This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
1 Citations
Facts about Importin subunit alpha-5.
Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus into the cytoplasm where GTP hydrolysis releases Ran from importin.
Human | |
---|---|
Gene Name: | KPNA1 |
Uniprot: | P52294 |
Entrez: | 3836 |
Belongs to: |
---|
importin alpha family |
Importin alpha 5; importin subunit alpha-1; importin-alpha-S1; IPOA5; karyopherin alpha 1 (importin alpha 5); Karyopherin subunit alpha-1; KPNA1; NPI-1; NPI-1SRP1importin alpha 5; Nucleoprotein interactor 1; RCH2; RCH2RAG cohort protein 2; recombination activating gene cohort 2; SRP1; SRP1-beta
Mass (kDA):
60.222 kDA
Human | |
---|---|
Location: | 3q21.1 |
Sequence: | 3; NC_000003.12 (122421902..122514939, complement) |
Expressed ubiquitously.
Cytoplasm. Nucleus.
You have found the best anti-KPNA1 antibodies. This article will talk about the Boster bio KPNA1 marker as well as its uses. This article will also discuss the PCR primers used to clone specific mutations. This article is for both new and experienced PCR users. We hope that you will find this article useful.
The KHDRBS2 gene is involved in alternative splicing, which regulates mRNA levels. Its phosphorylation by the protein PTK6 inhibits its ability to bind to RNA and influences the splicing of mRNA. Scientists can monitor these genes' changes by using antibodies. Boster Bio offers monoclonal and multiclonal antibodies for KHDRBS2. Boster Bio's Anti-KHDRBS2 antibody reacts both with Human and Mouse samples.
Boster Bio Anti-Bax S4 Antibody reacts with human, mouse and rat samples. This antibody comes in 100-ul Liquid. The anti-Bax antibody was validated using positive and negative samples. It can be ordered by calling 1-855 BOSTER For more information, please visit the manufacturer's web site.
PCR primers are widely used for cloning and chimeric gene expression. They are also useful for diagnostic testing. The PCR primers are designed for an anneal to the reverse complement sequence. In the reverse complementary scheme, A's pair together with T's while C's pairs with G's. To generate the correct PCR primers, it is necessary to compile a list of complementary nucleotides.
Three steps are common for PCR reactions. Initial denaturation involves temporarily increasing the reaction temperature up to 95 degrees Celsius for a short amount of time. This step is necessary to separate the complex double-stranded DNA molecules and prevent damage to the template. After the initial denature, the DNA polymerase expands the reaction at 72° Celsius (the optimal temperature for amplifying the DNA molecules), followed by extension.
LM-PCR was used to clone a region upstream of the rpsL gene. The LM-PCR primers PV (5'-TCTCCGTTGACCAGTTTTG-3'), PVI (5'-GTTTTTTTTCGGCGTCATG-3'), and a Southern hybridization reaction using the rpsL gene as a probe. The 5’ end of the rpsL genes was digested in BclI. It was then ligated into a chromosomal DNA containing BclI.
After the DNA has been successfully cloned and ligated, it should be purified by a restriction protein and checked for amplification by running a gel. If a PCR reaction produces a strong band, the DNA has successfully cloned. Next, DNA Ligation is used to fuse DNA to the recipient DNA. After the ligation has been completed, DNA Ligation is used to fuse the insert and the recipient plasmid.
A PCR is used to detect mutations in a gene sequence or gene. These mutations can be caused by many factors, including inherited diseases, environmental toxins, or environmental contamination. PCR primers are designed by a high-throughput pipeline based on sequence alignments and hairpin structures. PCR primers can also serve as a tool for protein interaction experiments.
A reference sequence must be known in order to amplify the cDNA in the case of KPNA1. Primers should be designed to target the gene to be studied in order to achieve this. The primers should be short and contain a high GC content (40-60%). They should also contain no more than three C or G residues in the 3' end of the primer, and should avoid any intermolecular complementary sequences. Additionally, primers shouldn't contain intramolecular areas of secondary structure because they can interfere with primer Annealing.
Primers for amplification of the KPNA1 gene are highly flexible and can be designed to target a specific mutation. The primers should be optimized to each template and temperature. A primer pair that is longer than another may result in a larger band. After 20-40 cycles, the product can be analyzed. Then, further experiments can be performed.
The PCR primers for the KPNA1 marker must be labeled. They should be able to detect specific mutations. For this, a housekeeping gene is used to provide an internal standard. Its role it to ensure the quality and safety of the target. Because primers are not labeled, the housekeeping gene approach is more complicated. The target gene must be accurately matched before the PCR is performed.
M-MLV RNase H minus enzymes can be purchased in various forms. There are two types, one is a deletion mutant, and one is a point. Native M-MLVRT is recommended for 37degC. Point mutation primers are more stable at higher temperatures and can be used for tests involving specific KPNA1 mutations.
PMID: 7831767 by O'Neill R.E., et al. NPI-1, the human homolog of SRP-1, interacts with influenza virus nucleoprotein.
PMID: 8052633 by Cortes P., et al. RAG-1 interacts with the repeated amino acid motif of the human homologue of the yeast protein SRP1.
*More publications can be found for each product on its corresponding product page