HNRNPL Antibodies

Heterogeneous nuclear ribonucleoprotein L (HNRNPL)

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements (PubMed:11809897, PubMed:22570490, PubMed:24164894, PubMed:25623890, PubMed:26051023).

Part of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and correlated with the majority of nascent transcripts (PubMed:2687284). Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter (PubMed:11809897).

Gene Information

Human
Gene Name:	HNRNPL
Uniprot:	P14866
Entrez:	3191

Family

Belongs to:
No superfamily

Alternative Names

FLJ35509; heterogeneous nuclear ribonucleoprotein L; hnRNP L; hnRNP-L; HNRPL

Molecular Weight

Mass (kDA):
64.133 kDA

Genomic Context

Human
Location:	19q13.2
Sequence:	19; NC_000019.10 (38836370..38852347, complement)

Subcellular Localizations

Nucleus, nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. These granules are not identical with P bodies or stress granules.

Diseases

• Glioblastoma Multiforme
• Ataxia
• Glioblastoma
• Hypoxia
• Tissue Adhesions
• Spermatogenesis Abnormal
• Physiological Stress
• Malignant Neoplasms
• Nervousness
• Azoospermia

• Inflammation
• Ataxia, Spinocerebellar
• Anoxia
• Congenital Absence Of Germinal Epithelium Of Testes
• Neoplasms, Nerve Tissue

Interacting Proteins

• HNRNPA2B1
• ILF3
• VHL

Pathways

• Translation
• Cell Adhesion
• Dna Repair
• Pathogenesis
• Spermatogenesis

PTMs

• Cleavage

Research Areas

• Core ESC-Like Genes
• DNA Repair
• DNA replication, Transcription, Translation and Splicing
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HNRNPL

Boster Bio - Best Uses Of The HNRNPL Marker

A simple reagent like HNRNPL markers could prove to be an effective tool when working with cell culture media. Boster pipettes are able disperse aqueous solutions from 0.5 to 1 ml. A multichannel pipette is recommended for large samples. This marker is not approved for use by humans, however, it is widely utilized in the research and development of cell-culture media.

Primary antibodies with high affinity

Flow Cytometry uses particles or cells as a sample. Boster's high-affinity antibodies are useful in a variety research applications. Monoclonal and polyclonal antibodies are available. Boster antibodies have been well reviewed and have been in stock for more than 25 years. Learn more about the numerous benefits of high-affinity prima antibodies from Boster Bio.

The quality of primary antibodies is based on two main features: specificity and affinity. Specificity refers the strength of the noncovalent bond formed between primary antibody and antigen. A high-quality antibody will have minimal or no binding to antigens that are not intended. It will be useful for the detection, purification, and measurement applications if it has the highest degree of specificity.

In the case of PRRSV the high-affinity primary antibody binds to GRS and reduces the cDNA band that was expected to be 200 bp in size. The band was not diminished by a normal IgG. A HnRNP F antibody exhibits a strong interaction with GRS in the antigenomic RNA of PRRSV virus.

Secondary antibodies

To identify HNRNPL in leukemia cells we performed secondary antibody tests. This antibody recognizes the CA repeat (CA) on the DNA molecule that is single-stranded. The affinity of HNRNPL is directly proportional to the CA repeats. The HNRNPL marker was evaluated by comparing the expression of proteins of BUB1 and HNRNPL in longitudinal samples of PBMCs and B-CLL cells in pure form. The study was conducted in nine patients. We found that BUB1 proteins increased with time.

This particular antigen is made by several manufacturers of antibodies. In this study, we used the anti-mouse pSC35 antibody produced by Santa Cruz Biotechnology. LifeSpan Biosciences produced another antibody. The antibodies were reverse transcribed using an Magna RIP Kit. To determine the activity of firefly's luciferase it was tested using RNA. To avoid plasmid contamination, we normalized Renilla Luciferase activities to Luciferase.

A secondary antibody that employs the HNRNPL marker is effective in finding BUB1 mRNA in the RNA immunoprecipitated in HG-3 cells. Secondary antibodies utilizing the HNRNPL marker detect BUB1 mRNA in the 3'UTR. These antibodies were used to identify the immunoprecipitated the RNA using a Student t-test. Alternately, BUB1 protein expression levels could be normalized with respect to b–actin.

ELISA kits

Boster Bio is a specialist manufacturer of antibodies, focusing in high-sensitivity and high-specificity antibodies. The company has been perfecting their methods and technologies for two decades and has over 29,000 publications to prove it. To ensure that they're suitable for ELISA or WB applications Their antibodies have been tested quantitatively against recombinant protein as well as cell lines. Here are some characteristics of Boster Bio HNRNPL ELISA kits:

This kit includes a monoclonal anti-VHL von Hippel-Lindau tumour suppressor. It reacts with VHL in a number of animal models and connects to the hydroxylated hypoxia-inducible factor. It also interacts with HIF1A and HIF1AN and it ubiquitinates ADRB2.

Histological background

Histology is a major branch in biology that studies cells and tissues. This branch of biology uses various methods for microscopic analysis, including staining and sectioning. The field has many applications that include autopsy, forensic investigations, and diagnosing diseases. In addition to these functions, histology is widely used in medical research, where scientists can utilize it to discover the cause of disease.

Applications of the HNRNPL marker

The HNRNPL marker is a crucial marker for research into cancer. Its sequence is in alignment with the E2F3 promoter, SLFN12L TSS, and the intronic region of DNAH7. This unique sequence permits accurate diagnosis of cancers using their expression levels. Applications of the HNRNPL marker include cancer diagnosis, prognostication, and personalized treatment.

This study was carried out using the EZMagna CHIP A/G kit by Millipore. Then 5 7 x107 cells were isolated and washed in RIP the lysis buffer. The RNA was taken from the cells, and then tested to qPCR in real-time. The primers are listed in Supplementary Table 3. The efficiency of transfection of the primers was determined by using a firefly/renilla Luciferase test.

HnRNP L is one of the members of the hnRNP family and is an essential nuclear protein that plays a role in splicing, transcription transport, and the degradation of precursor mRNAs. Although its role in Pca is not clear It has been found to be expressed differently in lung cancer. Although the exact function of HNRNPL in Pca is still unclear, it is known that it regulates lung cancer's potential to cause tumors.