Beta-nerve growth factor Antibodies

Beta-nerve growth factor (NGF)

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.

Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). .

Gene Information

Human
Gene Name:	NGF
Uniprot:	P01138
Entrez:	4803

Family

Belongs to:
NGF-beta family

Alternative Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

Molecular Weight

Mass (kDA):
26.959 kDA

Genomic Context

Human
Location:	1p13.2
Sequence:	1; NC_000001.11 (115285915..115338253, complement)

Subcellular Localizations

Secreted. Endosome lumen. ProNGF is endocytosed after binding to the cell surface receptor formed by SORT1 and NGFR.

Diseases

• Nervousness
• Pheochromocytoma
• Alzheimer's Disease
• Inflammation
• Pain
• Neoplasms
• Neurodegenerative Disorders
• Nerve Degeneration
• Adrenal Gland Neoplasms
• Neuroblastoma
• Hyperalgesia
• Malignant Neoplasms

• Nerve Damage
• Tissue Adhesions
• Ischemia
• Neurotoxicity Syndromes
• Brain Injuries
• Diabetes Mellitus
• Atrophy

Interacting Proteins

• NGFR
• Ngfr
• NTRK1
• SORT1

Pathways

• Cell Death
• Regeneration
• Secretion
• Innervation
• Transport
• Localization
• Cell Proliferation
• Pathogenesis
• Cell Differentiation
• Cell Growth
• Aging
• Neuroprotection
• Cell Cycle

• Programmed Cell Death
• Cell Adhesion
• Sensitization
• Neurogenesis
• Angiogenesis
• Translation
• Hypersensitivity

PTMs

• Phosphorylation
• Cleavage
• Dephosphorylation
• Glycosylation
• Methylation
• Reduction
• Acetylation
• Iodination
• Ubiquitination
• Biotinylation

• Oxidation
• Nitration
• Phosphorothioation
• Myristoylation
• Prenylation
• Ribosylation
• Deacetylation
• Demethylation
• Nitrosylation
• Farnesylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NGF

Boster Bio - Best Uses Of The NGF Marker

Nerve growth factor (NGF) is a polypeptide that regulates development and differentiation of the sensory and sympathetic neurons. It is used in a variety of areas that include the discovery of drugs as well as cancer research, and medical diagnosis. This Boster Bio review will provide additional information about the NGF marker. This article will provide information on how this marker can be used to improve treatment outcomes for various conditions.

The growth factor for nerves is a polypeptide

Nerve growth factor (NGF), a polypeptide regulates the differentiation, proliferation and the survival of neurons. The sequence of its nucleotides is similar to beta-NGF's sequence in mice. It is located on chromosome 3p22 of humans, and a small part of the human chromosome lies on the proximal end of the short arm. The NGF encodes the human nerve growth gene. The extracellular domain of the polypeptide is responsible for its function. The mutations in this gene can cause hereditary sensory neuropathy and loss of perception of pain.

NGF is a major neurotransmitter that regulates phenotypic features in the noradrenergic nuclei of the CNS. It participates in central regulation of autonomic function and regulates the activity of stress axis. NGF is neurotransmitter which helps neurons respond to stimuli when present in sufficient quantities. It also aids in the development of new nerve cells, which are necessary for maintaining the nervous system.

NGF is an trophic protein that regulates the development of targeted neurons in the peripheral and central nervous systems. The sequences of its molecule reveal the extensive homology and diversity between species. NGF is also involved in a variety of biological activities. It is responsible for the survival and proliferation of beta cells in the pancreas. It also plays a significant role in controlling the immune system. This means that nerve growth factor is a peptide in Boster Bio that aids people who suffer from neurological issues.

It is a neurotrophic factor

It is an anabolic protein that is naturally produced by the human body. It is involved in learning and memory and influences the growth of nerves. It is responsible for the regulation of mitochondria as well as the growth of neuron cells. In addition, it serves as a protective factor for cartilage and bones. It's also been demonstrated to regulate insulin receptors. Consider taking a high dose supplement that contains this substance if you have diabetes.

The neurotrophic elements play an important role in the development and maintenance of the nervous system, they are also promising drug candidates for a variety of neurodegenerative diseases. This article reviews current efforts to overcome issues that neurotrophins are facing as they transition from lab-based research to the clinic. The article also provides a roadmap for future studies that are translational. Neurotrophins, for instance, are being investigated in clinical trials to treat Huntington's disease, Parkinson's disease, and obesity.

The function of neurotrophic factors is dependent on the concentrations of these neurotransmitters within the nervous system, as well as the types of receptors found in the cells. In the peripheral nervous system, NGF dominates, acting on sensory and sympathetic neurons. BDNF dominates the central nervous system due to its high TrkB. BDNF plays a crucial biological role in inflammation and is crucial for the normal functioning of the nervous system.

It regulates the growth and differentiation of certain sympathetic and sensory neurons.

