Importin subunit beta-1 (KPNB1)

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in freight substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor.

Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus into the cytoplasm where GTP hydrolysis releases Ran from importin.