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- Table of Contents
Facts about Mediator of RNA polymerase II transcription subunit 4.
Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. .
Human | |
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Gene Name: | MED4 |
Uniprot: | Q9NPJ6 |
Entrez: | 29079 |
Belongs to: |
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Mediator complex subunit 4 family |
Activator-recruited cofactor 36 kDa component; ARC36; DRIP36; DRIP36mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae); FLJ10956; HSPC126; HSPC126vitamin D receptor interacting protein; MED4; mediator complex subunit 4TRAP/SMCC/PC2 subunit p36 subunit; subunit 4 homolog; TRAP36; VDRIP
Mass (kDA):
29.745 kDA
Human | |
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Location: | 13q14.2 |
Sequence: | 13; NC_000013.11 (48075724..48095141, complement) |
Nucleus.
If you are looking for high affinity primary antibodies for MED4 Marker, then you have come to right place. These antibodies are widely cited in the scientific literature and have been validated on a variety of different assays, including Western Blotting, Immunohistochemistry, and ELISA. This boster Bio antibody is specifically made for this specific molecule. It has an excellent affinity for binding its target protein.
After a lengthy battle with COVID-19, Steve Boster was a respected physician and Emergency Medicine practitioner. He passed away in Madison, WI. Born and raised in Joliet, IL, Boster practiced Emergency Medicine for 33 years before taking up painting full time in 2015. He has been a hunting and fishing enthusiast his entire life and enjoyed using vivid colors. Steve Boster donated a portion the sale price each painting to The Fisher House Foundation, which provides free meals for veterans in hospital.
MED4 is a novel genetic code that encodes a protein which is expressed on cells. This protein is expressed by many types of cells. Boster Bio has a highly specific MED4 anti-body that can be used to detect MED4 protein. BosterBio offers a range of MED4 antibodies. Boster Bio has both monoclonal and multiclonal antibodies to the MED4 Protein.
It is not easy to create primary antibodies with high affinity. This can depend on the antigen and require elution using harsher methods. High-affinity antibodies are often used for the detection of a specific epitope. They can also elicit neutralizing responses by binding epitopes. Strong neutralizing response can be elicited from antibodies targeting spike protein and nucleocapsid proteoprotein.
The degree to which an antibody binds to one specific ligand is called its affinity. The equilibrium dissociation constant (KD) is a common measure for this. The KD value is proportional to the concentration of the reactants and equals their rate of dissociation into their constituent parts. You can measure the rate constants for the reaction to calculate the KD value. The higher the affinity of an antibody, the smaller the KD value.
A phage ELISA, which is a method to detect the antigen in addition to the MED4 mark, is also available. Primary antibodies can be raised against any antigen using this method. They are capable of recognizing post-translational modification and analysing living cell components at a molecular level. Furthermore, high-affinity primary antibodies are validated in multiple research applications. The antibodies are tested using multiple species, and an increasing number of them have undergone knockout validation or protein array validation.
Another method is to make an immunoassay from this IFNg-g scFv. The scFv were purified by Ni2+ affinity Chromatography. The results showed that the scFv products had high activity against the IFN-g antigen in real samples. The method is simple and doesn't require complicated instruments. This test is also fast and simple.
Bio-panning was done to screen for binding activities after the MBP-Lk-ScFv clone was obtained. After imaging and photofixing a clear band was seen at 20 kDa. This indicates that MBP–Lk–scFv antibody bound with the IFN–g. An immunoassay was not performed on a negative control.
Boster Bio MMP13 antibody is highly specific for MMP13 proteins and uses the MED4 marker. These antibodies are made from monoclonal antibodies developed in-house and derived from the most widely used clones. The company also offers multiple conjugates that can be used for different applications. Customers can choose the appropriate antibodies for their specific application. As a result, the Boster Bio MMP13 antibody can help researchers in the field determine the role of the enzyme in the disease process.
PMID: 10235266 by Rachez C., et al. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.
PMID: 10882111 by Malik S., et al. The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs.