HIC1 Antibodies

Hypermethylated in cancer 1 protein (HIC1)

Transcriptional repressor. May act as a tumor suppressor.

May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses.

Gene Information

Human
Gene Name:	HIC1
Uniprot:	Q14526
Entrez:	3090

Family

Belongs to:
krueppel C2H2-type zinc-finger protein family

Alternative Names

hic-1; hypermethylated in cancer 1; ZBTB29hypermethylated in cancer 1 protein; Zinc finger and BTB domain-containing protein 29; ZNF901

Molecular Weight

Mass (kDA):
76.508 kDA

Genomic Context

Human
Location:	17p13.3
Sequence:	17; NC_000017.11 (2055103..2063241)

Tissue Specificity

Ubiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.

Subcellular Localizations

Nucleus.

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Cell Transformation, Neoplastic
• Malignant Paraganglionic Neoplasm
• Colorectal Cancer
• Colorectal Neoplasms
• Mammary Neoplasms
• Malignant Neoplasm Of Breast
• Carcinogenesis
• Microsatellite Instability
• Leukemia

• Medulloblastoma
• Leukemia, Myelocytic, Acute
• Adenocarcinoma
• Malignant Tumor Of Colon
• Myeloid Leukemia
• Brain Neoplasms
• Polyps

Interacting Proteins

• ARID1A
• CTBP1
• Ctbp1
• CTBP2
• Ctbp2

• TCF7L2

Pathways

• Methylation
• Dna Methylation
• Pathogenesis
• Dna Hypermethylation
• Gene Silencing
• Cell Cycle
• Demethylation
• Cell Proliferation
• Localization
• Rna Interference
• Dna Hypomethylation
• Aging
• Dna Demethylation

• Regulation Of Gene Expression
• Oncogenesis
• Dna Repair
• Senescence
• Drug Resistance
• Mismatch Repair
• Angiogenesis

PTMs

• Methylation
• Demethylation
• Deacetylation
• Acetylation

• Sumoylation
• Phosphorylation
• Reduction
• Glycosylation

Research Areas

• Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HIC1

Best Uses Of The HIC1 Marker

Steven Boster, a biotechnology entrepreneur, launched his first product in 1993. He earned the nickname "the man who converts science into the lavatory". Since then, Boster has developed hundreds of primary antibodies and is now the largest antibody catalog company in China. He also developed a proprietary ELISA platform called PicoKine(tm). This technology enables delivery of high-sensitivity ELISA tests, which are based in part on his trade secrets.

ABAQUS code used to design a cylindrical HIC transportation package

To ensure the integrity of a cylindrical HIC transportation package, three-dimensional dynamic structure analysis must be used. The package must be structurally sound under a free fall of 9 meters and a puncture of 1 m. To evaluate the package's integrity under these conditions, the authors performed a detailed analysis using the ABAQUS code.

The ABAQUS code is used in Finite Element Analysis (FEA). First, the user will need to input data to the finite element analyzer, also known as the "solver". Second, processing is required to generate the output visual file. Third, post-processing is required to generate the final output file, which may be a report, animation, or image.

ISH signal intensity

The HIC1 gene encodes the protein that is expressed in many types of cancer cells. It is known to be highly specific for cancer cells. Boster Bio has created ELISA kits to measure this protein in a variety tissue types. PicoKine(tm), a proprietary platform, delivers high-sensitivity ELISA kit kits. Here's a description of how to use HIC1 markers.

lncRNA-based probes

Two lncRNA marks were selected in order to validate the lncRNA probes using formalin-fixed paraaffin-embedded tissue. These were GLIS2-AS1 or PVT1. TSINGKE Biological Technology manufactured digoxigenin-labelled primers. Incubation was carried out in a 37deg incubator using lncRNA probes. BCIP/NBT was used to visualize.

The RNA-based LNCRNAs have different cellular localization patterns. The validated probe set contained 34 lncRNAs. The lncRNA cell pairs were further divided into I-V. Each pair was presented with a different type of cell or class. Five lncRNAs may be found in different cell types. The top-left picture is the reference for the scale bar.

Among the 61 lncRNAs studied, MEG3 and XLOC-003526 colocalized in hLFs and hFFs. These two molecules share high-quality overlap in approximately 80% of cells. Similarly, MALAT1 co-localize in different cell types. These data suggest lncHOXA1 might regulate the neighboring Hoxa1 Gene in cis.

XLOC_003562 is also an lncRNA and has been shown high expression in hFFs. This lncRNA has strong correlation with nearby genes. Moreover, this lncRNA has a significant effect on the cellular volume of cells. Two daughter cells expressing high levels of NR_029435 suggest cytological abnormalities in HeLa cells.

This study suggests that lncRNAs could provide many regulatory functions and other mechanisms in different cancer types. It is important to note that many of the genes detected in the studies were previously analyzed for miRNA expression levels in PCa. However, lncRNAs could provide unique insights into the development and origin of parathyroid cancers. There is increasing evidence to suggest that lncRNAs play a role controlling the expressions of mRNAs in these tissues.

Boehringer Ingelheim Stiftung

HIC1 is an important target in cancer research. Researchers are already able identify these cells using this marker. Bio-Synthesis Inc. was responsible for genotyping DNA from human cell lines. The 293T cells were grown in DMEM with 10% FBS and 1% penicillin-streptomycin, while the DLBCL cell line was grown in Iscove's medium with 10% FBS and 10 mM HEPES.

Paul-Ehrlich-Institut

The Universitatsmedizin Mainz was restructured, and went through a phase active networking and faculty planning. This centralized decision was a success. It established a solid foundation for participation in large research centres such as the German Heart Centers. Universitat Frankfurt am Main also joined the Rhine-Main Neuroscience Network as a founding member. It is now involved in the German Heart Centers.

The institute's mission is to teach students and coordinate immunological teaching within biology, biomedical chemistry, and other areas. This research relies on the HIC1 gene. This gene is located at the surface of human cell, allowing researchers the ability to determine how many human cells are present in a given body. Molecular genetics allows researchers the ability to determine if a particular gene is associated or unrelated to a disease.

The Institut fur Histologie has excellent facilities for molecular biology and live imaging. The institute also collaborates with the Electronenmikroskopiezentrum of the University. This institute also has facilities for tier-experimental and utero electroporation. Researchers can better understand the role played by the HIC1 gene in aging by identifying the molecular and cellular mechanisms.

Neuropathology Abteilung staff includes 2 doctors. It is Rheinland’s only Neuropathology Training Facility. It employs cutting-edge methods for molecular and morphological analysis. The Abteilung's neuropathologists focus on tumors, nerves, and are trained in morphological and molecular methods to grade them.