The NGF gene is involved in the survival and growth of nerve cells. It is involved in the growth and maintenance of sympathetic and sensory neurons. NGF can activate genes by regulating their expression via the Tropomyosin receptor kinase. The PI 3-kinase and PLC signaling pathways are activated when NGF is present. This could be due to the relationship between NGF as well as the survival and growth of sympathetic and sensory neurons.

GFL development in peripheral nervous system is controlled by the NGF gene. Researchers believe that this gene is involved in neuronal differentiation. This gene is located in the sympathetic ganglia as well in the dorsal root ganglia. Although it does not alter GFRalpha3 or GFRalpha1 the expression of this gene is decreased by 25% and 60 percent in ret mutants at P2 and at P10, respectively.

Different ways can regulate the expression of NGF marker in DRG neuron cells. In wild-type mice, 68% of the C fibre soma is IB4-positive while in the GFRalpha2-overexpressing mice, all 20 cells were IB4-positive. IB4-positive cells also have a decreased central projection pattern, as well as sensitivity to TGRP.

It's a dimmer

Boster Bio NGF markers are dimers of human 2.5S NGF. They are a polypeptide chain consisting of 120 amino acids that are linked together. This antibody reacts with Human and Rat tissues. The product can be stored at -20°C for one year. It is available as an reagent in a drop-on demand format. The Boster Bio NGF marker is verified on positive and negative samples.

It is replaced by serum-free medium RPMI 1640

The RPMI 1640 Medium contains a reducing agent called Glutathione and a large amount of vitamins that include biotin. The medium is made up of 10 Fetal Bovine serum and requires 5-10% CO2 to grow. The medium is compatible with a variety of mammalian cell types, including heLa as well as Jurkat cells. These modifications are added to the RPMI medium for Boster Bio NGF assay.

The cells were placed on glass coverslips to be cultured for 24 hrs. After 24 hours, the cells were rinsed in PBS three times before being fixed in 4% paraformaldehyde. The cells were then incubated using primary antibodies against human fibrill growth factor (GFP) or human NGF (1/100). Then, cells were incubated for a night with FITC-conjugated secondary antibody. Nuclei were visualized with 4,6-diamidino-2-phenylindole staining. Images were taken using a Zeiss LSM710 confocular microscope.

The cells were tested in the same manner as the mice to determine if they were able to contain Boster Bio NGF. The lower wells were filled with 500ml of migration medium containing gentamicin 0.3% BSA, and 100ng/ml SDF-1a. Then, the cells were seeded on transwell inserts with 24 wells and allowed to migrate through the chamber for 4 h in an incubator that was CO2 at 37°C.

It is a primary antigen

Nerve growth factor, also referred to as NGF is a protein which regulates the growth and differentiation of sensory and sympathetic nerve cells. The beta-subunit in nerve growing factor has been assigned to distinct chromosomes by humans and mice. The mutations in the gene can cause loss of perception of pain. Boster Bio NGF Marker is an antibody that is primary and whose affinity for the receptor is higher than 97%.

Using the NGF Marker, researchers are able to analyze the differentiation and expression of NGF-NSCs in DPSCs. Neurospheres were fixed by using PFA 4%, permeated using 0.1 percent Triton, and blotted using an H2O2-blocking solution. The neurospheres were stained with diaminobenzidine trihydrochloride along with secondary and primary antibodies.

Scientists can ask more questions about specimens using primary and secondary antibodies by studying the behavior of different types of cells. In addition, they can gather more information from the context and stronger answers. Because the primary antibody can recognize molecules, and secondary antibodies can recognize secondary peptides This is possible. The primary antibodies are the primary components of immunoassays. The antibodies are used in Western Blotting, immunohistochemistry, histopathology tests, and ELISA, which is short for Enzyme-Linked Immunosorbent Assay.

Boster Bio has it

Boster Bio is the best place to locate high-quality antibodies, whether you are an expert in immunohistochemistry or researcher searching for the latest antibody. Boster Bio's antibody inventory includes more than 16,000 antibodies that are valid for ELISA/WB, FC, IHC and FC. Boster also offers rabbit polyclonal antibodies. When you purchase a primary antibody from Boster Bio also includes a secondary antibody that is free. The company manufactures antibodies in its own facilities in order to maintain the highest standards of quality and efficiency.

Founded in 1993, Boster Biological Technology, Ltd. offers ELISA kits including antibodies, as well as related products. The company also offers custom mouse monoclonal antibodies, recombinant proteins expression assays, multiplex cytokine assays, and other biomarker products. The company is based in Japan and offers a variety of services. These services enable researchers to quickly detect and analyze biomarkers found in various samples.

High-quality ELISA kits are crucial to successful immunoassays. Boster Bio's ELISA kit has been validated for biologically relevant matrices. All Boster ELISA kits come with validation procedures and validation images. Boster also backs its products with technical assistance to assist scientists in finding the most effective antibodies for their research. The company's products are utilized by researchers all over the world, and over 29,000 publications have been cited.