HPD Antibodies

4-hydroxyphenylpyruvate dioxygenase (HPD)

Key enzyme in the degradation of tyrosine. .

Gene Information

Human
Gene Name:	HPD
Uniprot:	P32754
Entrez:	3242

Family

Belongs to:
4HPPD family

Alternative Names

4HPPDEC 1.13.11.27,4-HPPD; 4-hydroxyphenylpyruvate dioxygenase; GLOD3; glyoxalase domain containing 3,4-hydroxyphenylpyruvic acid oxidase; HPPDASE; PPD

Molecular Weight

Mass (kDA):
44.934 kDA

Genomic Context

Human
Location:	12q24.31
Sequence:	12; NC_000012.12 (121839527..121888611, complement)

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Pituitary Diseases
• Malignant Paraganglionic Neoplasm
• Dystonia Disorders
• Noise-induced Hearing Loss
• Malignant Squamous Cell Neoplasm
• Adenocarcinoma
• Mammary Neoplasms, Experimental
• Neoplasm Metastasis
• Glioma

• Brain Neoplasms
• Lung Neoplasms
• Malignant Neoplasm Of Gallbladder
• Parkinson Disease
• Bladder Neoplasm
• Tyrosinemias

Pathways

• Secretion
• Localization
• Excretion
• Transport
• Cell Death
• Prolactin Secretion
• Pathogenesis
• Sensitization
• Cell Killing
• Regeneration
• Parturition
• Cell Growth
• Cell Proliferation

• Ovulation
• Immune Response
• Aging
• Cell Cycle
• Coagulation
• Glomerular Filtration
• Proteolysis

PTMs

• Oxidation
• Phosphorylation
• Cleavage

• Hydroxylation
• Acylation
• Nitration

• Reduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HPD

Boster Bio - Best Uses Of The HPD Marker

Boster Bio offers a wide range of high-quality primary antibody products. Boster's antibody are tested for quality. They come in many sizes. This tool can be used to optimize your experiments. These are some tips and tricks that will help you get the most out of your HPD marker.

Boster's primary antibodies come in a wide variety of sizes

Boster Bio specializes on the production of polyclonal, high-quality antibodies. Boster Bio stocks antibodies for IHC (WB), ELISA, FC, FC, and Flow. The company offers a wide range of products, including primary and rabbit polyclonal antibodies. Boster Bio also sells kits and reagents that are suitable for many applications and assays. Boster Bio offers a Boster quality guarantee for all its products.

The concentration of a primary antigen is an important aspect to consider when choosing one. In an ELISA, a minimum amount can be used as it will reduce background and nontarget binding. The signal intensity can also be affected by positive and negative controls as well normalization reference. You can also reduce background by increasing buffer volume, wash duration, as well as frequency.

You can purchase secondary antibodies in liquid or powder form. Liquid antibodies are shipped as concentrates and may need optimizing before use. Typically, these antibodies need to be stored at a temperature between four and eight degrees Celsius. Liquid antibodies can be kept in the freezer for up one year, then thawed before use.

Positive and negative controls are also essential to testing antibodies for specificity. Positive controls include purified proteins and negative controls are tissues from knockout animals. Some companies even sell peptides which bind to the epitopes identified by the antibody. This way, researchers can ensure that their antibodies truly are specific. When searching for an effective and efficient antibody make sure you verify its quality.

Secondary antibodies are an important component of the process. There are many ways secondary antibodies can be combined with labels, depending on their downstream applications. There are two main types of secondary antibodies: unconjugated and conjugated. Depending on the purpose of the secondary antibody, and the signal desired, you can choose which one to use. While HRP and Phosphase are both sensitive, aqueous lysis buffer works better.

Boster primary antibodies are tested to ensure quality

In addition to producing quality antibodies, Boster Bio also manufactures ELISA kits and reagents. Boster Bio's proprietary process ensures that antibodies, reagents, and primary antibodies work as expected. Boster antibodies have been optimized for multiplex, WB, ELISA and multiplex assays. The company also offers multiplex immunohistochemistry and archives their data at a very low cost.

Boster uses a BCA assay to test each primary antibody for purity and consistency. To test the concentration of each antibody, Boster uses a whole cell lysate from skeletal muscle. The sample is then diluted with Dithiothreitol, and heated for five min at 100EsC. The GAPDH (and beta-actin) antibodies are then used in Western blot analysis. In every lot, the levels of antibody expression for each protein are constant.

Check for details about the validation process when evaluating the quality Boster's prim antibodies. Quality is extremely important. For example, antibodies should be tested for cross-reactivity with closely related molecules and for specificity against a library of in-house developed antigens. For maximum stability storage and shipping, it is important to lyophilize antibodies. They must also be subject to strict quality control.

Boster's primary antibody are subject to rigorous testing, in addition to quality control and validation. MIAPAR-compliant antibodies have been validated using the same assays used by the users. Users must design experiments using their primary antibodies. After analyzing the results, they should verify that they are reproducible. To improve antibody quality and reproducibility, all three parties must work together. For this, scientists should work together with the antibody vendors and developers to standardize the entire process.

Boster Biological Technology Co., Ltd. utilizes a BCA-protein assay kit for validation of primary antibodies. To detect primary antibodies, it uses spectrally distinct fluorophores. Enzymatic detection is added to the method. This can increase the multiplex capability of a Chemiluminescent Imaging System. These methods can also be used to test for other antibodies.