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1 Citations 3 Q&As
Facts about DNA/RNA-binding protein KIN17.
May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing.
Mouse | |
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Gene Name: | Kin |
Uniprot: | Q8K339 |
Entrez: | 16588 |
Belongs to: |
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KIN17 family |
antigenic determinant of recA protein (mouse) homolog; Binding to curved DNA; BTCDDNA/RNA-binding protein KIN17; KIN, antigenic determinant of recA protein homolog (mouse); KIN, antigenic determinant of recA protein homolog; KIN17antigenic determinant of recA protein homolog
Mass (kDA):
44.722 kDA
Mouse | |
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Location: | 2|2 A1 |
Sequence: | 2; |
Highly expressed in transformed mouse AtT20 neuroendocrine cells. Expressed at a lower level in testis, kidney, skeletal muscle, liver, lung, spleen, brain and heart and kidney. In testis, expressed at much higher levels in proliferating cells than in differentiating cells. Not detected in embryo.
Boster Bio offers many products that can be used to determine the concentration of the KIN marker within biological samples. Pipettes can be used for preparing samples ranging in size from 0.5 milliliters to 1 milliliter. Pipettes with multiple channels should always be used for the preparation of large amounts of samples. Boster scientists are able to communicate their findings even for products that are not validated with other scientists and receive product credits.
Boster Bio's PICOKINE(tM), ELISA kit allows you to quickly measure the levels of soluble protein in biological samples. Boster Bio is an expert in coating ELISA plates with high-affinity antibodies. The ELISA kit includes a step-by-step guide to prepare the sample for the ELISA.
These Boster Picokine(tm) ELISA kits are solid phase immunoassays using micro-titration wells that are removable to deliver amazing results in only 90 minutes. They offer a fast reliable, efficient, and convenient way to analyze biological samples. The PicoKine(tm) Quick ELISA has been tested for a range of target analytes and has a fast turnaround time.
Primary antibodies are made from the immune system of a host, and they contain complementarity-determining regions (CDRs). For more information, go to Wikipedia's page on CDRs. Scientists make polyclonal antibodies by immunizing host animal with a particular antigen, then obtaining the antibody from the host's blood or egg. The antibody is utilized to recognize the antigen's presence on the target cell's surface.
Research studies employ monoclonal antibodies quite extensively. They have several advantages, including high specificity, very low cross-reactivity, little lot-to-lot variation, and a small number of non-specific side effects. Monoclonal antibodies were originally developed in mice. However, technological advancements have allowed them to be developed in other species. Boster Bio's KIN marker for primary antibodies can aid researchers to better determine the specificity of their monoclonal antibodies.
Boster's anti-beta actin rabbit monoclonal antibody can be a valuable internal control for western blotting. It helps to identify the primary antibody within tissue. It is used to determine the presence or absence of retinol-binding proteins 4 in porcine cells. And with Boster's antibody-purity control kits, a reliable detection system is at your fingertips.
The primary antibodies must match the species of the sample being examined. The F(ab) is a common component in tissues, binds to the endogenous antibody present in the sample and blocks the binding of secondary antibodies. However this method does not provide a complete block, and leaves some background. Below are a few of its shortcomings. It is important to note however that the KIN marker can provide reliable results if used properly.
To increase the number of anti-SARS-CoV-2 memory cells in the serum of patients with SARS virus This marker is especially important in vaccine studies. It aids researchers in predicting anti-SARS-CoV-2 antibodies which are specific to the virus. The KIN marker can also be used for research purposes particularly in clinical trials. These findings are a significant contribution to the field of vaccine research.
This method is versatile and can be utilized in a variety of scenarios. It is cheaper than other methods, doesn't require the process of purification or the use of huge amounts of organic solvents and is also more affordable. Despite its widespread use and low cost however, the KIN marker isn't completely reliable. Many factors can hinder the enzyme's activity , and the results can vary depending on the stability of the antigen.
A series of graduation lines on a pipette permit you to measure more volume in one measurement. The tip of a pipette can be used to measure the volume of the sample. If you draw a sample from the zero mark the marker will indicate that 3.19 milliliters was delivered. You can utilize this information for a variety of pipetting operations. Unlike drinking straws, pipettes are not designed to be used in the mouth. To ensure precise measurements, you should always make use of a pipette.
The danger of repetitive strain injuries is reduced with the use of an electronic pipette. Pipettes are a crucial part of scientific experiments. To minimize strain injuries from pipetting, they must be designed to be ergonomically. Electronic pipettes can also offer automatic volume control. By following the guidelines they enable you to pipette more accurately and quickly. When used properly pipettes can make the entire process much easier.
It is essential to be familiar with the various kinds of pipettes available when you are looking for one. There are many kinds of tips available, each with its own function. Some of them are meant to be used in different ways. Using the right tip can guarantee more precise measurements and better results. Make sure to follow the instructions of the manufacturer when choosing a pipette tip. Refer to a guide if in doubt of the difference between tips.
Pipettes generally fall into five categories. There are five types of pipettes: repeat, disposable transfer, graduated, and multichannel. Each class has its own specifications and guidelines. The accuracy of laboratory tests is dependent on the usage of pipettes. It is important to know how to operate all equipment in the lab correctly. If you have any doubts about how to use an instrument, consult an QA manager.
A graduated tip can reduce the effects of capillary action and regulates the temperature of the tip and sample. It also helps prevent repetitive joint injuries and stress injuries. The KIN marker is particularly efficient for pipetting in hot and humid conditions, as it blocks liquid from sticking to the orifice. Be sure to re-wet your tips after using the KIN marker.
Precision and accuracy are crucial in biomedical research. To ensure that your results are accurate you must accurately measure the amount of liquids and substances. Pipettes allow you to precisely measure the exact amount of liquids and chemicals. This will result in more reproducible and accurate results. Therefore, you must learn how to use pipette tips in your experiments! You'll be successful when you practice the correct pipette technique!
A KIN marker is an excellent tool for many medical research applications. It permits researchers and doctors to keep sensitive areas free of contamination. Dental procedures, for instance are often involving sensitive tissue that needs to be exposed to a sterile environment. Pipettes help you accurately apply dental products to these areas and stop the growth of bacteria and other contaminants. In certain instances it is required for research or patient care.
PMID: 1923796 by Angulo J.F., et al. Identification and expression of the cDNA of KIN17, a zinc-finger gene located on mouse chromosome 2, encoding a new DNA-binding protein.
PMID: 1715759 by Angulo J.F., et al. Identification of a mouse cDNA fragment whose expressed polypeptide reacts with anti-recA antibodies.
*More publications can be found for each product on its corresponding product page