Lysine--tRNA ligase Antibodies

AND KARS1 ELISA kits, Lysine--tRNA ligase Proteins

Many biological assays use antibodies to detect Lysine--tRNA ligase, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Lysine--tRNA ligase in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Lysine--tRNA ligase antibodies...

We validate the specificity of these antibodies to Lysine--tRNA ligase by testing them on tissues known to express KARS1 positively and negatively. Browse below to find the KARS1 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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KARS1 Infographic by Boster Bio

All KARS1 Products

Product

Figures

Specificity

Applications

Price

Human KARS Recombinant Protein

SDS-PAGE

$250
Cat # PROTQ15046
Starting from 20ug

Anti-LysRS Rabbit Monoclonal Antibody

Human, Mouse, Rat

IP, IF, IHC, ICC, WB

$370
Cat # M02479-1
100 μl/vial

Lysyl tRNA synthetase (KARS) (NM_005548) Human Over-expression Lysate

Human

$628
Cat # LS003735
100 µg

KARS1 Overview

Facts about Lysine--tRNA ligase.

KARS1 3D structure. Source: alphafold

Lysine--tRNA ligase (KARS1)

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step response: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690).

Catalyzes the synthesis of this signaling molecule diadenosine tetraphosphate (Ap4A), and thus mediates disturbance of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739). .

Gene Information

Human
Gene Name:	KARS1
Uniprot:	Q15046
Entrez:	3735

Family

Belongs to:

class-II aminoacyl-tRNA synthetase family

Alternative Names

Lysine--tRNA ligase

Molecular Weight

Mass (kDA):
68.048 kDA

Genomic Context


Human

Location:	16q23.1
Sequence:	16; NC_000016.10 (75627724..75647687, complement)

Subcellular Localizations

[Isoform Cytoplasmic]: Cytoplasm, cytosol. Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted. Secretion is induced by TNF-alpha (PubMed:15851690). Cytosolic in quiescent mast cells. Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739).; [Isoform Mitochondrial]: Mitochondrion.

Diseases

Charcot-Marie-Tooth disease, recessive, intermediate type, B (CMTRIB)

Interacting Proteins

AIMP2
EPRS
RPSA

PTMs

Acetylation

Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/KARS1

References

PMID: 9278442 by Shiba K., et al. Human lysyl-tRNA synthetase accepts nucleotide 73 variants and rescues Escherichia coli double-defective mutant.

PMID: 10952987 by Tolkunova E., et al. The human lysyl-tRNA synthetase gene encodes both the cytoplasmic and mitochondrial enzymes by means of an unusual alternative splicing of the primary transcript.