5'-nucleotidase Antibodies

5'-nucleotidase (NT5E)

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase actions.

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Gene Information

Human
Gene Name:	NT5E
Uniprot:	P21589
Entrez:	4907

Family

Belongs to:
5'-nucleotidase family

Alternative Names

5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase

Molecular Weight

Mass (kDA):
63.368 kDA

Genomic Context

Human
Location:	6q14.3
Sequence:	6; NC_000006.12 (85449584..85495791)

Subcellular Localizations

Cell membrane; Lipid-anchor, GPI-anchor.

Diseases

• Malignant Neoplasms
• Calcinosis
• Atherosclerosis
• Neoplasms
• Tissue Adhesions
• Pain
• Inflammation
• Neoplasm Metastasis
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Malignant Paraganglionic Neoplasm
• Brain Neoplasms
• Leukemia

• Pseudoxanthoma Elasticum
• Inflammatory Bowel Diseases
• Glioma
• Intestinal Diseases
• Carcinoma
• Celiac Disease
• Neuralgia

Pathways

• Transport
• Cell Proliferation
• Localization
• Methylation
• Cell Adhesion
• Cell Activation
• Immune Response
• Chemotaxis
• Regeneration
• Cell Death
• Pathogenesis
• Secretion
• Cellular Homeostasis

• T Cell Activation
• Neutrophil Extravasation
• Virulence
• Response To Paclitaxel
• Inflammatory Response
• Platelet Aggregation
• Chemokine Secretion

PTMs

• Methylation
• Demethylation

• Dephosphorylation
• Gpi Anchoring

• Myristoylation

Research Areas

• Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NT5E

Best Uses Of The NT5E Marker

Researchers who are interested in studying NT5E will discover high-connectivity DEPs as well as Boster Bio Anti CD73/Nt5E Picoband(tm) extremely useful products. The antibody is tested for mouse, human, and rat reactions in IF and IHC. It reacts with NT3A, NT3B and other markers.

Boster Bio

The NT5E gene encodes an plasma membrane protein that facilitates the conversion of extracellular nucleotides to membrane-permeable nucleoside. It is a major factor in the development of lymphocytes. Defects in this gene may lead to calcification of the joints and arteries. There are two variants of the transcript for this gene. Boster's NT5E marker is able to validate all antibodies to verify the accuracy of the test.

Boster Bio Anti-CD73/NT5E Antibody is part of the Picoband(tm) catalog. This antibody reacts with Mouse, Rat, and Human cells. It is a source of Trehalose. It can be utilized in ELISA or flow Cytometry. The Best Uses Of The Marker NT5E

Boster Bio Anti-CD73/Nt5e Antibody Picoband(tm)

The Boster Bio Anti-CD73/N5e Fab-ZAP Saporin conjugate has been tested to be used in immunohistochemistry tests. It is able to measure the internalization of antibodies, and it is able to be compared to the isotype control antibody (MEDI9447). The CellTiterGlo assay was used to determine the effects of the FabZAP 50 uL reaction added to cell lines.

The Boster Bio Anti-CD73/N5E Antibody is part of the Picoband(tm) catalog and has been tested using ELISA and Flow Cytometry. It reacts with Rat, Mouse, and NT5E. Trehalose and other additives are also included in the reagent to ensure security and stability.

Boster Bio Anti CD73/N5e Antibody Picoband(t) is found to inhibit CD73 through increased leukocyte clustering. The inhibitor blocks CD73 while increasing levels of TH2 cytokines. You can test the product's reactivity using the purified CD4+ lymphocytes with an AMP suppression test.

The CD73/Nt5e antibody targets the receptor for adenosine to block the function of the tumor's microenvironment. This type of antibody has an additional effect on T-cells by stimulation of lymphocytes that are specifically targeted to tumors. In murine xenografts anti-mouse rat antibody TY23 was extensively employed. It was previously reported in MedImmune.

NT5E Marker

NT5E is a gene that is highly expressed in a variety of cancers. HNSCC is one of them. GEPIA 2.0 and Oncomine showed the same results. HNSCC has statistically significant NT5E expression. However there is no evidence that NT5E expression is associated with increased survival rates for patients. It is too uncertain whether NT5E expression affects the prognosis of HNSCC.

Previous studies have suggested that NT5E may be involved in the development of breast cancer, and is crucial in the formation of metastases to breast cancer. Despite this association, very few studies that have focused on translation have been done. Kruger et al. found that the gene suppresses ERa-mediated tumor growth. This discovery has important implications for future research. A gene signature can be used to determine metastatic potential. This will increase the chances of detecting cancer before it grows.

The gene NT5E encodes for the protein CD73. It is located on the long arm of chromosome 6 between positions 14 and 21. NT5E has two isoforms which are variant one and two. The first encodes a longer isoform than the second and variant two does not have the alternative exon in-frame. NT5E has the transcript length of 3384 bp , and a translation length of 524 residues. It is classified as a hypoxia-inducible target gene.

The methylation of NT5E CpG islands is a promising biomarker to detect breast cancer. The methylation-dependent silencing of NT5E is also associated with acquired resistance to chemotherapy. It is also found in tumors that have hormone receptor negative symptoms. The gene is regulated by a complicated pathway that involves NT5E methylation.

DEPs with high-connectivity

Bostern Bio's High-connectivity DEPs are designed to boost your research. These high-connectivity DEPs permit you to analyze and manipulate DNA in order to increase the accuracy of your study. Boster Bio optimization guides provide valuable information and tips to optimize your experiments. They cover a variety of aspects of ELISA. They also provide details on the specific diseases that require it as well as tips for optimizing your experiments as well as recommended products.