IRS2 Antibodies

Insulin receptor substrate 2 (IRS2)

May mediate the control of various cellular processes by insulin. .

Gene Information

Human
Gene Name:	IRS2
Uniprot:	Q9Y4H2
Entrez:	8660

Family

Belongs to:
No superfamily

Alternative Names

insulin receptor substrate 2; IRS2; IRS-2

Molecular Weight

Mass (kDA):
137.334 kDA

Genomic Context

Human
Location:	13q34
Sequence:	13; NC_000013.11 (109752695..109786583, complement)

Subcellular Localizations

Cytoplasm, cytosol.

Diseases

• Insulin Resistance
• Diabetes Mellitus
• Diabetes Mellitus, Non-insulin-dependent
• Obesity
• Insulin Sensitivity
• Malignant Neoplasms
• Hyperinsulinism
• Neoplasms
• Hyperglycemia
• Impaired Glucose Tolerance
• Diabetes Mellitus, Experimental
• Liver Carcinoma

• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Inflammation
• Janiceps
• Carcinoma
• Hyperplasia
• Polycystic Ovary Syndrome

Interacting Proteins

• PIK3R1
• SIRT1
• YWHAZ

Pathways

• Secretion
• Insulin Secretion
• Glucose Homeostasis
• Transport
• Glucose Transport
• Cell Proliferation
• Pathogenesis
• Cell Growth
• Aging
• Cell Cycle
• Localization
• Gluconeogenesis
• Translation

• Cell Migration
• Hypersensitivity
• Cell Death
• Lactation
• Energy Homeostasis
• Rna Interference
• Cell Motility

PTMs

• Phosphorylation
• Cleavage
• Dephosphorylation
• Ubiquitination
• Oxidation
• Reduction
• Deacetylation

• Acetylation
• Methylation
• Myristoylation
• Amidation
• Acylation
• Nitration
• Glycosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/IRS2

Best Uses Of The IRS2 Marker In Boster Bio ELISA Kits

This article will discuss the various types of antibodies employed by Boster Bio. This article will also offer tips for optimizing your ELISA experiments. Detecting proteins using primary antibodies is a great method to determine which antibodies are the most effective for your experiments. In this article, we'll talk about the basics of the IRS2 marker and how you can enhance your Boster Bio experiments to detect proteins.

Boster Bio is a biotech company that makes primary antibodies.

If you're in search of primary antibodies to use in a specific study, you've come to the right place. Boster Bio's inventory of antibodies contains hundreds of primary antibodies. The company's proprietary ELISA platform, PicoKine(tm), delivers high-sensitivity ELISA kits. Since 1993, the company has developed antibodies for a variety of applications. It's also one of China's biggest catalog antibody companies.

Boster's primary antibody was designed with scientists in mind. They have high specificity and affinity. With a 25-year track record they are a trusted brand by researchers. In addition to being highly specific and high affinity, Boster antibodies have undergone testing in immunohistochemistry as well as Western Blotting. The company also rewards scientists who have reviewed its products first time. Scientists around the world can get product credits for their first review and to confirm the quality of its products.

Because they are designed to identify the same antigens that are found in diverse species, the primary secondary antibody system permits dual-labeling. This allows researchers to inquire more about the specimen using a single antibody, while getting context-specific information and accurate answers. So primary antibodies are an integral part of any research program. This kind of antibody is used for molecular-level diagnosis of many illnesses.

Primary antibodies are immunoglobulins that originate from the immune system of their host. Their quality is determined by their affinity and specificity. Both are defined by the quality of their non-covalent bond with the antigens they target. A good primary antibody can detect, purify, and quantify a specific antigen in a proper manner. The question is whether primary antibodies are the best? The answer is yes and here are three main reasons why.

Tips for optimizing your Boster Bio

If you are using antibodies against IRS2 marker, you may be wondering what the optimal flow protocols are. There are many optionsavailable, and these suggestions will aid you in choosing the best protocols for your experiment. Boster Bio has troubleshooting guides for those who aren't sure what to do with an experiment. These guides will help you get the results you want.

Antibodies that detect proteins using primary antibodies

Detecting proteins using primary antibodies with the taxonomy IRS2 is the most well-known method to determine the expression of proteins in cell culture. It lets you stain multiple targets in one step. It can be used to study protein expression in multiplex IF/ICC experiments. It functions by affixing primary antibody to an fluorescent dye, which allows multiple targets to simultaneously be identified within the same sample.

We used the anti-IRS2 monoclonal antibodies in sodium HEPES 10 mM (pH 7.5) 150 mM NaCl, and 100 mg/ml BSA. This antibody detects the endogenous levels of IRS2 however, it is not able to cross-react to the IRS-1 protein. The primary antibody was prepared by immunizing animals using the sequence of peptides. The antibody was purified using peptide affinity chromatography and protein A affinity chromatography.

The detection of proteins with primary antibodies that are bound to the DNA domain of IRS2 requires an additional label for the detection of the testicular and ovarian cells. However, in both cases IRS2 is essential in the development of the testicle. While other IRS proteins aid in the growth of testicular tissue The current research supports the importance of IRS2 within the testis. It has been proven that Type 2 diabetics have lower levels of IRS2 expression. This is a clue to studies that study the effects of diabetes on male reproductive function.

Primary antibodies were used to probe membranes overnight, at temperatures of four degrees Celsius. Secondary antibodies were added to diluted Tris-buffered Sline (TBS), or 5 percent dry milk that is nonfat. Once the primary antibodies had been bound to the target protein, they were then incubated with DAPI. This staining process is repeated three or two times for each sample. Then, they were rinsed with PBS 4 times before being analyzed for signal/background ratio.

The most effective method of detecting IRS2 protein expression is to use primary antibodies and taxonomy. The normal function of the inner ear and hearing is dependent on IRS proteins. Deaf mice are affected by MAPK phosphorylation. A study on mice that lack IRS2 also revealed that the IRS2 gene regulates PTP1B activity which could cause the development of diabetes-related sensory syndromes.

WT and Irs2-/D mice share the same expression of the IRS2 gene However, their relative levels aren't the same. The RT-PCR method was also used with TaqMan probes, and the results were normalized to Gapdh. To determine the expression of IRS2 protein tests were homogenized using lysis buffer. Each lane was loaded with 20 grams of total protein. Each blot was probed using the antibodies that were used.