoplasms
• Anemia
• Leukemia, Myelocytic, Acute

• Colorectal Cancer
• Pleural Neoplasms
• Malignant Tumor Of Cervix
• Pleural Mesothelioma
• Ovarian Neoplasm
• Adenocarcinoma
• Neurofibromatosis 1

Interacting Proteins

• DNM2
• NME1
• NME4

Pathways

• Transport
• Localization
• Cell Cycle
• Telomere Binding
• Intracellular Transport
• Cell Activation
• Cell Migration
• Cell Proliferation
• Circadian Rhythm
• Cell Development
• T Cell Activation
• Mitosis
• Dna Repair

• Senescence
• Glucose Homeostasis
• Regeneration
• Flight
• Translation
• Cell Motility
• Cell Communication

PTMs

• Phosphorylation
• Cleavage

• Amidation
• Ubiquitination

Research Areas

• Cell Cycle and Replication
• Core ESC-Like Genes
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NME2

Boster Bio - Best Uses Of The NME2 Marker

We'll be reviewing Steven Boster’s Boster Bio Anti–NME2 marker and the validation on WB. Boster is committed creating the most effective antibody for your research. They also offer valuable information about a range of boster products. Below you will find the answers. We'll also be discussing the Anti-NME2 Marker by Boster Bio and validating the antibodies with known positive and negative samples.

Boster Bio

Boster Bio has been tested as an anti-NME2 immunoglobulin in flow cytometry. It also reacts with Human and Mouse cells. The product has been tested for toxicity in various tests. Boster also thanks the first reviewer for its product. This reward program for scientists is open to everyone.

Steven Boster

Steven Boster is someone you may have heard of if your dreams included being an astronaut or possessing superpowers. But did you know that he was no longer with us? He passed away June 26, 2022. This is his final public appearance. Here are some examples of his greatest uses of NME2 Marker. This GPS device provides accurate, real-time information and is the ideal companion for astronauts.

First, you need be aware the limitations of NME2 Marker. This device is not compatible for use with the GBEEN BEAN. If you are interested in using it for this purpose you should know that it can be used with both GPS and GPSS. Besides that, you should note that the NME2 Marker has several other uses too. One of those uses is to detect satellite locations.

Anti-NME2 Marker

Boster Bio is a company that offers an antibody that detects NME2 in tissue samples. Boster Bio offers high-affinity, primary antibodies that have been developed using research that dates back over 25 years. They are validated by Western Blotting, Immunohistochemistry, and ELISA. Boster offers Anti NME2 anti-nuclear antibodies in a variety o sizes, concentrations, or types.

Validation of antibodies against WB

Validation and validation of antibodies on WB using cytoplasmic markers NME2 is crucial for reliable detection. Human NME1 bonds to the protein dynamin. High levels of GTP are required to promote membrane formation and endocytosis. High GTP levels are essential for the clearance of cell surface receptors. However, the NME2 mAb may have cross-reactivity in certain tissues.

To validate an antibody, knockout mice were used. This technique allows for the elimination or reduction of a target protein. The antibody can detect mutations and truncations of the protein. Therefore, gene editing could affect the assay results. Gene editing may alter the protein's structure in a way that prevents the expression of the target protein. However, positive results from gene knockout mice can indicate that the primary anti-body is not highly specific. Transient gene KD could be used in place of knockout validation. Although knockout validation takes longer and is more expensive, it is still a useful tool to validate antibodies.

The specificity of the antibody is a key factor in validation of antibodies on WB using NME2-marker. This is essential for pan/PTM tests because both modified and unmodified target forms will comigrate closely. This requires separate Westernblottings and testing using mismatched secondary antibodies. This avoids the appearance of cross-reactive bands on the WB, which could have a detrimental effect on the data interpretation and analysis.

As a rule, antibodies showing positive WB results should also show positive IHC, ICC or IF results. Recent data from immunoblotting in human patients suggests that WB is the first step to validate antibodies. However, the method is not perfect. However, flawed data can result from many studies that used antibodies that had not been validated. To ensure optimal performance, it is important that antibodies with knockout mice are evaluated.

The use of assay-specific validation of antibodies is crucial for reproducibility of research findings. Because of inconsistent antibody performance and a lack of accepted standards in immunoassay environments, it is essential to evaluate antibodies to ensure they perform as intended. WB validation aims to confirm the primary antibodies' specificity and selectivity. It can also be performed with complementary methods to confirm the specificity of the antibody.