MAPK12 Antibodies

Mitogen-activated protein kinase 12 (MAPK12)

Serine/threonine kinase which functions as an essential part of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress resulting in direct activation of transcription factors like ELK1 and ATF2.

Accordingly, p38 MAPKs phosphorylate a broad assortment of proteins and it's been estimated that they might have approximately 200 to 300 substrates each. Some of the targets are downstream kinases like MAPKAPK2, which can be activated through phosphorylation and further phosphorylate additional targets.

Gene Information

Human
Gene Name:	MAPK12
Uniprot:	P53778
Entrez:	6300

Family

Belongs to:
protein kinase superfamily

Alternative Names

EC 2.7.11; ERK3; ERK-6; ERK6MAPK 12; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK12; mitogen-activated protein kinase 12; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; p38 gamma; p38gamma; PRKM12; SAPK-3; SAPK3EC 2.7.11.24; Stress-activated protein kinase 3

Molecular Weight

Mass (kDA):
41.94 kDA

Genomic Context

Human
Location:	22q13.33
Sequence:	22; NC_000022.11 (50252901..50261810, complement)

Tissue Specificity

Highly expressed in skeletal muscle and heart.

Subcellular Localizations

Cytoplasm. Nucleus. Mitochondrion. Mitochondrial when associated with SH3BP5. In skeletal muscle colocalizes with SNTA1 at the neuromuscular junction and throughout the sarcolemma (By similarity).

Diseases

• Malignant Neoplasms
• Neoplasms
• Inflammation
• Colorectal Cancer
• Cholangiocarcinoma
• Tongue Neoplasms
• Mood Disorders
• Melanoma
• Inflammatory Bowel Diseases
• Malignant Tumor Of Colon
• Myeloid Leukemia, Chronic
• Intestinal Diseases
• Carcinoma

• Carcinogenesis
• Malignant Neoplasm Of Mouth
• Myeloid Leukemia
• Uterine Fibroids
• Stress, Psychological
• Benign Neoplasm
• Rectal Carcinoma

Interacting Proteins

• DLG1
• PKN1
• PTPN4
• SCRIB
• SIAH1

Pathways

• Cell Proliferation
• Response To Stress
• Dna Hypermethylation
• Dna Methylation
• Dna Repair
• Neurogenesis
• Synaptic Transmission
• Protein Metabolic Process
• Methylation
• Regeneration
• Mismatch Repair

• Tissue Regeneration
• Metabolic Process
• Cell Growth
• Pathogenesis
• Secretion

PTMs

• Phosphorylation

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MAPK12

Boster Bio - Best Uses Of The MAPK12 Marker

Boster Bio's primary antibody research is highly cited. Moreover, Boster's antibodies have been validated on Western Blotting, Immunohistochemistry, and ELISA. The primary antibodies can be purchased in a convenient package and are suitable for many applications. These are the best uses of the MAPK12 indicator. This article explains the process of performing this test.

Research community is highly impressed by Boster's prim antibodies

Boster’s primary antibodies have been developed over 25+ years. They are highly valued by the research world. These antibodies are proven to have excellent affinity and are highly cited in peer-reviewed publications. They have been validated on ELISA, Immunohistochemistry, and Western Blotting, and are therefore highly cited in research. These antibodies can be used in a variety of research areas and are highly recommended for scientists.

Boster has several primary antibodies, including the ANTI MOUSE/HUMAN MAC-2(GALECTIN-3) antibody. This antibody is highly regarded in the scientific community, as it targets the p62 protein in human cancers. IgG, Cytosine, and Collagen are also primary antibodies. They also include HTATIP, PARP10, and IDh2.

How to record the test result with autoradiography film

Autoradiography films are used in medical and environmental sample analysis. This type of imaging technique records a radioactive structure on the film by interacting with silver bromide crystals. The emulsion develops into a photographic image, and the sample is viewed microscopically. This test allows doctors to pinpoint the exact location of affected organs, tissues, and cells.

How to get rid of primary and secondary antibodies from the membrane

There are many ways to perform a dilution blot. You can either use the PVDF or nitrocellulose membranes. You can cut strips of 1cm in the case of the PVDF membrane and label each one with a pencil. In the first method, you should run your protein sample through gel electrophoresis before transferring the membrane to the PVDF membrane. You should then gently rock the membrane to get rid of any remaining protein.

First, determine the concentration of your primary antibodies before you can do a chemotaxis test. If you use lower concentrations of your primary antibody, the exposure will take longer. You will also notice a shorter exposure time if your membrane is more mature. You will still get a blot that is optimized with a specific band of the protein of your interest and no background or non-specific bands. After performing a "chemiluminescent" blot, it is possible to either remove the membrane from the blot or to re-create the blot using adjusted dilutions.

If you are accustomed to performing western blots, you can reduce the time by varying the blocking and incubation times. If you do multiple blots of the same gel, you may reduce the primary antibody concentration to decrease multiple bands. Affinity-purified antibodies are a way to remove primary and secondary antibodies from the membrane. Additionally, the SDS boiling water buffer must contain a fresh reducing agent.

If you are doing a multi-antigen test, you should strip the membrane after each incubation. You can do a more thorough analysis by removing primary and secondary antibodies. It will also allow you remove unneeded antibodies. The type of membrane you use will determine the method used for membrane stripping. If the primary antibody cannot be removed, the secondary antibody can detect signal bands and perform a chemically luminescent analysis of the signal.

Depending on the sample, you may be able to use the same labeling method for both proteins. You can use the same primary antibody for both blots but use different amounts. This allows you check for any differences in the target protein. You can use the same technique even if you are using a different primary antibody. You can then use the secondary antibody on that secondary antibody to see if it matches the target protein.

There are several reasons why a certain primary antibody does not react with the second one. One reason incompatibility is most often due to the fact that the primary antibodies doesn't have enough affinity for the protein of concern. You can use a higher concentration of the secondary antibody, or incubate for longer periods at 4oo. You can use a switch-blocking reagent such gelatin or milk if your primary antibodies and secondary antibodies cross-react.