NRG2 Antibodies

Pro-neuregulin-2, membrane-bound isoform (NRG2)

Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors.

May also promote the heterodimerization with the EGF receptor. .

Gene Information

Human
Gene Name:	NRG2
Uniprot:	O14511
Entrez:	9542

Family

Belongs to:
neuregulin family

Alternative Names

divergent of neuregulin-1; DON1; Don-1; HRG2; neuregulin 2; NTAKneural- and thymus-derived activator for ErbB kinases; pro-neuregulin-2, membrane-bound isoform; pro-NRG2

Molecular Weight

Mass (kDA):
91.679 kDA

Genomic Context

Human
Location:	5q31.2
Sequence:	5; NC_000005.10 (139846779..140043675, complement)

Tissue Specificity

Restricted to the cerebellum in the adult.

Subcellular Localizations

[Pro-neuregulin-2, membrane-bound isoform]: Cell membrane; Single-pass type I membrane protein. Does not seem to be active.; [Neuregulin-2]: Secreted.

Diseases

• Malignant Neoplasms
• Neoplasms
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Nervousness
• Schizophrenia
• Carcinoma
• Congenital Abnormality
• Muscle Hypotonia
• Heart Diseases
• Carcinogenesis
• Congenital Heart Defects

• Neuroblastoma
• Developmental Delay (disorder)
• Tissue Adhesions
• Myasthenias
• Neoplasm Metastasis
• Spinal Cord Injuries
• Glaucoma, Open-angle

Pathways

• Cell Growth
• Pathogenesis
• Invasive Growth
• Brain Development
• Myelination
• Cell Proliferation
• Biofilm Formation
• Cell Death
• Transport
• Cell Adhesion
• Cell Migration
• Mammary Gland Morphogenesis
• Neurogenesis

• Heart Formation
• Cell Cycle
• Cell Differentiation
• Glial Cell Growth
• Mammary Placode Formation
• Nitrate Transport
• Gland Morphogenesis

PTMs

• Phosphorylation
• Cleavage

• Nitration
• Glycosylation

• Ubiquitination

Research Areas

• Apoptosis
• Cancer

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NRG2

Boster Bio - Best Uses Of The NRG2 Marker

Boster Bio - What is Steven Boster's contribution to this field? This article will cover Steven Boster’s work, his primary antibody and the best use of the NRG2 mark. Boster is a world-renowned scientist, and I hope you'll take the time read this article. It is hoped that you will find it both entertaining and informative.

Steven Boster

Steve Boster was born in Joliet, Illinois. He died June 6, 2022. Born in Joliet, IL, he was an active member of Concordia Hall in Staunton. He was the father Donald, Sr., David Boster, Nina Mae Hall. Frances of Verona (WI) and his daughters Kimberly, Tammy, and Tammy of Herrin (IL) are his survivors. He is also survived by his four brothers, as well as several nieces and nephews.

His primary antibodies

NRG2 is made by a variety of cell processes, including the cell-cycle. It is synthesized by the cell cycle as a membrane precursor. It is then converted to signalling-competent ErbB receptor ligands via ubiquitin dependent alpha and beta secretases. NRG2 is a suitable substrate for alpha-secretases and is found at high levels in PC12 cells, which accumulate only little pro-NRG2 protein. This makes NRG2 a good substrate for alpha secretases. It may also be used to unmask constitutive shredding activity.

The His tag is a sequence of six to nine hetidine residues fused together to form a protein. It is less likely to obstruct the structure of the target protein and is more compatible with use under denaturing conditions. His-tags can bind to immobilized metalions. Antibodies to His tagged proteins provide a reliable method of detecting tagged target protein.

The NRG2 proteins were also tested using custom and commercial antibodies. This antibody recognizes NRG2 endogenous levels. The antibody's target specificity is also determined by its specificity for the NRG2 marker. It can recognize both circulating and non-recombinant NRG2 proteins. It is highly recommended you buy an antibody only from a reputable supplier in order to ensure the highest quality.

Double-label fluorescence ISH displays overlapping signals in mouse hippocampus. The hippocampus contains a pyramidal cell layer. The NRG2 signal is decreased in ErbB4-negative cell. The ECD (ECD), TM (and ICD) were identified as NRG2 immunogen sites. These images show NRG2 surface puncta from immunooreactive cells in the CA1 and CA1 stratum pyramidale.

NRG2-ECD is a successful immunoprecipitation technique for ErbB4-interacting protein. It is an immunoprecipitation technique that combines an antibody with an antibody that recognizes ErbB4-interacting protein. The antibodies are then used to select protein bands for mass spectrometry analysis. The corresponding protein band was then purified and immunoprecipitated via ErbB4mAB.

After the incubation with primary antibodies, the cells were stained with fluorescent secondary antibodies. These fluorescent antibodies were incubated for 45 minutes at RT with the tissues. After washing the cells, they were mounted onto gelatin-coated slide. The images of fluorescent cells were taken using a Zeiss LSM510 Meta Confocal microscope. They were then analysed in Adobe Photoshop. If desired, we can repeat this analysis with different antibodies.

His research

The role of NRG2 markers in migration is controversial. Although there are many theories, we don't know how it works. One possibility is that the marker regulates the amount of Dlx2 expressed on cells. This suggests that a functional NRG2 shaddase is required for migration. It may also play a role in the regulation of activity of other cell surface receptors like integrins.

In addition, this marker is involved in neuronal proliferation and migration. It is involved in a process called mitosis. NRG promotes in vivo the formation of new neurons within the SVZ or RMS. This effect is not seen in cells that lack the NRG2 proteins. NRG2 is shown to increase the number of cells that are positive in the lab for other neuronal markers such as GABA.