GPI Antibodies

Glucose-6-phosphate isomerase (GPI)

Besides it's function as a glycolytic enzyme, mammalian GPI can be a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

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Gene Information

Human
Gene Name:	GPI
Uniprot:	P06744
Entrez:	2821

Family

Belongs to:
GPI family

Alternative Names

AMFGNPI; Autocrine motility factor; DKFZp686C13233; EC 5.3.1.9; glucose phosphate isomerase; glucose-6-phosphate isomerase; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; NLKSA36; oxoisomerase; PGI; PHI; Phosphoglucose isomerase; phosphohexomutase; Phosphohexose isomerase; phosphosaccharomutase; SA-36; Sperm antigen 36; sperm antigen-36

Molecular Weight

Mass (kDA):
63.147 kDA

Genomic Context

Human
Location:	19q13.11
Sequence:	19; NC_000019.10 (34353330..34402413)

Subcellular Localizations

Cytoplasm. Secreted.

Diseases

• Hemoglobinuria
• Paroxysmal Nocturnal Hemoglobinuria
• Tissue Adhesions
• Hemoglobinuria, Paroxysmal
• Neoplasms
• Thrombosis
• Anemia
• Antiphospholipid Syndrome
• Malignant Neoplasms
• Lupus Erythematosus, Systemic
• Polyendocrinopathies, Autoimmune
• Hemolysis (disorder)
• Nervousness

• Inflammation
• Autoimmune Reaction
• Malaria
• Anemia, Hemolytic
• Prion Diseases
• Hemorrhage
• Infective Disorder

Interacting Proteins

• TAMM41

Pathways

• Localization
• Transport
• Pathogenesis
• Glycosylation
• Secretion
• Cell Adhesion
• Endocytosis
• Cell Activation
• Virulence
• Immune Response
• Cell Proliferation
• Coagulation
• Secretory Pathway

• Proteolysis
• Complement Activation
• T Cell Activation
• Cell Migration
• Cell Death
• Glycolysis
• Cell Growth

PTMs

• Gpi Anchoring
• Cleavage
• Glycosylation
• Phosphorylation
• Acylation
• Biotinylation
• Methylation
• Deamination
• Myristoylation
• Deglycosylation

• Acetylation
• Oxidation
• Ribosylation
• Dephosphorylation
• Deacetylation
• Amidation
• Palmitoylation
• Decarboxylation
• Iodination
• Reduction

Research Areas

• Cytokine Research

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GPI

Boster Bio - Best Uses Of The GPI Marker

If you are seeking the best solution to your IHC workflow, check out this article! This article will discuss Boster Bio, GPI Marker and TBST and how you can optimize your IHC workflow. These are some helpful tips to improve your workflow. We hope that you find this article helpful. Enjoy learning! And don't forget to share it with your colleagues! We'd love to hear about your experiments!

Boster Bio

Boster Bio offers antibodies with high affinity, proven over the last 25 years in a variety of publications . It is also a reliable source of antibodies that can be used for immunostaining. Boster antibodies are validated in ELISA, immunohistochemistry, and Western Blotting. The Boster Bio guide contains detailed information about sample preparation, IHC and troubleshooting immunostaining. The guide also contains detailed details on sample preparation to get the best results.

GPI Marker

To analyze your samples for immunohistochemistry, you'll require the GPI marker. GPI markers are used to identify and extract DNA. There are many options to select from when designing reliable and efficient tests. Utilizing the right controls will minimize the risk of making errors. Follow these tips to ensure your experiments are successful using the Boster GPI marker.

TBST

GPI anchor protein (or GPI) has been identified as a promising antifungal molecule. It is capable of preventing the growth of many fungal species and has been demonstrated to also affect compartment-specific exonsplicing in trypanosomatids. It's still a work-in-progress however, it is becoming an increasingly popular diagnostic tool to identify TBST resistant fungi.

The GPI marker is anchored to cells' walls. It is involved in controlling the production of cell membrane proteins and cell wall proteins. The gel1 and Gel7 mutants show a leaky quality-control system. They increase the expression of GPI-anchored proteins to help compensate for this deficiency. For instance the gel1 mutant is afflicted with a defect that compromises integrity of its cell wall.

In mammalian cells and yeast, the biosynthesis of the GPI anchor is well understood. The process involves sequential modifications of phosphatidylinositol and the addition of sugars and ethanolaminephosphate to the precursor lipid in the ER. In yeast, EtN-P is linked to three mannose residues by genes PIG'N/MCD4 or PIG'O/GPI13.

Optimizing IHC workflow

If you're looking to optimize your IHC workflow using the GPI marker, it's important to understand the basics of mIF reproducibility. This includes the analysis of multiplexed staining, antibody evaluation and pre-analytical steps. You must also consider the specificity and determine if they were specific. The pre-analytical process also affects the marker's expression. All of these factors have an impact on IHC reproducibility.

Pathophysiology research requires tissue specimens. Technology advancement has also facilitated our understanding of interactions between cells. One of the newer technologies that are revolutionizing the process is mfIHC. Due to the high-quality of these images, optimizing and accuracy are key. Here are some helpful tips to improve your workflow using the GPI marker. This makes it easy to label and detection on a variety targets.

Any IHC-based assay requires the use of a binding molecules. The choice of binding molecule can make a significant difference in the final result as well as the reliability and interpretation of the test. Antibodies are the most commonly used binding molecules. They differ greatly in the specificity to their targets. The more specific the antibody, the less background or off-target binding will be. It is easier to interpret the results when the binding molecule is specific for the target.

Tissue samples can be obtained from a variety of sources. The biopsy, animal model or surgical procedure can provide you fresh tissue. However autopsy requires the animal to die for two hours and the autopsy specimen is more of a postmortem autolysis. Because antigens are denatured after 2 hours, the specimen must be fixed as soon as possible. This will stop the antigens from degrading and disappearing.