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3 Q&As
Facts about DNA replication licensing factor MCM5.
The six ATPase active sites, however, are likely to contribute differentially to the intricate helicase activity (By similarity). Interacts with MCMBP.
Human | |
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Gene Name: | MCM5 |
Uniprot: | P33992 |
Entrez: | 4174 |
Belongs to: |
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MCM family |
CDC46 homolog; CDC46DNA replication licensing factor MCM5; EC 3.6.4.12; MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S.cerevisiae); MCM5 minichromosome maintenance deficient 5, cell division cycle 46; MGC5315; minichromosome maintenance complex component 5; minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46); minichromosome maintenance deficient 5 (cell division cycle 46); P1-CDC46
Mass (kDA):
82.286 kDA
Human | |
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Location: | 22q12.3 |
Sequence: | 22; NC_000022.11 (35400133..35454942) |
Nucleus. Cytoplasm, cytosol.
Antibodies against the MCM5 marker can be purchased at various places, including Boster Bio. This article will review their monoclonal anti-desmin antibodies, Boster Bio Anti-MCM5 cell-based ELISA Kit and Boster Bio Anti-Glycophorin / CD235a Monoclonal Antibody. Boster Bio products are validated across multiple platforms, including known positive and negative samples. In addition to the high affinity and specificity of their products, Boster will give product credits to first reviewers and all scientists from around the world.
Boster Bio's anti-MCM5 monoclonal anti MCM5 antibody has been extensively validated. It was created from a mouse clone that reacts to MCM5 markers. This antibody is suitable for conjugation to a wide range of different reagents. The company tests its antibodies against known positive and negative samples. The antibody is available in liquid form.
The monoclonal antibody was created from studies that looked at 66 intracranial meningiomas. The sample group included benign, rare, and recurrent tumors. Eight brain tumors invasive to the brain were also identified by the nuclear antigen Ki67. Through this marker, the antibody can detect the cancer-causing cell.
Boster Bio Anti-MCM5 Cell Based ELISA Kit provides a highly sensitive, flexible antibody for research applications. It has been tested for IHC-P, ICC, and WB. It is compatible with mouse, human and the rat tissues. It also contains trehalose, which makes it easier to utilize an elisa kits. A buffer that is made up of 0.1 percent tween-20 also included in the kit.
The MCM5 Cell-Based ELISA Kit was developed to detect MCM5 and to determine the effects of various stimulation conditions on the expression of MCM5 in different cell forms. It is comprised of primary antibodies against MCM5 and secondary HRP-conjugated antisense antibodies against this protein. The secondary antibody is bonded to an enzyme (HRP) that catalyzes the colorimetric reaction. The Boster Bio Anti-MCM5 Cell-Based ELISA Kit includes multiple methods of normalization for data acquisition.
This antibody reacts to glycophorin A/CD235a, which is found on the surface eukaryotic cell cells. This monoclonal antibody was created against seven human-recombinant proteins, including desmoglein 2 and a domain called coiled coil protein. It also targets the platelet F11 receptor. It also stains cells in live hPSCs, however its performance in fixed cells is less consistent.
This monoclonal antibody can be used to detect Glycophorin A (CD235a) and CD235a (a cell-surface protein which bonds with the MCM5 marker). It recognizes CD235a and Glycophorin A on human cells. The MCM5 marker is located on the cell's surface throughout the body, including breast tissue.
Boster Bio's product range comprises a variety of reagents aimed at diverse biological functions. Boster Bio offers products for hormones, cardiac markers, toxins, as well as human proteins. Additionally, the company has developed and commercialized several high-quality immunoassays. Its products include diagnostic-grade antibodies solutions tests for food safety and autoimmune disease treatments.
The MCM5 marker was used as the binding determinant. The mAbs separated from the supernatants using affinity chromatography using GE Lifesciences mAb Select Sure recombinant proteins A and G sepharose HP columns. After the purification process, they were concentrated by centrifuge, and then diluted in CMF-PBS containing 0.02% v/v azide.
Boster Bio Anti-GlycophorIN A / CD235a Monoclonally-Analysis Using the MCM5 Marker
This mAb was employed in studies in which hiPS cells were transformed. It was used in experiments to test the comparison of hiPSCs against human embryonic stem cells. The mAb immunoreactivity of the mAb was extremely high and was seen in many hPSCs. In a study of phase-imaging, the sensitivity of the mAb was also measured.
Boster Bio Anti–GlycophorINA / CD235a MonoclonallyAntibody is highly sensitive. It also utilizes the MCM5 marker for HPSCs with no differentiation. It has been proven to be a valuable marker for pluripotency and is effective in a variety.
This mAb is highly sensitive specific MCM5 antibody that is able to bind DSG2 glycoproteins. It is extremely specific, allowing it to target cancerous cells. The DSG2 marker is expressed on a functional subset bone marrow fibroblasts. It can regulate the expression of hematopoietic cytokines.
PMID: 8265339 by Hu B., et al. The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins.
PMID: 16899510 by Tsuji T., et al. Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells.