MDH2 Antibodies

Malate dehydrogenase, mitochondrial (MDH2)

Gene Information

Human
Gene Name:	MDH2
Uniprot:	P40926
Entrez:	4191

Family

Belongs to:
LDH/MDH superfamily

Alternative Names

EC 1.1.1; EC 1.1.1.37; malate dehydrogenase 2, NAD (mitochondrial); MDH; MGC:3559; mitochondrial; MOR1

Molecular Weight

Mass (kDA):
35.503 kDA

Genomic Context

Human
Location:	7q11.23
Sequence:	7; NC_000007.14 (76048106..76067508)

Subcellular Localizations

Mitochondrion matrix.

Diseases

• Malnutrition
• Melanoma
• Malignant Neoplasms
• Neoplasms
• Fish Diseases
• Obesity
• Prediabetes Syndrome
• Liver Diseases
• Classical Galactosemia
• Neoplasms, Radiation-induced
• Drug-induced Liver Injury
• Hypoxia

• Neoplasm Invasiveness
• Carcinoma
• Ulcer
• Insulin Resistance
• Alcoholic Liver Diseases
• Patient Dependence On
• Mammary Neoplasms

Pathways

• Localization
• Gluconeogenesis
• Glyoxylate Cycle
• Proteolysis
• Fermentation
• Translation
• Transport
• Chromosome Localization
• Programmed Cell Death
• Methanol Oxidation
• Tricarboxylic Acid Cycle
• Pigmentation
• Energy Homeostasis

• Malate-aspartate Shuttle
• Urea Cycle
• Cellular Localization
• Cell Death
• Pathogenesis
• Fatty Acid Oxidation
• Glycolysis

PTMs

• Phosphorylation
• Oxidation

• Reduction
• Cleavage

• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MDH2

Boster Bio Antibodies - Best Uses Of The MDH2 Marker

If you're looking for an MDH2 antibody, you should read this article. It will show you how Boster validates its antibodies on immunofluorescence, WB, and ICC platforms. Read on to discover which Boster antibodies are the most effective and which ones may not work for you. We'll also explain why Boster is the best choice when it comes to mdh2 antibodies.

Boster validates all antibodies on WB, IHC

Boster Bio, a leader in producing high-specificity, highly sensitive antibodies, is an excellent resource for your research. The company's lab delivers antibodies, reagents, and ELISA kits that are rigorously tested and validated. Using the Boster Quality Guarantee, you can rest assured that each product will work as it is supposed to. Boster also offers a comprehensive range of antibodies for WB, IHC, and ELISA.

For the highest-quality reagents, select antibodies from Boster Bio. The company offers more than 16,000 antibodies for WB, IHC, ELISA, and FC. The company's antibody collection includes rabbit polyclonal antibodies. Purchasing an antibody from Boster allows you to receive a free secondary antibody with the purchase of a primary one. Moreover, you can use the Boster Bio antibodies for mouse and human samples. All of its antibodies are validated for use in Western blotting and immunohistochemistry (WFA), and the company's QC and quality assurance programs ensure that Boster produces high-quality products that are reliable and dependable.

While WB is not a perfect validation method, it is an effective tool for antibody screening. WB and IHC provide a high correlation and can be used to determine the specificity of antibodies. However, knockout animals are also imperfect and should be used as a last resort. So, it is best to validate antibodies on WB, IHC, and IF before performing experiments with them.

WB experiments are time-consuming. Samples are loaded onto an SDS-PAGE gel and electrophoresed for 1.5 hours. After this, the proteins are transferred to a membrane. The transfer process can take another hour or more. The membrane is then blocked for an hour and incubated with the primary antibody for two to twelve hours. In this way, the entire experiment can take 6.5 to 16.5 hours.

Boster validates all antibodies on ICC

Boster Bio provides high-affinity primary antibodies for immunohistochemistry (ICC). These antibodies have been validated for ICC and Western Blotting and have high citations from the scientific community. These antibodies are also flow validated and should be stored undiluted at 4degC. They have high affinity and sensitivity and are ideal for routine ICC applications. Boster also offers FITC conjugated monoclonal antibodies for ICC.

Boster validates all antibodies on Immunofluorescence

Before purchasing an antibody, be sure to read the product datasheet to learn how to use it. During the validation process, Boster scientists test each antibody to ensure optimal performance and sensitivity. In addition to the information in the datasheet, the protocol page also contains information about the most common methods for using the antibody. After ensuring that the product is suitable for your application, you may want to try it out yourself by following one or several protocols.

One way to validate an antibody is to perform a multiplex immunofluorescence experiment. Multiplex immunofluorescence requires three or more antibodies. A single antibody may only detect one target. This can cause multiplex staining. This method allows researchers to compare a variety of antibodies at the same time to determine which ones give the most consistent results. The results of this study indicate that multiplex immunofluorescence is the most accurate method for identifying and detecting cancer cells.

The method employed to validate all antibodies on Immunofluorescence is based on the Tukey test. A fourfold increase in titers in two consecutive patient sera is necessary to conclude that an antibody is positive. However, a single IgM positive test may be presumptive, and should be confirmed using a virus isolation, RT-PCR, or PRNT. A PRNT will distinguish between potential cross-reactive antibodies from genuine ones.