MID1 Antibodies

AND MID1 ELISA kits, MID1 Proteins

Many biological assays use antibodies to detect MID1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with MID1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop MID1 antibodies...

We validate the specificity of these antibodies to MID1 by testing them on tissues known to express MID1 positively and negatively. Browse below to find the MID1 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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MID1 Infographic by Boster Bio

All MID1 Products

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Product

Figures

Specificity

Applications

Price

Anti-TRI18 MID1 Antibody

4 Q&As

Human, Mouse, Rat

IF, IHC, WB

$360
Cat # A01774
100 µl

Anti-MID1 Antibody Picoband®

1 Q&As

Human, Mouse

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A01774-1
100 μg/vial

TRI18 Colorimetric Cell-Based ELISA

Human, Mouse, Rat

ELISA

$480
Cat # EKC1628
1 kit, containing one 96-well plate and all necessary reagents

MID1 (NM_000381) Human Recombinant Protein

$1249
Cat # PROTO15344
20 µg

MID1 Overview

Facts about E3 ubiquitin-protein ligase Midline-1.

MID1 3D structure. Source: alphafold

E3 ubiquitin-protein ligase Midline-1 (MID1)

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which ends in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. .

Gene Information

Human
Gene Name:	MID1
Uniprot:	O15344
Entrez:	4281

Family

Belongs to:

TRIM/RBCC family

Alternative Names

EC 6.3.2.-; FLJ57031; FLJ58683; FLJ76288; FXYGBBB1; Midin; midline 1 (Opitz/BBB syndrome); RNF59MIDIN; TRIM18mouse, homolog of

Molecular Weight

Mass (kDA):
75.251 kDA

Genomic Context


Human

Location:	Xp22.2
Sequence:	X; NC_000023.11 (10445310..10833683, complement)

Tissue Specificity

In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.

Subcellular Localizations

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.